Multiplexed fluorescence detection for point-of-care

2012 ◽  
Author(s):  
Li Shen ◽  
Mike Ratterman ◽  
Tyler Stites ◽  
David Klotzkin ◽  
Ian Papautsky
Keyword(s):  
Fluorescence Detection ◽  
Point Of Care

scholarly journals A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications

2018 ◽  
Vol 117 ◽  
pp. 153-160 ◽  
Author(s):  
Uwadiae Obahiagbon ◽  
Joseph T. Smith ◽  
Meilin Zhu ◽  
Benjamin A. Katchman ◽  
Hany Arafa ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Point Of Care ◽  
Low Cost

Silicon thin film photodetectors for multi-channel fluorescence detection in a microfluidic point-of-care testing device

2016 ◽  
Vol 213 (7) ◽  
pp. 1686-1696 ◽  
Author(s):  
Marcel Berner ◽  
Ralph Rothemund ◽  
Sergej Vollmer ◽  
Markus Schubert ◽  
Urs Hilbig ◽  
...  
Keyword(s):  
Thin Film ◽  
Fluorescence Detection ◽  
Point Of Care ◽  
Testing Device ◽  
Point Of Care Testing ◽  
Silicon Thin Film

scholarly journals Transdermal fluorescence detection of a dual fluorophore system for noninvasive point-of-care gastrointestinal permeability measurement

2019 ◽  
Vol 10 (10) ◽  
pp. 5103
Author(s):  
Richard B. Dorshow ◽  
J. R. Johnson ◽  
Martin P. Debreczeny ◽  
I. Rochelle Riley ◽  
Jeng-Jong Shieh ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Point Of Care ◽  
Permeability Measurement ◽  
Gastrointestinal Permeability

scholarly journals Cleavable hairpin beacon-enhanced fluorescence detection of nucleic acid isothermal amplification and smartphone-based readout

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiong Ding ◽  
Kun Yin ◽  
Ziyue Li ◽  
Vikram Pandian ◽  
Joan A. Smyth ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Detection ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Lamp Assay ◽  
Dna Amplification ◽  
Isothermal Amplification ◽  
Specific Method ◽  
Enhanced Fluorescence ◽  
Resource Limited

Abstract Fluorescence detection of nucleic acid isothermal amplification utilizing energy-transfer-tagged oligonucleotide probes provides a highly sensitive and specific method for pathogen detection. However, currently available probes suffer from relatively weak fluorescence signals and are not suitable for simple, affordable smartphone-based detection at the point of care. Here, we present a cleavable hairpin beacon (CHB)-enhanced fluorescence detection for isothermal amplification assay. The CHB probe is a single fluorophore-tagged hairpin oligonucleotide with five continuous ribonucleotides which can be cleaved by the ribonuclease to specifically initiate DNA amplification and generate strong fluorescence signals. By coupling with loop-mediated isothermal amplification (LAMP), the CHB probe could detect Borrelia burgdorferi (B. burgdorferi) recA gene with a sensitivity of 100 copies within 25 min and generated stronger specific fluorescence signals which were easily read and analysed by our programmed smartphone. Also, this CHB-enhanced LAMP (CHB-LAMP) assay was successfully demonstrated to detect B. burgdorferi DNA extracted from tick species, showing comparable results to real-time PCR assay. In addition, our CHB probe was compatible with other isothermal amplifications, such as isothermal multiple-self-matching-initiated amplification (IMSA). Therefore, CHB-enhanced fluorescence detection is anticipated to facilitate the development of simple, sensitive smartphone-based point-of-care pathogen diagnostics in resource-limited settings.

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