Computerized image analysis of cell-cell interactions in human renal tissue by using multi-channel immunoflourescent confocal microscopy

2012 ◽  
Author(s):  
Yahui Peng ◽  
Yulei Jiang ◽  
Vladimir M. Liarski ◽  
Natalya Kaverina ◽  
Marcus R. Clark ◽  
...  
Keyword(s):  
Image Analysis ◽  
Confocal Microscopy ◽  
Renal Tissue ◽  
Cell Interactions ◽  
Computerized Image Analysis ◽  
Cell Cell

scholarly journals Digital confocal microscopy allows measurement and three-dimensional multiple spectral reconstruction of Neisseria gonorrhoeae/epithelial cell interactions in the human fallopian tube organ culture model.

1994 ◽  
Vol 42 (3) ◽  
pp. 297-306 ◽  
Author(s):  
G L Gorby
Keyword(s):  
Image Analysis ◽  
Confocal Microscopy ◽  
Neisseria Gonorrhoeae ◽  
Organ Culture ◽  
Fallopian Tube ◽  
Three Dimensional ◽  
Target Tissue ◽  
Cell Nuclei ◽  
Computerized Image Analysis ◽  
Human Fallopian Tube

A strategy for measuring Neisseria gonorrhoeae attachment and invasion in the human Fallopian tube organ culture (FTOC) model via computerized image analysis (CIA) combined with "digital" confocal microscopy (DCM) was tested. DCM on serial image stacks of fluorescent latex beads reduced out-of-focus light propagation in the Z-axis (p < 0.005) and improved the shape factor of lateral three-dimensional reconstructions of the beads (p < 0.001). Sections of tissue infected for 44 hr with piliated, Opa+ gonococci were stained with fluorescein-labeled monoclonal anti-gonococcal antibodies, rhodamine-labeled phalloidin, and Hoechst 33342. Serial images collected at identical focal planes for each fluorochrome were subjected to DCM. Epithelial cytoplasmic regions of interest defined by rhodamine-stained actin were superimposed on the corresponding fluorescein-stained and Hoechst-stained images. Fluorescent objects defined by gray-scale threshold were measured by computerized image analysis using different border treatments to differentiate attached from intracellular gonococci or count cell nuclei. Compared with raw images, measurement of DCM images was less dependent on threshold choice (p < 0.05). DCM augments conventional microscopy in removing out-of-focus light from fluorescent images, in reconstructing three-dimensional images, and in quantitatively differentiating extracellular from intracellular gonococci in a natural target tissue.

Cell-cell interactions in diabetic neuropathy

2007 ◽  
Vol 2 (S 1) ◽  
Author(s):  
I Lukic ◽  
S Stoyanov ◽  
A Erhardt ◽  
P Nawroth ◽  
A Bierhaus
Keyword(s):  
Diabetic Neuropathy ◽  
Cell Interactions ◽  
Cell Cell

Application of Image Analysis System for Study of Activated Sludge Flocs

1986 ◽  
Vol 21 (1) ◽  
pp. 130-140 ◽  
Author(s):  
Da-hong Li ◽  
J. J. Ganczarczyk
Keyword(s):  
Image Analysis ◽  
Activated Sludge ◽  
Statistical Processing ◽  
Equivalent Diameter ◽  
Image Analysis System ◽  
Sectional Area ◽  
Cross Sectional ◽  
Computerized Image Analysis ◽  
Sludge Flocs ◽  
Analysis System

Abstract The computerized image analysis system has been successfully used for determination and statistical processing of the following geometric characteristics of activated sludge flocs: longest dimension, breadth, equivalent diameter, cross-sectional area, perimeter, elongation, and circularity. These parameters could be effectively and precisely determined by the system applied. In addition, the studied method, as compared to direct microscope observation and photography floc-sizing methods, was found to be more accurate, less time-consuming, and less dependent on the investigators.

scholarly journals Testicular organoids to study cell–cell interactions in the mammalian testis

Andrology ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 835-841 ◽  
Author(s):  
S. Sakib ◽  
T. Goldsmith ◽  
A. Voigt ◽  
I. Dobrinski
Keyword(s):  
Cell Interactions ◽  
Mammalian Testis ◽  
Cell Cell
Sign in / Sign up

Export Citation Format

Share Document