Detection system of capillary array electrophoresis microchip based on optical fiber

2009 ◽  
Author(s):  
Xiaobo Yang ◽  
Haiming Bai ◽  
Weiping Yan
Keyword(s):  
Optical Fiber ◽  
Detection System ◽  
Capillary Array Electrophoresis ◽  
Capillary Array ◽  
Electrophoresis Microchip

Study of Stray Light and Cross-Talk of the Capillary Array Electrophoresis

2007 ◽  
Vol 364-366 ◽  
pp. 1128-1132
Author(s):  
Jie Wang ◽  
Li Qiang Wang ◽  
Yan Shi ◽  
Hua Zheng ◽  
Zu Kang Lu
Keyword(s):  
Fluorescence Detection ◽  
Cross Talk ◽  
Laser Scanning ◽  
Detection System ◽  
Laser Induced Fluorescence ◽  
Stray Light ◽  
Laser Induced Fluorescence Detection ◽  
Capillary Array Electrophoresis ◽  
Inner Diameter ◽  
Capillary Array

Capillary array electrophoresis (CAE) is founded with the laser induced fluorescence detection (LIFD) system. The same model as the detection system is simulated in Tracepro and the stray light caused by capillaries is analyzed. The stray light distribution of the observation surface is plotted when the laser scans the different sections of a capillary in the array. The results of simulation indicate that the stray light is strongest when scanning the inner center of a capillary and decreases at edges; in two cases (50μm and 75μm inner diameter, ID) when the ID is larger, the stray light is stronger as a whole. Different ID of capillaries which affects the stray light is analyzed; considering many factors, 50μm ID of capillary is appropriate. Based on the analysis of stray light cross-talk by other capillaries in an array, the spacing between capillaries in an array is suggested more than 50μm which the stray light is steady-going and simple to operate. The results can be helpful for the mode of the laser scanning different capillaries in an array sequentially.

MICRO CAPILLARY ARRAY ELECTROPHORESIS CHIP BY MOVING MASK LIGA TECHNOLOGY

2004 ◽  
Vol 01 (01) ◽  
pp. 39-46
Author(s):  
HUI YOU ◽  
SHOUJI SHINOHARA ◽  
KENNICHI ENAMI ◽  
SHINSUKE SHIBATA ◽  
OSAMU TABATA ◽  
...  
Keyword(s):  
Adhesive Bonding ◽  
Dna Analysis ◽  
Adhesive Layer ◽  
Fabrication Process ◽  
Optical Components ◽  
X Ray ◽  
Laser Induced Fluorescence Detection ◽  
Capillary Array Electrophoresis ◽  
Liga Technology ◽  
Capillary Array

The concept and the fabrication process of a micro capillary array electrophoresis (μ-CAE) for DNA analysis were stated. The chip was mainly made of plastic and fabricated by the innovatory LIGA technology which included moving mask deep X-ray lithography, electroplating and hot embossing, and assembled by an adhesive bonding, in which the adhesive layer solidified and formed firm bond under UV exposure. The micro channel array with high aspect ratio is the key part, whose wall should have a slight inclination to ensure the demoulding. It was demonstrated that moving mask deep X-ray lithography (M2DXL) technology could successfully control the inclination and enabled to integrate micro optical components such as micro lens into the chip, which improved detection performance greatly. The fabrication process was experimented and some initial prototype chips have been obtained. Fluidic test and electrophoresis test with laser-induced fluorescence detection has been done on the prototype. The results confirm that the μ-CAE by moving mask LIGA process is realizable and it has great potential in high-throughput, which may lead to ultra-fast DNA analysis.

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