Intravital fiber-optic fluorescence imaging for monitoring ovarian carcinoma progression and treatment response

2009 ◽  
Author(s):  
Bryan Q. Spring ◽  
Jonathan P. Celli ◽  
Conor L. Evans ◽  
Wei Zhong ◽  
Imran Rizvi ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Treatment Response ◽  
Fluorescence Imaging ◽  
Fiber Optic

scholarly journals Non-invasive monitoring of arthritis treatment response via targeting of tyrosine-phosphorylated annexin A2 in chondrocytes

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Shaw-Wei D. Tsen ◽  
Luke E. Springer ◽  
Krishna Sharmah Gautam ◽  
Rui Tang ◽  
Kexian Liang ◽  
...  
Keyword(s):  
Drug Treatment ◽  
Treatment Response ◽  
Fluorescence Imaging ◽  
Mouse Models ◽  
Near Infrared ◽  
Early Response ◽  
Annexin A2 ◽  
Whole Body ◽  
Invasive Monitoring ◽  
Non Invasive

Abstract Background The development and optimization of therapies for rheumatoid arthritis (RA) is currently hindered by a lack of methods for early non-invasive monitoring of treatment response. Annexin A2, an inflammation-associated protein whose presence and phosphorylation levels are upregulated in RA, represents a potential molecular target for tracking RA treatment response. Methods LS301, a near-infrared dye-peptide conjugate that selectively targets tyrosine 23-phosphorylated annexin A2 (pANXA2), was evaluated for its utility in monitoring disease progression, remission, and early response to drug treatment in mouse models of RA by fluorescence imaging. The intraarticular distribution and localization of LS301 relative to pANXA2 was determined by histological and immunohistochemical methods. Results In mouse models of spontaneous and serum transfer-induced inflammatory arthritis, intravenously administered LS301 showed selective accumulation in regions of joint pathology including paws, ankles, and knees with positive correlation between fluorescent signal and disease severity by clinical scoring. Whole-body near-infrared imaging with LS301 allowed tracking of spontaneous disease remission and the therapeutic response after dexamethasone treatment. Histological analysis showed preferential accumulation of LS301 within the chondrocytes and articular cartilage in arthritic mice, and colocalization was observed between LS301 and pANXA2 in the joint tissue. Conclusions We demonstrate that fluorescence imaging with LS301 can be used to monitor the progression, remission, and early response to drug treatment in mouse models of RA. Given the ease of detecting LS301 with portable optical imaging devices, the agent may become a useful early treatment response reporter for arthritis diagnosis and drug evaluation.

Fiber-optic laser scanning confocal microscope suitable for fluorescence imaging

1994 ◽  
Vol 33 (4) ◽  
pp. 573 ◽  
Author(s):  
Peter M. Delaney ◽  
Martin R. Harris ◽  
Roger G. King
Keyword(s):  
Fluorescence Imaging ◽  
Laser Scanning ◽  
Fiber Optic ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

High resolution fluorescence imaging with cantilevered near‐field fiber optic probes

1996 ◽  
Vol 69 (25) ◽  
pp. 3809-3811 ◽  
Author(s):  
Chad E. Talley ◽  
Gregory A. Cooksey ◽  
Robert C. Dunn
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Fiber Optic ◽  
Near Field ◽  
Fiber Optic Probes

scholarly journals CD24-targeted fluorescence imaging in patient-derived xenograft models of high-grade serous ovarian carcinoma

EBioMedicine ◽  
2020 ◽  
Vol 56 ◽  
pp. 102782 ◽  
Author(s):  
Katrin Kleinmanns ◽  
Katharina Bischof ◽  
Shamundeeswari Anandan ◽  
Mihaela Popa ◽  
Lars A. Akslen ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Fluorescence Imaging ◽  
High Grade ◽  
Patient Derived Xenograft ◽  
Serous Ovarian Carcinoma ◽  
Xenograft Models

scholarly journals IPI59: An Actionable Biomarker to Improve Treatment Response in Serous Ovarian Carcinoma Patients

2017 ◽  
Vol 9 (1) ◽  
pp. 1-12
Author(s):  
J. Choi ◽  
S. Ye ◽  
K. H. Eng ◽  
K. Korthauer ◽  
W. H. Bradley ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Treatment Response ◽  
Improve Treatment ◽  
Serous Ovarian Carcinoma

scholarly journals Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo

2008 ◽  
Vol 16 (8) ◽  
pp. 5556 ◽  
Author(s):  
Christoph J. Engelbrecht ◽  
Richard S. Johnston ◽  
Eric J. Seibel ◽  
Fritjof Helmchen
Keyword(s):  
Fluorescence Imaging ◽  
Fiber Optic ◽  
Two Photon

scholarly journals An all-fiber-optic endoscopy platform for simultaneous OCT and fluorescence imaging

2012 ◽  
Vol 3 (11) ◽  
pp. 2851 ◽  
Author(s):  
Jessica Mavadia ◽  
Jiefeng Xi ◽  
Yongping Chen ◽  
Xingde Li
Keyword(s):  
Fluorescence Imaging ◽  
Fiber Optic ◽  
Optic Endoscopy

scholarly journals Fluorescence-guided stereotactic biopsy: a proof-of-concept study

2020 ◽  
Vol 132 (2) ◽  
pp. 530-536
Author(s):  
Robert Lynagh ◽  
Mark Ishak ◽  
Joseph Georges ◽  
Danielle Lopez ◽  
Hany Osman ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Fluorescence Intensity ◽  
Stereotactic Biopsy ◽  
Fiber Optic ◽  
Normal Brain ◽  
Blue Fluorescence ◽  
Tumor Implantation

OBJECTIVEAccurate histopathological diagnoses are often necessary for treating neuro-oncology patients. However, stereotactic biopsy (STB), a common method for obtaining suspicious tissue from deep or eloquent brain regions, fails to yield diagnostic tissue in some cases. Failure to obtain diagnostic tissue can delay initiation of treatment and may result in further invasive procedures for patients. In this study, the authors sought to determine if the coupling of in vivo optical imaging with an STB system is an effective method for identification of diagnostic tissue at the time of biopsy.METHODSA minimally invasive fiber optic imaging system was developed by coupling a 0.65-mm-diameter coherent fiber optic fluorescence microendoscope to an STB system. Human U251 glioma cells were transduced for stable expression of blue fluorescent protein (BFP) to produce U251-BFP cells that were utilized for in vitro and in vivo experiments. In vitro, blue fluorescence was confirmed, and tumor cell delineation by fluorescein sodium (FNa) was quantified with fluorescence microscopy. In vivo, transgenic athymic rats implanted with U251-BFP cells (n = 4) were utilized for experiments. Five weeks postimplantation, the rats received 5–10 mg/kg intravenous FNa and underwent craniotomies overlying the tumor implantation site and contralateral normal brain. A clinical STB needle containing our 0.65-mm imaging fiber was passed through each craniotomy and images were collected. Fluorescence images from regions of interest ipsilateral and contralateral to tumor implantation were obtained and quantified.RESULTSLive-cell fluorescence imaging confirmed blue fluorescence from transduced tumor cells and revealed a strong correlation between tumor cells quantified by blue fluorescence and FNa contrast (R2 = 0.91, p < 0.001). Normalized to background, in vivo FNa-mediated fluorescence intensity was significantly greater from tumor regions, verified by blue fluorescence, compared to contralateral brain in all animals (301.7 ± 34.18 relative fluorescence units, p < 0.001). Fluorescence intensity measured from the tumor margin was not significantly greater than that from normal brain (p = 0.89). Biopsies obtained from regions of strong fluorescein contrast were histologically consistent with tumor.CONCLUSIONSThe authors found that in vivo fluorescence imaging with an STB needle containing a submillimeter-diameter fiber optic fluorescence microendoscope provided direct visualization of neoplastic tissue in an animal brain tumor model prior to biopsy. These results were confirmed in vivo with positive control cells and by post hoc histological assessment. In vivo fluorescence guidance may improve the diagnostic yield of stereotactic biopsies.

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