Accelerated binding kinetics by surface acoustic waves (SAW) micromixing in surface plasmon resonance (SPR) system for biodetection

2011 ◽  
Author(s):  
Alan Renaudin ◽  
Vincent Chabot ◽  
Etienne Grondin ◽  
Vincent Aimez ◽  
Paul G. Charette
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Binding Kinetics

Integrated active mixing and biosensing using surface acoustic waves (SAW) and surface plasmon resonance (SPR) on a common substrate

Lab on a Chip ◽  
2010 ◽  
Vol 10 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Alan Renaudin ◽  
Vincent Chabot ◽  
Etienne Grondin ◽  
Vincent Aimez ◽  
Paul G. Charette
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Common Substrate ◽  
Active Mixing

scholarly journals Binding Kinetics of Methylene Blue on Monolayer Graphene Investigated by Multiparameter Surface Plasmon Resonance

ACS Omega ◽  
2018 ◽  
Vol 3 (7) ◽  
pp. 7133-7140 ◽  
Author(s):  
Nur Selin Kaya ◽  
Anur Yadav ◽  
Michel Wehrhold ◽  
Laura Zuccaro ◽  
Kannan Balasubramanian
Keyword(s):  
Methylene Blue ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Kinetics ◽  
Monolayer Graphene ◽  
Kinetics Of

scholarly journals Sensor Chip Using a Plasma-polymerized Film for Surface Plasmon Resonance Biosensors. Reliable Analysis of Binding Kinetics.

2000 ◽  
Vol 16 (4) ◽  
pp. 347-348 ◽  
Author(s):  
Hitoshi MUGURUMA ◽  
Ryohei NAGATA ◽  
Runa NAKAMURA ◽  
Kimiharu SATO ◽  
Shigeru UCHIYAMA ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Kinetics ◽  
Sensor Chip ◽  
Reliable Analysis

scholarly journals Molecular flexibility of DNA as a key determinant of RAD51 recruitment

2018 ◽  
Author(s):  
Federico Paoletti ◽  
Afaf El-Sagheer ◽  
Jun Allard ◽  
Tom Brown ◽  
Omer Dushek ◽  
...  
Keyword(s):  
Dna Damage ◽  
Surface Plasmon Resonance ◽  
Homologous Recombination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Genome Stability ◽  
General Framework ◽  
Binding Kinetics ◽  
Double Stranded Dna ◽  
Conceptual Question

AbstractThe timely activation of homologous recombination is essential for the maintenance of genome stability, in which the RAD51 recombinase plays a central role. Biochemically, human RAD51 polymerises faster on single-stranded DNA (ssDNA) compared to double-stranded DNA (dsDNA), raising a key conceptual question: how does it discriminate between them? In this study, we tackled this problem by systematically assessing RAD51 binding kinetics on ssDNA and dsDNA differing in length and flexibility using surface plasmon resonance. By fitting detailed polymerisation models informed by our experimental datasets, we show that RAD51 is a mechano-sensor that exhibits a larger polymerisation rate constant on flexible ssDNA compared to rigid ssDNA or dsDNA. This model presents a new general framework suggesting that the flexibility of DNA, which may increase locally as a result of DNA damage, plays an important role in rapidly recruiting repair factors that multimerise at sites of DNA damage.

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