scholarly journals Auto-luminescent genetically encoded ratiometric indicator for real-time Ca2+imaging at the single cell level

2011 ◽  
Author(s):  
Kenta Saito ◽  
Kentaro Kobayashi ◽  
Takeharu Nagai
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Cell Level

Measurement of Molecular Mobility in Mammalian Cells

2004 ◽  
Author(s):  
Alptekin Aksan ◽  
Mehmet Toner
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Chemical Reaction ◽  
Molecular Mobility ◽  
Mammalian Cells ◽  
Free Water ◽  
Single Cell Level ◽  
Extracellular Water ◽  
Cell Level

Preservation of mammalian cells requires establishing a reversible stasis condition by reducing the intra/extracellular molecular mobility ensuring reduced chemical reaction and deterioration rates. Molecular mobility may be reduced by various techniques. For example, in cryopreservation, mobility within and surrounding the cell is reduced through freezing the free water that constitutes 70–90% of the cell’s composition. In dried-state preservation applied successfully to preserve seeds, pharmacological materials and foodstuff (mimicking the anhydrobiosis phenomenon seen in nature), reduction in molecular mobility is established by removing intra/extracellular water. Certain carbohydrates (such as trehalose and sucrose) can be artificially uploaded into mammalian cells to replace the removed water and to form an intra/extracellular glass. In this research, a fluorescent rotor is utilized to determine the changes in intracellular molecular mobility during carbohydrate uploading of mammalian cells. It was shown that using this technique, it is feasible to make real-time mobility measurements at a single cell level.

scholarly journals Method for real-time monitoring of protein degradation at the single cell level

BioTechniques ◽  
2007 ◽  
Vol 42 (4) ◽  
pp. 446-450 ◽  
Author(s):  
Lijuan Zhang ◽  
Nadya G. Gurskaya ◽  
Ekaterina M. Merzlyak ◽  
Dmitry B. Staroverov ◽  
Nikolay N. Mudrik ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level

Reporter gene expression at single-cell level characterized with real-time RT-PCR, chemiluminescence, fluorescence, and electrochemical imaging

FEBS Letters ◽  
2010 ◽  
Vol 584 (18) ◽  
pp. 4000-4008 ◽  
Author(s):  
Hitoshi Shiku ◽  
Daisuke Okazaki ◽  
Junya Suzuki ◽  
Yasufumi Takahashi ◽  
Tatsuya Murata ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Real Time ◽  
Reporter Gene ◽  
Reporter Gene Expression ◽  
Single Cell Level ◽  
Rt Pcr ◽  
Cell Level ◽  
Electrochemical Imaging

scholarly journals Toward an understanding of immune cell sociology: real-time monitoring of cytokine secretion at the single-cell level

IUBMB Life ◽  
2012 ◽  
Vol 65 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Yoshitaka Shirasaki ◽  
Mai Yamagishi ◽  
Nanako Shimura ◽  
Atsushi Hijikata ◽  
Osamu Ohara
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Immune Cell ◽  
Cytokine Secretion ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level

Controlling gas/liquid exchange using microfluidics for real-time monitoring of flagellar length in living Chlamydomonas at the single-cell level

Lab on a Chip ◽  
2012 ◽  
Vol 12 (21) ◽  
pp. 4516 ◽  
Author(s):  
Xiaoni Ai ◽  
Qionglin Liang ◽  
Minna Luo ◽  
Kai Zhang ◽  
Junmin Pan ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level
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