Ultra-high sensitivity nanoplasmonic resonance energy transfer spectroscopic biomolecular imaging

2009 ◽  
Author(s):  
G. Logan Liu
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Biomolecular Imaging

Synthesis and Detection Experiments of a Biomolecule Detection Probe Based on Fluorescence Changes

2014 ◽  
Vol 998-999 ◽  
pp. 336-339
Author(s):  
Jun Wang ◽  
Da Hai Ren
Keyword(s):  
Energy Transfer ◽  
Fluorescence Intensity ◽  
Fluorescence Probe ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Fluorescence Probes ◽  
Biomolecule Detection ◽  
Detection Probe ◽  
Higher Sensitivity

The sensitivity of fluorescence probes built upon the resonance energy transfer is not high enough at present. We built a fluorescence probe with high sensitivity (SA-488-sub-nanogold) by means of the fluorochrome Alexa488 (SA-488) labeled by streptavidin, nanogold, and biotin-subpeptide. When the fluorescence molecule SA-488 binds with the nanogold by biotin-subpeptide, the fluorescence intensity will be suppressed because of resonance energy transfer. If there are molecules under test, the energy transfer will be blocked, by which we can get the molecule content from the fluorescence intensity. Using this probe, we acquired a lower detection limit and a higher sensitivity for biotin detection.

A boron difluoride dye showing the aggregation-induced emission feature and high sensitivity to intra- and extra-cellular pH changes

2016 ◽  
Vol 52 (3) ◽  
pp. 541-544 ◽  
Author(s):  
Dan Wu ◽  
Li Shao ◽  
Yang Li ◽  
Qinglian Hu ◽  
Feihe Huang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescent Probe ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Emission Feature ◽  
Förster Resonance Energy Transfer ◽  
Aggregation Induced Emission ◽  
Ph Changes ◽  
Boron Difluoride

A novel AIE-active boron difluoride fluorescent probe P3T was designed and synthesized. P3T exhibited high sensitivity to intra- and extra-cellular pH changes. Furthermore, a Förster resonance energy transfer (FRET) system was constructed.

Luminescence Energy Transfer–Based Screening and Target Engagement Approaches for Chemical Biology and Drug Discovery

2021 ◽  
pp. 247255522110360
Author(s):  
Eun Jeong Cho ◽  
Kevin N. Dalby
Keyword(s):  
Energy Transfer ◽  
Drug Discovery ◽  
Early Stage ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Live Cells ◽  
Molecular Binding ◽  
Compound Screening ◽  
Homogeneous Assay

Luminescence is characterized by the spontaneous emission of light resulting from either chemical or biological reactions. Because of their high sensitivity, reduced background interference, and applicability to numerous situations, luminescence-based assay strategies play an essential role in early-stage drug discovery. Newer developments in luminescence-based technologies have dramatically affected the ability of researchers to investigate molecular binding events. At the forefront of these developments are the nano bioluminescence resonance energy transfer (NanoBRET) and amplified luminescent proximity homogeneous assay (Alpha) technologies. These technologies have opened up numerous possibilities for analyzing the molecular biophysical properties of complexes in environments such as cell lysates. Moreover, NanoBRET enables the validation and quantitation of the interactions between therapeutic targets and small molecules in live cells, representing an essential benchmark for preclinical drug discovery. Both techniques involve proximity-based luminescence energy transfer, in which excited-state energy is transferred from a donor to an acceptor, where the efficiency of transfer depends on proximity. Both approaches can be applied to high-throughput compound screening in biological samples, with the NanoBRET assay providing opportunities for live-cell screening. Representative applications of both technologies for assessing physical interactions and associated challenges are discussed.

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