Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements

2009 ◽  
Author(s):  
Steffen Hackbarth ◽  
Jan Schlothauer ◽  
Annegret Preuß ◽  
Beate Röder
Keyword(s):  
Singlet Oxygen ◽  
Spatial Resolution ◽  
Living Cells ◽  
Time Resolved ◽  
Oxygen Kinetics ◽  
Time Resolved Measurements

Microscopic time-resolved imaging of singlet oxygen by delayed fluorescence in living cells

2017 ◽  
Vol 16 (11) ◽  
pp. 1643-1653 ◽  
Author(s):  
Marek Scholz ◽  
Roman Dědic ◽  
Jan Hála
Keyword(s):  
Singlet Oxygen ◽  
Living Cells ◽  
Delayed Fluorescence ◽  
Time Resolved ◽  
Microscopic Imaging ◽  
Time Resolved Imaging ◽  
In Cells

Singlet Oxygen Feedback Delayed Fluorescence (SOFDF) is a novel semi-direct modality for microscopic imaging of singlet oxygen in cells. SOFDF enables time-resolved experiments and it can be much stronger than the singlet oxygen phosphorescence.

New insights to primary photodynamic effects – Singlet oxygen kinetics in living cells

2010 ◽  
Vol 98 (3) ◽  
pp. 173-179 ◽  
Author(s):  
Steffen Hackbarth ◽  
Jan Schlothauer ◽  
Annegret Preuß ◽  
Beate Röder
Keyword(s):  
Singlet Oxygen ◽  
Living Cells ◽  
Oxygen Kinetics

Time-Resolved Luminescent High-Throughput Screening Platform for Lysosomotropic Compounds in Living Cells

ACS Sensors ◽  
2020 ◽  
Author(s):  
Ke-Jia Wu ◽  
Chun Wu ◽  
Feng Chen ◽  
Sha-Sha Cheng ◽  
Dik-Lung Ma ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Living Cells ◽  
Time Resolved ◽  
Screening Platform

scholarly journals Photophysical Properties of Protoporphyrin IX, Pyropheophorbide-a, and Photofrin® in Different Conditions

2021 ◽  
Vol 14 (2) ◽  
pp. 138
Author(s):  
Bauyrzhan Myrzakhmetov ◽  
Philippe Arnoux ◽  
Serge Mordon ◽  
Samir Acherar ◽  
Irina Tsoy ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Photophysical Properties ◽  
Protoporphyrin Ix ◽  
Fluorescence Emission ◽  
Solvent Polarity ◽  
Visible Absorption ◽  
Light Dosimetry ◽  
Time Resolved ◽  
Pyropheophorbide A ◽  
Influence Of Solvent

Photodynamic therapy (PDT) is an innovative treatment of malignant or diseased tissues. The effectiveness of PDT depends on light dosimetry, oxygen availability, and properties of the photosensitizer (PS). Depending on the medium, photophysical properties of the PS can change leading to increase or decrease in fluorescence emission and formation of reactive oxygen species (ROS) especially singlet oxygen (1O2). In this study, the influence of solvent polarity, viscosity, concentration, temperature, and pH medium on the photophysical properties of protoporphyrin IX, pyropheophorbide-a, and Photofrin® were investigated by UV-visible absorption, fluorescence emission, singlet oxygen emission, and time-resolved fluorescence spectroscopies.

scholarly journals Super-resolution time-resolved imaging using computational sensor fusion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
C. Callenberg ◽  
A. Lyons ◽  
D. den Brok ◽  
A. Fatima ◽  
A. Turpin ◽  
...  
Keyword(s):  
Sensor Fusion ◽  
Spatial Resolution ◽  
Temporal Resolution ◽  
Single Photon ◽  
Super Resolution ◽  
Numerical Data ◽  
Data Cube ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Temporal Precision

AbstractImaging across both the full transverse spatial and temporal dimensions of a scene with high precision in all three coordinates is key to applications ranging from LIDAR to fluorescence lifetime imaging. However, compromises that sacrifice, for example, spatial resolution at the expense of temporal resolution are often required, in particular when the full 3-dimensional data cube is required in short acquisition times. We introduce a sensor fusion approach that combines data having low-spatial resolution but high temporal precision gathered with a single-photon-avalanche-diode (SPAD) array with data that has high spatial but no temporal resolution, such as that acquired with a standard CMOS camera. Our method, based on blurring the image on the SPAD array and computational sensor fusion, reconstructs time-resolved images at significantly higher spatial resolution than the SPAD input, upsampling numerical data by a factor $$12 \times 12$$ 12 × 12 , and demonstrating up to $$4 \times 4$$ 4 × 4 upsampling of experimental data. We demonstrate the technique for both LIDAR applications and FLIM of fluorescent cancer cells. This technique paves the way to high spatial resolution SPAD imaging or, equivalently, FLIM imaging with conventional microscopes at frame rates accelerated by more than an order of magnitude.

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