Multiparameter label-free flow cytometry using multiplex coherent anti-Stokes Raman scattering (MCARS) with biological applications

2011 ◽  
Author(s):  
Charles H. Camp, Jr. ◽  
Siva Yegnanarayanan ◽  
Ali A. Eftekhar ◽  
Ali Adibi
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Free Flow ◽  
Biological Applications ◽  
Label Free ◽  
Anti Stokes

Label-free flow cytometry using multiplex coherent anti-Stokes Raman scattering (MCARS) for the analysis of biological specimens

2011 ◽  
Vol 36 (12) ◽  
pp. 2309 ◽  
Author(s):  
Charles H. Camp, Jr. ◽  
Siva Yegnanarayanan ◽  
Ali A. Eftekhar ◽  
Ali Adibi
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Free Flow ◽  
Label Free ◽  
Anti Stokes

scholarly journals Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry

2009 ◽  
Vol 17 (25) ◽  
pp. 22879 ◽  
Author(s):  
Charles H. Camp Jr. ◽  
Siva Yegnanarayanan ◽  
Ali A. Eftekhar ◽  
Hamsa Sridhar ◽  
Ali Adibi
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Free Flow ◽  
Label Free ◽  
Anti Stokes

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy

2014 ◽  
pp. 189-201 ◽  
Author(s):  
Antti Isomäki ◽  
Tarvo Sillat ◽  
Mari Ainola ◽  
Mikko Liljeström ◽  
Yrjö T. Konttinen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Label Free ◽  
Anti Stokes

Application of machine learning-driven label-free flow cytometry to analyze T cell products

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S132-S133
Author(s):  
H. Ochiai ◽  
K. Teranishi ◽  
K. Toda ◽  
K. Sugimoto ◽  
S. Ota
Keyword(s):  
Machine Learning ◽  
Flow Cytometry ◽  
T Cell ◽  
Free Flow ◽  
Label Free

scholarly journals Coherent anti-Stokes Raman scattering for label-free biomedical imaging

2013 ◽  
Vol 15 (9) ◽  
pp. 094006 ◽  
Author(s):  
Imran I Patel ◽  
Christian Steuwe ◽  
Stefanie Reichelt ◽  
Sumeet Mahajan
Keyword(s):  
Raman Scattering ◽  
Biomedical Imaging ◽  
Label Free ◽  
Anti Stokes

Label-Free Imaging of Lipophilic Bioactive Molecules during Lipid Digestion by Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy

2010 ◽  
Vol 132 (24) ◽  
pp. 8433-8439 ◽  
Author(s):  
James P. R. Day ◽  
Gianluca Rago ◽  
Katrin F. Domke ◽  
Krassimir P. Velikov ◽  
Mischa Bonn
Keyword(s):  
Raman Scattering ◽  
Bioactive Molecules ◽  
Label Free ◽  
Lipid Digestion ◽  
Anti Stokes

scholarly journals Label-Free Fluctuation Spectroscopy Based on Coherent Anti-Stokes Raman Scattering from Bulk Water Molecules

ChemPhysChem ◽  
2016 ◽  
Vol 17 (7) ◽  
pp. 1025-1033 ◽  
Author(s):  
M. D. Rabasovic ◽  
E. Sisamakis ◽  
S. Wennmalm ◽  
J. Widengren
Keyword(s):  
Raman Scattering ◽  
Bulk Water ◽  
Water Molecules ◽  
Label Free ◽  
Anti Stokes

scholarly journals Stimulated Raman scattering flow cytometry for label-free single-particle analysis

Optica ◽  
2017 ◽  
Vol 4 (1) ◽  
pp. 103 ◽  
Author(s):  
Chi Zhang ◽  
Kai-Chih Huang ◽  
Bartek Rajwa ◽  
Junjie Li ◽  
Shiqi Yang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Single Particle ◽  
Stimulated Raman Scattering ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Label Free ◽  
Stimulated Raman
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