Integrated lab-on-a-chip: a combined sample preparation and PCR system as an ultrafast analytical tool for pathogen detection

2011 ◽  
Author(s):  
Holger Becker ◽  
Nadine Hlawatsch ◽  
Richard Klemm ◽  
Claudia Gärtner
Keyword(s):  
Sample Preparation ◽  
Pathogen Detection ◽  
Lab On A Chip ◽  
Analytical Tool

Manipulation of Clinical Materials Using AC Electrokinetics

2010 ◽  
Author(s):  
Mandy L. Y. Sin ◽  
Pak Kin Wong
Keyword(s):  
Experimental Data ◽  
Sample Preparation ◽  
Theoretical Prediction ◽  
Fluid Motion ◽  
Lab On A Chip ◽  
Clinical Samples ◽  
Electrochemical Reactions ◽  
Ac Electrokinetics ◽  
Wide Range ◽  
Electrothermal Flow

AC electrokinetics is a promising approach for sample preparation and reaction enhancement in lab-on-a-chip devices. However, relative little has been done on the electrokinetic manipulation of physiological fluids and buffers with similar properties, such as conductivity. Herein, electrokinetic manipulation of fluids with a wide range of conductivities has been studied as a function of voltage and frequency. AC electrothermal flow is determined to dominate the fluid motion when the applied frequency of the AC potential is above 100 kHz. Interestingly, experimental data deviate from theoretical prediction for fluids with high conductivities (> 1 Sm−1). The deviation can be understood by voltage modulated electrochemical reactions and should be accounted for when manipulating clinical materials with high conductivities. The study will provide useful in sights in designing lab-on-a-chip devices for manipulating clinical samples in the future.

Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review

2021 ◽  
Author(s):  
Mylena Lemes Cunha ◽  
Stella Schuster da Silva ◽  
Mateus Cassaboni Stracke ◽  
Dalila Luciola Zanette ◽  
Mateus Nóbrega Aoki ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Lab On A Chip

A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening

2019 ◽  
Vol 288 ◽  
pp. 171-179 ◽  
Author(s):  
K. Tsougeni ◽  
A.S. Kastania ◽  
G.D. Kaprou ◽  
Michael Eck ◽  
Gerhard Jobst ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Foodborne Pathogen ◽  
Lab On A Chip ◽  
Highly Efficient ◽  
Pathogen Screening

scholarly journals Proof of Concept for Recombinant Cellular Controls in Quantitative Molecular Pathogen Detection

2011 ◽  
Vol 77 (7) ◽  
pp. 2531-2533 ◽  
Author(s):  
Peter Rossmanith ◽  
Patrick Mester ◽  
Karin Frühwirth ◽  
Sabine Fuchs ◽  
Martin Wagner
Keyword(s):  
Listeria Monocytogenes ◽  
Sample Preparation ◽  
Quantitative Pcr ◽  
Pathogen Detection ◽  
Single Copy ◽  
Deletion Strain ◽  
Proof Of Concept ◽  
Targeted Deletion ◽  
Process Controls

ABSTRACTIn this study, we present the concept of internal sample process controls (ISPCs) to monitor the efficiency of an analytical chain using sample preparation and quantitative PCR (qPCR). A recombinantListeria monocytogenesΔprfA(targeted deletion) strain containing a competitive artificial single-copy genomic target was applied to naturally contaminated samples to demonstrate its analytical suitability as an ISPC.

Fully Automated Sample Preparation for Pathogen Detection Performed in a Microfluidic Cassette

2001 ◽  
pp. 670-672 ◽  
Author(s):  
M. T. Taylor ◽  
P. Belgrader ◽  
R. Joshi ◽  
G. A. Kintz ◽  
M. A. Northrup
Keyword(s):  
Sample Preparation ◽  
Pathogen Detection ◽  
Automated Sample Preparation

Rapid and fully automated bacterial pathogen detection on a centrifugal-microfluidic LabDisk using highly sensitive nested PCR with integrated sample preparation

Lab on a Chip ◽  
2015 ◽  
Vol 15 (18) ◽  
pp. 3749-3759 ◽  
Author(s):  
G. Czilwik ◽  
T. Messinger ◽  
O. Strohmeier ◽  
S. Wadle ◽  
F. von Stetten ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Nested Pcr ◽  
Pathogen Detection ◽  
Bacterial Pathogen ◽  
Highly Sensitive

Integrated PCR LabDisk and portable LabDisk-Player.

scholarly journals A robust, hand-powered, instrument-free sample preparation system for point-of-care pathogen detection

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fei Zhao ◽  
Eun Yeong Lee ◽  
Geun Su Noh ◽  
Jaehyup Shin ◽  
Huifang Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Sample Pretreatment ◽  
Sample Volume ◽  
Nucleic Acid Isolation ◽  
Point Of Care Diagnostics ◽  
Reaction Matrix

Abstract Here, we describe a simple, universal protocol for use in nucleic acid testing-based pathogen diagnostics, which requires only hand-powered sample preparation, including the processes of pathogen enrichment and nucleic acid isolation. The protocol uses low-cost amine-functionalized diatomaceous earth with a 1-μm Teflon filter as a reaction matrix in both stages of the process, using homobifunctional imidoesters. Using a simple syringe as a pump, the capture efficiency for a large sample volume (<50 mL) was enhanced by up to 98.3%, and the detection limit was 1 CFU/mL, 100-fold better than that of common commercial nucleic acid isolation kit. This protocol can also be combined with commercialized 96-well filter plates for robust sample preparation. Our proposed system is robust, simple, low-cost, universal, and rapid (taking <20 min), and it works regardless of the ambient environment and sample pretreatment, requiring no electricity or instruments. Its benefits include the simplicity of producing its components and its ease of operation, and it can be readily integrated with other assays for point-of-care diagnostics.

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