scholarly journals Assessment of tissue optical clearing as a function of glucose concentration using optical coherence tomography

2011 ◽  
Author(s):  
Narendran Sudheendran ◽  
Mohamed Mohamed ◽  
Mohamad G. Ghosn ◽  
Valery V. Tuchin ◽  
Kirill V. Larin
Keyword(s):  
Optical Coherence Tomography ◽  
Glucose Concentration ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Tissue Optical Clearing

scholarly journals ASSESSMENT OF TISSUE OPTICAL CLEARING AS A FUNCTION OF GLUCOSE CONCENTRATION USING OPTICAL COHERENCE TOMOGRAPHY

2010 ◽  
Vol 03 (03) ◽  
pp. 169-176 ◽  
Author(s):  
NARENDRAN SUDHEENDRAN ◽  
MOHAMED MOHAMED ◽  
MOHAMAD G. GHOSN ◽  
VALERY V. TUCHIN ◽  
KIRILL V. LARIN
Keyword(s):  
Optical Coherence Tomography ◽  
Glucose Concentration ◽  
Glucose Solution ◽  
Tissue Sample ◽  
Biological Tissues ◽  
Optical Coherence ◽  
Porcine Skin ◽  
Optical Clearing ◽  
Reflected Light ◽  
Tissue Optical Clearing

One of the major challenges in imaging biological tissues using optical techniques, such as optical coherence tomography (OCT), is the lack of light penetration due to highly turbid structures within the tissue. Optical clearing techniques enable the biological samples to be more optically homogeneous, allowing for deeper penetration of light into the tissue. This study investigates the effect of optical clearing utilizing various concentrations of glucose solution (10%, 30%, and 50%) on porcine skin. A gold-plated mirror was imaged beneath the tissue and percentage clearing was determined by monitoring the change in reflected light intensity from the mirror over time. The ratio of percentage clearing per tissue thickness for 10%, 30% and 50% glucose was determined to be 4.7 ±1.6% mm-1 (n = 6), 10.6 ±2.0% mm-1 (n = 7) and 21.8 ±2.2% mm-1 (n = 5), respectively. It was concluded that while higher glucose concentration has the highest optical clearing effect, a suitable concentration should be chosen for the purpose of clearing, considering the osmotic stress on the tissue sample.

Monitoring of the Process of Immersion Optical Clearing of Collagen Bundles Using Optical Coherence Tomography

2019 ◽  
Vol 127 (2) ◽  
pp. 359-367 ◽  
Author(s):  
M. E. Shvachkina ◽  
D. D. Yakovlev ◽  
E. N. Lazareva ◽  
A. B. Pravdin ◽  
D. A. Yakovlev
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Optical Clearing

scholarly journals Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents

2020 ◽  
Vol 245 (18) ◽  
pp. 1629-1636
Author(s):  
Ruiming Kong ◽  
Wenjuan Wu ◽  
Rui Qiu ◽  
Lei Gao ◽  
Fengxian Du ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Light Attenuation ◽  
Anterior Segment ◽  
Posterior Segment ◽  
Glycerol Solution ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Imaging Depth

Optical coherence tomography has become an indispensable diagnostic tool in ophthalmology for imaging the retina and the anterior segment of the eye. However, the imaging depth of optical coherence tomography is limited by light attenuation in tissues due to optical scattering and absorption. In this study of rabbit eye both ex vivo and in vivo, optical coherence tomography imaging depth of the anterior and posterior segments of the eye was extended by using optical clearing agents to reduce multiple scattering. The sclera, the iris, and the ciliary body were clearly visualized by direct application of glycerol at an incision on the conjunctiva, and the posterior boundary of sclera and even the deeper tissues were detected by submerging the posterior segment of eye in glycerol solution ex vivo or by retro-bulbar injection of glycerol in vivo. The ex vivo rabbit eyes recovered to their original state in 60 s after saline-wash treatment, and normal optical coherence tomography images of the posterior segment of the sample eyes proved the self-recovery of in vivo performance. Signal intensities of optical coherence tomography images obtained before and after glycerol treatment were compared to analysis of the effect of optical clearing. To the best of our knowledge, this is the first study for imaging depth extension of optical coherence tomography in both the anterior and posterior segments of eye by using optical clearing agents.

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