Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging

2010 ◽  
Author(s):  
Pierre Bon ◽  
Benoit Wattellier ◽  
Serge Monneret ◽  
Didier Marguet
Keyword(s):  
Shearing Interferometry ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Long Duration ◽  
Lateral Shearing ◽  
Lateral Shearing Interferometry

scholarly journals Quantitative Phase Imaging Biological Applications using Quadriwave Lateral Shearing Interferometry

2018 ◽  
Vol 114 (3) ◽  
pp. 347a
Author(s):  
Sherazade Aknoun ◽  
Antoine Federici ◽  
Flor A. Medina ◽  
Pierre Bon ◽  
Julien Savatier ◽  
...  
Keyword(s):  
Phase Imaging ◽  
Biological Applications ◽  
Quantitative Phase Imaging ◽  
Shearing Interferometry ◽  
Quantitative Phase ◽  
Lateral Shearing ◽  
Lateral Shearing Interferometry

scholarly journals Characterization of Liposomes Using Quantitative Phase Microscopy (QPM)

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Jennifer Cauzzo ◽  
Nikhil Jayakumar ◽  
Balpreet Singh Ahluwalia ◽  
Azeem Ahmad ◽  
Nataša Škalko-Basnet
Keyword(s):  
Rapid Development ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Batch Mode ◽  
Full Field ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Tracking Ability

The rapid development of nanomedicine and drug delivery systems calls for new and effective characterization techniques that can accurately characterize both the properties and the behavior of nanosystems. Standard methods such as dynamic light scattering (DLS) and fluorescent-based assays present challenges in terms of system’s instability, machine sensitivity, and loss of tracking ability, among others. In this study, we explore some of the downsides of batch-mode analyses and fluorescent labeling, while introducing quantitative phase microscopy (QPM) as a label-free complimentary characterization technique. Liposomes were used as a model nanocarrier for their therapeutic relevance and structural versatility. A successful immobilization of liposomes in a non-dried setup allowed for static imaging conditions in an off-axis phase microscope. Image reconstruction was then performed with a phase-shifting algorithm providing high spatial resolution. Our results show the potential of QPM to localize subdiffraction-limited liposomes, estimate their size, and track their integrity over time. Moreover, QPM full-field-of-view images enable the estimation of a single-particle-based size distribution, providing an alternative to the batch mode approach. QPM thus overcomes some of the drawbacks of the conventional methods, serving as a relevant complimentary technique in the characterization of nanosystems.

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