In vivo reconstruction of NIR FRET using full-field time resolved optical tomography

2011 ◽  
Author(s):  
Vivek Venugopal ◽  
Jin Chen ◽  
Margarida Barroso ◽  
Xavier Intes
Keyword(s):  
Optical Tomography ◽  
Time Resolved ◽  
Full Field

Time-resolved diffuse optical tomography and its application to in vitro and in vivo imaging

2007 ◽  
Vol 12 (6) ◽  
pp. 062107 ◽  
Author(s):  
Huijuan Zhao ◽  
Feng Gao ◽  
Yukari Tanikawa ◽  
Yukio Yamada
Keyword(s):  
In Vivo Imaging ◽  
Optical Tomography ◽  
Diffuse Optical Tomography ◽  
Time Resolved

B219 In vivo Time-resolved Diffuse Optical Tomography of Human Forearm under the Handgrip Exercise

2008 ◽  
Vol 2008.19 (0) ◽  
pp. 75-76
Author(s):  
Yukari TANIKAWA ◽  
Feng Gao ◽  
Yasuaki MIYAZAKI ◽  
Naoyuki IWATA ◽  
Yasuo NAKAMURA ◽  
...  
Keyword(s):  
Optical Tomography ◽  
Diffuse Optical Tomography ◽  
Handgrip Exercise ◽  
Time Resolved ◽  
Human Forearm

scholarly journals In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes

2018 ◽  
Author(s):  
Sez-Jade Chen ◽  
Nattawut Sinsuebphon ◽  
Alena Rudkouskaya ◽  
Margarida Barroso ◽  
Xavier Intes ◽  
...  
Keyword(s):  
Near Infrared ◽  
Specific Binding ◽  
Optical Tomography ◽  
Dynamic Imaging ◽  
Model Systems ◽  
Computationally Efficient ◽  
Time Resolved ◽  
Phasor Analysis

1AbstractWe introduce a simple new approach for time-resolved multiplexed analysis of complex systems using near-infrared (NIR) dyes, applicable to in vitro and in vivo studies. We first show that fast and precise in vitro quantification of NIR fluorophores lifetime and stoichiometry can be done using phasor analysis, a computationally efficient and user-friendly representation of complex fluorescence intensity decays obtained with pulsed laser excitation. We apply this approach to the study of binding equilibria by Förster resonant energy transfer (FRET), using two different model systems: primary/secondary antibody binding in vitro and ligand/receptor binding in cell cultures. We then extend our demonstration to dynamic imaging of the pharmacokinetics of transferrin binding to the transferrin receptor in live mice, elucidating the kinetic of differential transferrin accumulation in specific organs, straightforwardly differentiating specific from non-specific binding. Our method, implemented in a freely-available software package, has all the advantages of time-resolved NIR imaging, including better tissue penetration and background-free imaging, but simplifies and considerably speeds up data processing and interpretation, while remaining quantitative. These advances make this method attractive and of broad applicability for in vitro and in vivo molecular imaging, and could be extended to applications as diverse as image guided-surgery or optical tomography.

Short-Pulse Laser-Based System for Detection of Tumors: Administration of Gold Nanoparticles Enhances Contrast

2011 ◽  
Author(s):  
A. Y. Sajjadi ◽  
A. Suratkar ◽  
K. Mitra ◽  
M. S. Grace
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Detection ◽  
Short Pulse ◽  
Optical Tomography ◽  
Pulsed Laser ◽  
Mouse Tissue ◽  
Time Resolved ◽  
Tomography System ◽  
Short Pulse Laser

The objective of this paper is to demonstrate the use of gold nanoparticles, which accumulate in tumors due to the leakiness of tumor vasculature, as contrast agents for enhanced imaging in a time-resolved optical tomography system using short-pulse lasers for skin cancer detection. Mouse tissue was analyzed in vivo by optical coherence tomography during exposure to the beam of either a 514nm, 200ps pulsed laser or a 776nm, 1.3ps pulsed laser. It was found that addition of spherical gold nanoparticles, either by injection under the skin or by intravenous administration, decreased total reflection intensity and broadened the temporal profile of reflected photons. Together, these results show that gold nanoparticles tuned to the wavelength of the laser can enhance contrast between labeled and unlabeled tissues, and suggest that gold nanoparticles may enhance the sensitivity and resolution of laser-based cancer detection and therapeutic treatment.

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

scholarly journals Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)3]2+ In Vivo and In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 485
Author(s):  
Veronika Huntosova ◽  
Denis Horvath ◽  
Robert Seliga ◽  
Georges Wagnieres
Keyword(s):  
Oxidative Stress ◽  
Tissue Oxygenation ◽  
Intact Cell ◽  
Molecular Probes ◽  
Stress Factors ◽  
Time Resolved ◽  
Invasive Approach ◽  
Oxygen Detection

Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive approach to estimate tissue and cell oxygenation in vivo and in vitro. We optimized the evaluation of oxygen detection in vivo by [Ru(Phen)3]2+ in the chicken embryo chorioallantoic membrane model. Its luminescence lifetimes measured in the CAM were analyzed through hierarchical clustering. The detection of the tissue oxygenation at the oxidative stress conditions is still challenging. We applied simultaneous time-resolved recording of the mitochondrial probe MitoTrackerTM OrangeCMTMRos fluorescence and [Ru(Phen)3]2+ phosphorescence imaging in the intact cell without affecting the sensitivities of these molecular probes. [Ru(Phen)3]2+ was demonstrated to be suitable for in vitro detection of oxygen under various stress factors that mimic oxidative stress: other molecular sensors, H2O2, and curcumin-mediated photodynamic therapy in glioma cancer cells. Low phototoxicities of the molecular probes were finally observed. Our study offers a high potential for the application and generalization of tissue oxygenation as an innovative approach based on the similarities between interdependent biological influences. It is particularly suitable for therapeutic approaches targeting metabolic alterations as well as oxygen, glucose, or lipid deprivation.

scholarly journals A Versatile Setup for Time-Resolved Functional Near Infrared Spectroscopy Based on Fast-Gated Single-Photon Avalanche Diode and on Four-Wave Mixing Laser

2019 ◽  
Vol 9 (11) ◽  
pp. 2366 ◽  
Author(s):  
Laura Di Sieno ◽  
Alberto Dalla Mora ◽  
Alessandro Torricelli ◽  
Lorenzo Spinelli ◽  
Rebecca Re ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Single Photon ◽  
Wave Mixing ◽  
Functional Near Infrared Spectroscopy ◽  
Time Resolved ◽  
Diffusive Medium ◽  
Spectroscopy System

In this paper, a time-domain fast gated near-infrared spectroscopy system is presented. The system is composed of a fiber-based laser providing two pulsed sources and two fast gated detectors. The system is characterized on phantoms and was tested in vivo, showing how the gating approach can improve the contrast and contrast-to-noise-ratio for detection of absorption perturbation inside a diffusive medium, regardless of source-detector separation.

A self-normalized full time-resolved scheme for fluorescence diffuse optical tomography

2008 ◽  
Author(s):  
Feng Gao ◽  
Limin Zhang ◽  
Huiyuan He ◽  
Huijuan Zhao ◽  
Jingying Jiang ◽  
...  
Keyword(s):  
Optical Tomography ◽  
Diffuse Optical Tomography ◽  
Full Time ◽  
Time Resolved ◽  
Fluorescence Diffuse Optical Tomography

In vivo high-contrast visualization of upconversion nanoparticle labeled virus using time-resolved approach

Nano Today ◽  
2021 ◽  
Vol 40 ◽  
pp. 101264
Author(s):  
Gaoju Pang ◽  
Yingying Zhang ◽  
Xiaoyong Wang ◽  
Huizhuo Pan ◽  
Xinyu Zhang ◽  
...  
Keyword(s):  
High Contrast ◽  
Time Resolved
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