Differentiating human cervical dysplastic and normal tissue through wavelet domain characterization of intrinsic fluorescence

T2*-weighted MRI of transient oxygen challenge (OC) showed exaggerated OC percent changes in the ischemic tissue at risk compared to normal tissue. One ambiguity is that regions with high vascular density also showed exaggerated OC percent changes. This study explored time-to-peak (TTP) of the OC percent changes to improve the utility of T2*-weighted OC MRI. Experiments were performed longitudinally at 30 min, 150 min and 24 h after transient (60-min) stroke in rats. Ischemic core, normal, and mismatch tissue were classified pixel-by-pixel based on apparent diffusion coefficient and cerebral blood flow. Major findings were: (i) Delayed OC TTP was localized to and corresponded well with the perfusion-diffusion mismatch. (ii) By contrast, the exaggerated OC percent changes were less localized, with changes not only in the at-risk tissue but also in some areas of the contralesional hemisphere with venous vessel origins. (iii) The OC time-course of the mismatch tissue was biphasic, with a faster initial increase followed by a slower increase. (iv) At-risk tissue with delayed TTP and exaggerated OC was normal after reperfusion and the at-risk tissue was mostly (83 ± 18%) rescued by reperfusion as indicated by normal 24-h T2. OC TTP offers unique information toward better characterization of at-risk tissue in ischemic stroke.

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Image deconvolution using a characterization of sharp images in wavelet domain

Applied and Computational Harmonic Analysis ◽

10.1016/j.acha.2011.09.006 ◽

2012 ◽

Vol 32 (2) ◽

pp. 295-304 ◽

Cited By ~ 15

Author(s):

Hui Ji ◽

Jia Li ◽

Zuowei Shen ◽

Kang Wang

Keyword(s):

Image Deconvolution ◽

Wavelet Domain

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Characterization of the Human Tumor and Normal Tissue Reactivity of the KS1/4 Monoclonal Antibody

Hybridoma ◽

10.1089/hyb.1988.7.407 ◽

1988 ◽

Vol 7 (4) ◽

pp. 407-415 ◽

Cited By ~ 34

Author(s):

THOMAS F. BUMOL ◽

PHILIP MARDER ◽

SONJA V. DeHERDT ◽

MICHAEL J. BOROWITZ ◽

LYNN D. APELGREN

Keyword(s):

Monoclonal Antibody ◽

Normal Tissue ◽

Human Tumor ◽

Tissue Reactivity

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Characterization of cancer and normal tissue fluorescence through wavelet transform and singular value decomposition

10.1117/12.768912 ◽

2008 ◽

Cited By ~ 2

Author(s):

Anita H. Gharekhan ◽

Nrusingh C. Biswal ◽

Sharad Gupta ◽

Asima Pradhan ◽

M. B. Sureshkumar ◽

...

Keyword(s):

Wavelet Transform ◽

Singular Value Decomposition ◽

Normal Tissue ◽

Singular Value ◽

Tissue Fluorescence ◽

Value Decomposition

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Characterizing the Intrinsic Fluorescence Properties of Historical Painting Materials: The Case Study of a Sixteenth-Century Mesoamerican Manuscript

Applied Spectroscopy ◽

10.1177/0003702817747276 ◽

2017 ◽

Vol 72 (4) ◽

pp. 573-583 ◽

Cited By ~ 4

Author(s):

Fabien Pottier ◽

Anne Michelin ◽

Christine Andraud ◽

Fabrice Goubard ◽

Bertrand Lavédrine

Keyword(s):

Sixteenth Century ◽

Fluorescence Spectroscopy ◽

Paint Layer ◽

Intrinsic Fluorescence ◽

16Th Century ◽

Fluorescent Properties ◽

Painting Materials ◽

Intrinsic Emission

Ultraviolet visible (UV–Vis) fluorescence spectroscopy is widely used to study polychrome objects and can help to identify the nature of certain materials when they present specific fluorescent properties. However, given the complexity of the stratified and heterogeneous materials under study, the characterization of an intrinsic fluorescence related to a given constituent (a pigment or a binder composing a paint layer for example) is not straightforward, and the recorded raw data need to be corrected for a number of effects that can influence the detected spectral distribution. The application of standard correction procedures to experimental fluorescence data gathered on the polychromatic surface of the Codex Borbonicus, a 16th-century Aztec manuscript, is described. The results are confronted to an alternate new methodology that is based on the hypothesis of transparent non-scattering paint layers. This second approach allows to establish more clearly the material origin of the detected emission and to discriminate apparent fluorescence (emitted by the substrate and transmitted through the paint layers) from actual intrinsic emission generated by the coloring materials under study. The results show that most of the various emission profiles detected in the paint layers of the manuscript actually originate from a unique fluorophore (composing the substrate) and should not be used to characterize the coloring materials.

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