A porous silicon based microarray for label-free optical detection of DNA hybridization

2010 ◽  
Author(s):  
Ilaria Rea ◽  
Giuseppe Coppola ◽  
Mariano Gioffrè ◽  
Edoardo De Tommasi ◽  
Ivo Rendina ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Dna Hybridization ◽  
Optical Detection ◽  
Label Free ◽  
Silicon Based

Optical detection of PNA/DNA hybridization in resonant porous silicon-based devices

2008 ◽  
Author(s):  
Lucia Rotiroti ◽  
Paolo Arcari ◽  
Annalisa Lamberti ◽  
Carmen Sanges ◽  
Edoardo De Tommasi ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Dna Hybridization ◽  
Optical Detection ◽  
Silicon Based

scholarly journals Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Food Industry

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Naama Massad-Ivanir ◽  
Giorgi Shtenberg ◽  
Nitzan Raz ◽  
Christel Gazenbeek ◽  
Dries Budding ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Real Time ◽  
Food Industry ◽  
Optical Detection ◽  
Silicon Based

Label-free optical detection of bacteria on a 1-D photonic crystal of porous silicon

2009 ◽  
Author(s):  
Chia-Chen Wu ◽  
Sara D. Alvarez ◽  
Camilla U. Rang ◽  
Lin Chao ◽  
Michael J. Sailor
Keyword(s):  
Photonic Crystal ◽  
Porous Silicon ◽  
Optical Detection ◽  
Label Free

Fabrication and characterization of a porous silicon based microarray for label-free optical monitoring of biomolecular interactions

2010 ◽  
Vol 107 (1) ◽  
pp. 014513 ◽  
Author(s):  
Ilaria Rea ◽  
Annalisa Lamberti ◽  
Ivo Rendina ◽  
Giuseppe Coppola ◽  
Mariano Gioffrè ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Biomolecular Interactions ◽  
Optical Monitoring ◽  
Label Free ◽  
Fabrication And Characterization ◽  
Silicon Based

scholarly journals Label-Free Optical Detection of Peptide Synthesis on a Porous Silicon Scaffold/Sensor

Langmuir ◽  
2008 ◽  
Vol 24 (6) ◽  
pp. 2908-2915 ◽  
Author(s):  
Patrick Furbert ◽  
Caiyan Lu ◽  
Nicholas Winograd ◽  
Lisa DeLouise
Keyword(s):  
Porous Silicon ◽  
Peptide Synthesis ◽  
Optical Detection ◽  
Label Free

Electrical Porous Silicon Microarray for dna Hybridization Detection

2003 ◽  
Vol 782 ◽  
Author(s):  
Marie Archer ◽  
Marc Christophersen ◽  
Philippe M. Fauchet ◽  
Deoram Persaud ◽  
Karl D. Hirschman
Keyword(s):  
Porous Silicon ◽  
Real Time ◽  
Dna Hybridization ◽  
Electrical Contacts ◽  
Macroporous Silicon ◽  
Label Free ◽  
Internal Surface Area ◽  
Free Standing ◽  
Sensing Applications ◽  
Label Free Detection

ABSTRACTThe sensitivity of Porous Silicon (PSi) to the presence of charged molecules and its large internal surface area represent two important properties that make this material and ideal candidate for electrical biosensor development. We have demonstrated the use of a macroporous silicon electrical sensor for label-free detection of DNA hybridization in real time as well as identification of organic solvents in liquid phase. Binding of DNA inside the PSi matrix induces a change in capacitance and conductance. Having demonstrated the suitability of macroporous silicon layers for real time detection of DNA hybridization on single devices, we have extended our findings to the fabrication of a microarray with individual device electrical addressing capabilities. On a crystalline p-type silicon wafer, process steps such as KOH etching and electrochemical dissolution are employed in selected regions to create a free-standing porous membrane for sensing applications. Individual electrical contacts are made on the front side of the wafer while the infiltration of the probe and target molecules is done from the back avoiding any direct interaction of the molecules with the contact sites. We will report on the design considerations of the electrical porous silicon array and the preliminary results obtained using synthetic DNA as a model molecule.

scholarly journals Porous Silicon-Based Aptasensors: The Next Generation of Label-Free Devices for Health Monitoring

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2216 ◽  
Author(s):  
Monica Terracciano ◽  
Ilaria Rea ◽  
Nicola Borbone ◽  
Rosalba Moretta ◽  
Giorgia Oliviero ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Porous Silicon ◽  
Limit Of Detection ◽  
Combinatorial Libraries ◽  
High Sensitivity ◽  
Label Free ◽  
Great Specificity ◽  
Silicon Based ◽  
Exponential Enrichment

Aptamers are artificial nucleic acid ligands identified and obtained from combinatorial libraries of synthetic nucleic acids through the in vitro process SELEX (systematic evolution of ligands by exponential enrichment). Aptamers are able to bind an ample range of non-nucleic acid targets with great specificity and affinity. Devices based on aptamers as bio-recognition elements open up a new generation of biosensors called aptasensors. This review focuses on some recent achievements in the design of advanced label-free optical aptasensors using porous silicon (PSi) as a transducer surface for the detection of pathogenic microorganisms and diagnostic molecules with high sensitivity, reliability and low limit of detection (LoD).

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