In vivo deep tissue imaging with long wavelength multiphoton excitation

2010 ◽  
Author(s):  
Demirhan Kobat ◽  
Michael E. Durst ◽  
Nozomi Nishimura ◽  
Angela W. Wong ◽  
Chris B. Schaffer ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Multiphoton Excitation ◽  
Deep Tissue ◽  
Long Wavelength ◽  
Deep Tissue Imaging

The one-pot synthesis of core/shell/shell CdTe/CdSe/ZnSe quantum dots in aqueous media for in vivo deep tissue imaging

2011 ◽  
Vol 21 (9) ◽  
pp. 2877 ◽  
Author(s):  
Shohei Taniguchi ◽  
Mark Green ◽  
Sarwat B. Rizvi ◽  
Alexander Seifalian
Keyword(s):  
Quantum Dots ◽  
Aqueous Media ◽  
Tissue Imaging ◽  
Core Shell ◽  
Deep Tissue ◽  
One Pot ◽  
One Pot Synthesis ◽  
Deep Tissue Imaging ◽  
The One

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

scholarly journals Label-free microendoscopy using a micro-needle imaging probe for in vivo deep tissue imaging

2020 ◽  
Vol 11 (9) ◽  
pp. 4976
Author(s):  
Kwanjun Park ◽  
June Hoan Kim ◽  
Taedong Kong ◽  
Woong Sun ◽  
Jonghwan Lee ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Label Free ◽  
Deep Tissue ◽  
Imaging Probe ◽  
Deep Tissue Imaging

Five-nanometer ZnSn2O4:Cr,Eu ultra-small nanoparticles as new near infrared-emitting persistent luminescent nanoprobes for cellular and deep tissue imaging at 800 nm

Nanoscale ◽  
2017 ◽  
Vol 9 (25) ◽  
pp. 8631-8638 ◽  
Author(s):  
Jin-Lei Li ◽  
Jun-Peng Shi ◽  
Cheng-Cheng Wang ◽  
Peng-Hui Li ◽  
Zhen-Feng Yu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Small Nanoparticles

Schematic illustration of the synthesis, functionalization and repeated in vivo simulated deep tissue imaging of ZSO NPLNPs.

3D printed optical phantoms and deep tissue imaging for in vivo applications including oral surgery

2017 ◽  
Author(s):  
Brian Z. Bentz ◽  
Alfonso Costas ◽  
Vaibhav Gaind ◽  
Jose M. Garcia ◽  
Kevin J. Webb
Keyword(s):  
Oral Surgery ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
3D Printed

Ablation of Red Stable Transfected Prostate Cancer Cell Lines by C-CPE Gold-Nanoparticle Mediated Laser Intervention

2021 ◽  
Author(s):  
Suhayla Alnajjar ◽  
Ingo Nolte ◽  
Annegret Becker ◽  
Tina Kostka ◽  
Jan Torben Schille ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticle ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging

Abstract Background: Claudin (CLDN) proteins have been described to be found and accordingly targeted to evaluate novel therapeutic approaches. C-terminus of Clostridium perfringens enterotoxin (C-CPE) binds efficiently several claudins and thus recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for cancer cell targeting using gold nanoparticle- mediated laser perforation (GNOME-LP). Cancer cells inoculation is routinely used to generate in vivo models to evaluate novel therapeutic approaches in prostate cancer. However, detailed characterization of cancer spreading and early tumor development and therapeutic response is often limited as conventional cell lines do not allow advanced deep tissue imaging.Methods: two canine prostate cancer cell lines were stably transfected with red fluorescent protein (RFP), followed by G418 selection. RFP marker as well as CLDN3, -4 and -7 expression was comparatively confirmed by flow cytometry, qPCR and immunofluorescences. For cancer cells targeting, GNOME-LP at a laser fluence of 72 mJ/cm² and a scanning speed of 0.5 cm/s was used. Statistical analysis was performed using SAS software 7.1, Dunnett´s Multiple Comparison Test and Student´s two-sided t-test. Differences were considered statistically significant for p<0.05.Results: we established two canine prostate carcinoma cell lines, stably expressing RFP allowing perspective deep tissue imaging. Directed C-CPE-AuNP binding to native and RFP transfected cells verified the capability to specifically target CLDN receptors. Cancer cell ablation was demonstrated in vitro setting using a combination of gold nanoparticle mediated laser perforation and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10 % depending on cell line. Conclusion: the results confirm that this therapeutic approach can be used efficiently to target prostate carcinoma cells carrying a marker protein allowing deep tissue imaging. The established cell lines and the verified proof of concept in vitro study provide the basis for perspective Xenograft model in vivo studies. The introduce red fluorescence enables deep tissue imaging in living animals and therefore detailed characterization of tumor growth and subsequently possible tumor ablation through C-CPE-AuNPs treatment.

scholarly journals A far-red fluorescent chemogenetic reporter for in vivo molecular imaging

2020 ◽  
Author(s):  
Chenge Li ◽  
Alison G. Tebo ◽  
Marion Thauvin ◽  
Marie-Aude Plamont ◽  
Michel Volovitch ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Zebrafish Embryo ◽  
Tissue Imaging ◽  
Live Cells ◽  
Protein Protein Interactions ◽  
Deep Tissue ◽  
Red Fluorescence ◽  
Genetic Encoding ◽  
Deep Tissue Imaging

AbstractFar-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae and chicken embryo. Beyond enabling genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.

scholarly journals Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy

2011 ◽  
Vol 17 (4) ◽  
pp. 614-617 ◽  
Author(s):  
Sherry G. Clendenon ◽  
Pamela A. Young ◽  
Michael Ferkowicz ◽  
Carrie Phillips ◽  
Kenneth W. Dunn
Keyword(s):  
Image Quality ◽  
Confocal Microscopy ◽  
Multiphoton Microscopy ◽  
Fluorescent Imaging ◽  
Tissue Imaging ◽  
Multiphoton Excitation ◽  
Deep Tissue ◽  
Tissue Clearing ◽  
Deep Tissue Imaging ◽  
Imaging Depth

AbstractIn scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.

scholarly journals Multiplexed aberration measurement for deep tissue imaging in vivo

2014 ◽  
Vol 11 (10) ◽  
pp. 1037-1040 ◽  
Author(s):  
Chen Wang ◽  
Rui Liu ◽  
Daniel E Milkie ◽  
Wenzhi Sun ◽  
Zhongchao Tan ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging
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