There are many approaches to obtaining high-resolution images and three dimensional volumetric data sets, but all have limitations. Many techniques involve reconstructing volumes of information from sections, either physical sections or optical sections. Recently, James Sharpe, Ulf Ahlgren, Paul Perry, Bill Hill, Allyson Ross, Jacob Hecksher-Sørensen, Richard Baldock, and Duncan Davidson have developed an optical technique that is analogous to computed tomography (CT). Whereas clinical CT involves an X-ray source and detector rotating around the patient, optical projection tomography (OPT) has the specimen rotating within an optical pathway.
In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography