A unified approach for high throughput analysis of real-time biomolecular interactions in surface plasmon resonance and fluorescence imaging

2011 ◽  
Author(s):  
Catalin Fetita ◽  
Nicolas François ◽  
Françoise Prêteux ◽  
Hervé Delacroix
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Biomolecular Interactions ◽  
Unified Approach ◽  
High Throughput Analysis ◽  
Throughput Analysis

Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

Lab on a Chip ◽  
2010 ◽  
Vol 10 (8) ◽  
pp. 986 ◽  
Author(s):  
Ganeshram Krishnamoorthy ◽  
Edwin T. Carlen ◽  
Johan G. Bomer ◽  
Daniël Wijnperlé ◽  
Hans L. deBoer ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals Surface plasmon resonance: principles, methods and applications in biomedical sciences

2003 ◽  
Vol 17 (2-3) ◽  
pp. 255-273 ◽  
Author(s):  
Patrick Englebienne ◽  
Anne Van Hoonacker ◽  
Michel Verhas
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Metal Surface ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Colloidal Gold ◽  
Biomedical Applications ◽  
Clinical Chemistry ◽  
Label Free

Surface plasmon resonance (SPR) is a phenomenon occuring at metal surfaces (typically gold and silver) when an incident light beam strikes the surface at a particular angle. Depending on the thickness of a molecular layer at the metal surface, the SPR phenomenon results in a graded reduction in intensity of the reflected light. Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands. The last ten years have seen a tremendous development of SPR use in biomedical applications. The technique is applied not only to the measurement in real-time of the kinetics of ligand–receptor interactions and to the screening of lead compounds in the pharmaceutical industry, but also to the measurement of DNA hybridization, enzyme–substrate interactions, in polyclonal antibody characterization, epitope mapping, protein conformation studies and label-free immunoassays. Conventional SPR is applied in specialized biosensing instruments. These instruments use expensive sensor chips of limited reuse capacity and require complex chemistry for ligand or protein immobilization. Our laboratory has successfully applied SPR with colloidal gold particles in buffered solution. This application offers many advantages over conventional SPR. The support is cheap, easily synthesized, and can be coated with various proteins or protein–ligand complexes by charge adsorption. With colloidal gold, the SPR phenomenon can be monitored in any UV-vis spectrophotometer. For high‒throughput applications, we have adapted the technology in an automated clinical chemistry analyzer. This simple technology finds application in label-free quantitative immunoassay techniques for proteins and small analytes, in conformational studies with proteins as well as in the real-time association-dissociation measurements of receptor–ligand interactions, for high-throughput screening and lead optimization.

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