The determination of epigenetic modification, especially that of 5-methylcytosine in the CpG sequence in mammals, has attracted attention because it should prove valuable in a wide range of research fields including diagnosis, drug discovery and therapy. In this review, we introduce the recent development of bisulfite-free DNA methylation analysis, which we classify into two categories, namely labelling-based and labelling-free assays.
DNA methylation analysis of selected genes for the detection
of early-stage lung cancer using circulating cell-free DNA