scholarly journals Application of a new high-speed magnetic deformable mirror for in-vivo retinal imaging

2011 ◽  
Author(s):  
Sandra E. Balderas-Mata ◽  
Steven M. Jones ◽  
Robert J. Zawadzki ◽  
John S. Werner
Keyword(s):  
High Speed ◽  
Retinal Imaging ◽  
Deformable Mirror

scholarly journals In vivo retinal imaging for fixational eye motion detection using a high-speed digital micromirror device (DMD)-based ophthalmoscope

2018 ◽  
Vol 9 (2) ◽  
pp. 591 ◽  
Author(s):  
Kari V. Vienola ◽  
Mathi Damodaran ◽  
Boy Braaf ◽  
Koenraad A. Vermeer ◽  
Johannes F. de Boer
Keyword(s):  
Motion Detection ◽  
High Speed ◽  
Retinal Imaging ◽  
Digital Micromirror Device ◽  
Eye Motion

Two deformable mirror adaptive optics system for in vivo retinal imaging with optical coherence tomography

2006 ◽  
Author(s):  
Robert J. Zawadzki ◽  
Stacey S. Choi ◽  
John S. Werner ◽  
Steven M. Jones ◽  
Diana Chen ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Adaptive Optics ◽  
Retinal Imaging ◽  
Deformable Mirror ◽  
Optical Coherence ◽  
Adaptive Optics System

scholarly journals Adaptive-optics optical coherence tomography for high-resolution and high-speed in vivo retinal imaging

2005 ◽  
Author(s):  
Robert J. Zawadzki ◽  
Stacey Choi ◽  
Sophie Laut ◽  
John S. Werner ◽  
Steven M. Jones ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Adaptive Optics ◽  
High Speed ◽  
Retinal Imaging ◽  
Optical Coherence

Optimization of line-field spectral domain optical coherence tomography for in vivo high-speed 3D retinal imaging

2007 ◽  
Author(s):  
Yoshifumi Nakamura ◽  
Shuichi Makita ◽  
Masahiro Yamanari ◽  
Yoshiaki Yasuno ◽  
Masahide Itoh ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Speed ◽  
Retinal Imaging ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Line Field

scholarly journals Deformable mirror-based two-photon microscopy for axial mammalian brain imaging

2019 ◽  
Author(s):  
Alba Peinado ◽  
Eduardo Bendek ◽  
Sae Yokoyama ◽  
Kira E. Poskanzer
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Brain Slices ◽  
Axial Position ◽  
Mammalian Brain ◽  
Deformable Mirror ◽  
Radius Of Curvature ◽  
Step Size ◽  
Two Photon

AbstractThis work presents the design and implementation of an enhanced version of a traditional two-photon (2P) microscope with the addition of high-speed axial scanning for live mammalian brain imaging. Our implementation utilizes a deformable mirror (DM) that can rapidly apply different defocus shapes to manipulate the laser beam divergence and consequently control the axial position of the beam focus in the sample. We provide a mathematical model describing the DM curvature, then experimentally characterize the radius of curvature as well as the Zernike terms of the DM surface for a given set of defocuses. A description of the optical setup of the 2P microscope is detailed. We conduct a thorough calibration of the system, determining the point spread function, the total scanning range, the axial step size, and the intensity curvature as a function of depth. Finally, the instrument is used for imaging different neurobiological samples, including fixed brain slices and in vivo mouse cerebral cortex.

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

scholarly journals Correction-free remotely scanned two-photon in vivo mouse retinal imaging

2016 ◽  
Vol 5 (1) ◽  
pp. e16007-e16007 ◽  
Author(s):  
Adi Schejter Bar-Noam ◽  
Nairouz Farah ◽  
Shy Shoham
Keyword(s):  
Retinal Imaging ◽  
Two Photon

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

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