Performance evaluation of point-spread function engineering to reduce the impact of depth-induced aberrations on extended depth-of-field microscopy

2012 ◽  
Author(s):  
Shuai Yuan ◽  
Chrysanthe Preza
Keyword(s):  
Performance Evaluation ◽  
Point Spread Function ◽  
Depth Of Field ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering ◽  
Extended Depth Of Field ◽  
The Impact

scholarly journals High-fidelity structured illumination microscopy by point-spread-function engineering

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gang Wen ◽  
Simin Li ◽  
Linbo Wang ◽  
Xiaohu Chen ◽  
Zhenglong Sun ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
High Fidelity ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Imaging Tool ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

AbstractStructured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

Point spread function engineering with multiphoton SPIFI

2016 ◽  
Author(s):  
Keith A. Wernsing ◽  
Jeffrey J. Field ◽  
Scott R. Domingue ◽  
Alyssa M. Allende-Motz ◽  
Keith F. DeLuca ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

scholarly journals Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method

2019 ◽  
Vol 12 (04) ◽  
pp. 1930005 ◽  
Author(s):  
Hexiang He ◽  
Xiangsheng Xie ◽  
Yikun Liu ◽  
Haowen Liang ◽  
Jianying Zhou
Keyword(s):  
Optical Imaging ◽  
Point Spread Function ◽  
Optical Memory ◽  
Ease Of Use ◽  
Large Field ◽  
Depth Of Field ◽  
Deconvolution Method ◽  
Scattering Medium ◽  
Point Spread ◽  
Spread Function

Visual perception of humans penetrating turbid medium is hampered by scattering. Various techniques have been prompted recently to recover optical imaging through turbid materials. Among them, speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality, robustness, ease-of-use, and ease-of-integration. By exploiting the point spread function (PSF) of the scattering system, large Field-of-View, extended Depth-of-Field, noninvasiveness and spectral resoluation are now available as successful solutions for high quality and multifunctional image reconstruction. In this paper, we review the progress of imaging through a scattering medium based on deconvolution method, including the principle, the breakthrough of the limitation of the optical memory effect, the improvement of the deconvolution algorithm and innovative applications.

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