Stimulated Raman scattering microscopy for biomedical imaging

2009 ◽  
Author(s):  
Wei Min ◽  
Christian W. Freudiger ◽  
Sijia Lu ◽  
Chengwei He ◽  
Jing X. Kang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Biomedical Imaging ◽  
Stimulated Raman Scattering ◽  
Stimulated Raman

scholarly journals Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

Science ◽  
2008 ◽  
Vol 322 (5909) ◽  
pp. 1857-1861 ◽  
Author(s):  
Christian W. Freudiger ◽  
Wei Min ◽  
Brian G. Saar ◽  
Sijia Lu ◽  
Gary R. Holtom ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Biomedical Imaging ◽  
Stimulated Raman Scattering ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Raman Microscopy ◽  
Label Free ◽  
Omega 3 ◽  
Low Sensitivity ◽  
Stimulated Raman

Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis.

scholarly journals Label-Free Biomedical Imaging Using High-Speed Lock-In Pixel Sensor for Stimulated Raman Scattering

Sensors ◽  
2017 ◽  
Vol 17 (11) ◽  
pp. 2581 ◽  
Author(s):  
Kamel Mars ◽  
De Xing Lioe ◽  
Shoji Kawahito ◽  
Keita Yasutomi ◽  
Keiichiro Kagawa ◽  
...  
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Biomedical Imaging ◽  
Stimulated Raman Scattering ◽  
Label Free ◽  
Lock In ◽  
Stimulated Raman

Ultra-violet Stimulated Raman Scattering of SrWO4 Crystal

2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Ultra Violet ◽  
Stimulated Raman

Bulk Laser Damage Threshold and Stimulated Raman Scattering in KTiOPO4 Crystal.

1996 ◽  
Vol 24 (8) ◽  
pp. 906-909
Author(s):  
Akio MIYAMOTO ◽  
Hidetsugu YOSHIDA ◽  
Yusuke MORI ◽  
Takatomo SASAKI ◽  
Sadao NAKAI
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Damage Threshold ◽  
Laser Damage Threshold ◽  
Laser Damage ◽  
Stimulated Raman
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