Digital image method in quantitative analysis usually uses one of the RGB primary color components (Red, Green, Blue), so that not all digital image data can be extracted. Then needed a method that can render the whole RGB values as variables in quantitative analysis are known as chemometric. This research aims to know the influence of the application of chemometric against the sensitivity of the digital image. Chemometry method used is the Principal Component Regression (PCR) and Partial Least Square (PLS) using Unscramber X software from Camo software, USA.. This method is applied for the quantitative analysis of Mercury (II) ion with silver nanoparticles (NPP) immobilization on filter paper indicator. The research results showed that chemometric has a good influence against the level of the Limit of Detection (LOD) of the digital image, where the level of LOD with chemometric application of the Principal Component Regression (PCR) is 0.4311 ppb, and Partial Least Square (PLS) is 0.4310 ppb smaller than without the application of chemometric Single Linear Regression (SLR) at 0.837 ppb.
Quantitative analysis ofex vivocolorectal epithelium using an automated feature extraction algorithm for microendoscopy image data