Real time micro/nano particle detection and tracking with nanosecond resolution

2009 ◽  
Author(s):  
Feng Qian ◽  
Qi Song ◽  
En-kuang Tien ◽  
Ozdal Boyraz
Keyword(s):  
Real Time ◽  
Nano Particle ◽  
Particle Detection ◽  
Detection And Tracking

scholarly journals Deciphering dynamics of clathrin-mediated endocytosis in a living organism

2016 ◽  
Vol 214 (3) ◽  
pp. 347-358 ◽  
Author(s):  
Joshua P. Ferguson ◽  
Nathan M. Willy ◽  
Spencer P. Heidotting ◽  
Scott D. Huber ◽  
Matthew J. Webber ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Real Time ◽  
Growth Rates ◽  
Cultured Cells ◽  
Living Organism ◽  
Particle Detection ◽  
Detection And Tracking ◽  
Spatiotemporal Changes ◽  
Multicellular Organisms

Current understanding of clathrin-mediated endocytosis (CME) dynamics is based on detection and tracking of fluorescently tagged clathrin coat components within cultured cells. Because of technical limitations inherent to detection and tracking of single fluorescent particles, CME dynamics is not characterized in vivo, so the effects of mechanical cues generated during development of multicellular organisms on formation and dissolution of clathrin-coated structures (CCSs) have not been directly observed. Here, we use growth rates of fluorescence signals obtained from short CCS intensity trace fragments to assess CME dynamics. This methodology does not rely on determining the complete lifespan of individual endocytic assemblies. Therefore, it allows for real-time monitoring of spatiotemporal changes in CME dynamics and is less prone to errors associated with particle detection and tracking. We validate the applicability of this approach to in vivo systems by demonstrating the reduction of CME dynamics during dorsal closure of Drosophila melanogaster embryos.

Real time detection and tracking of Gauzes by RFID UWB technique

2010 ◽  
Author(s):  
S. Boncinelli ◽  
P. Citti ◽  
E. Del Re ◽  
G. Campatelli ◽  
L. Pierucci ◽  
...  
Keyword(s):  
Real Time ◽  
Detection And Tracking ◽  
Real Time Detection
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