Quantitative analysis of live cells using digital holographic microscopy

2009 ◽  
Author(s):  
Tan Rongwei Lewis ◽  
Weijuan Qu ◽  
Oi Choo Chee ◽  
Vijay Raj Singh ◽  
Anand Asundi
Keyword(s):  
Quantitative Analysis ◽  
Digital Holographic Microscopy ◽  
Live Cells ◽  
Holographic Microscopy

Digital Holographic Microscopy

1999 ◽  
Vol 5 (S2) ◽  
pp. 362-363
Author(s):  
Tong Zhang ◽  
Ichirou Yamaguchi ◽  
Hywel Morgan
Keyword(s):  
Quantitative Analysis ◽  
Optical Microscopy ◽  
Digital Holography ◽  
Focal Depth ◽  
Phase Shifting ◽  
Digital Holographic Microscopy ◽  
Photographic Recording ◽  
Scanning Confocal Microscopy ◽  
Optical Scanning Holography ◽  
Holographic Microscopy

We applied phase-shifting digital holography to microscopy in this paper. At first lensless microscopy is proposed, in which no optical adjustment is necessary. Then, the method is applied to relax the limitation of focal depth in traditional optical microscopy. A theory for image formation and experimental verification using a few specimens are described.keywords: microscopy, digital holography, phase shiftingDue to the finite focal depth of an imaging lens, a limitation to normal optical microscopy-is that, only the 2-dimensional (2-D) information of an object can be obtained at one time. Besides, it is not convenient for quantitative analysis the observed image. Optical sectioning microscopy (OSM) and scanning confocal microscopy (SCM) which use opto-electronic detection have been proposed for quantitative analysis of a 3-D object. However, the former requires critical mechanical adjustment, while the latter uses timeconsuming mechanical 3-D scanning. Holographic microscopy can solve these problems because it can record 3-D information at one time. But, the chemical processing of holograms and the mechanical focusing at the reconstructed images cause more or less trouble. A 3-D imaging technique without use of photographic recording called optical scanning holography has recently been reported. However, there are also some trouble owing to the twin-image noise.

scholarly journals Quantitative analysis of platelets aggregates in 3D by digital holographic microscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 3556 ◽  
Author(s):  
Karim Zouaoui Boudejltia ◽  
Daniel Ribeiro de Sousa ◽  
Pierrick Uzureau ◽  
Catherine Yourassowsky ◽  
David Perez-Morga ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

Quantitative analysis of living cells by digital holographic microscopy

2009 ◽  
Author(s):  
Benjamin E. Reese ◽  
Robert E. Barnett ◽  
Mustapha Alaoui-Ismaili ◽  
Emil Habibi ◽  
Christopher J. Mann
Keyword(s):  
Quantitative Analysis ◽  
Living Cells ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

3D measurements of live cells via digital holographic microscopy and terahertz spectroscopy

2016 ◽  
Author(s):  
Jun Yong Park ◽  
Dorian Oser ◽  
Peter Iapozzuto ◽  
Sean Norbury ◽  
Supriya Mahajan ◽  
...  
Keyword(s):  
Terahertz Spectroscopy ◽  
Digital Holographic Microscopy ◽  
Live Cells ◽  
3D Measurements ◽  
Holographic Microscopy
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