Dual window method for processing spectroscopic optical coherence tomography signals with high spectral and spatial resolution

2009 ◽  
Author(s):  
Francisco E. Robles ◽  
Robert N. Graf ◽  
Adam Wax
Keyword(s):  
Optical Coherence Tomography ◽  
Spatial Resolution ◽  
Optical Coherence ◽  
Window Method ◽  
Dual Window

scholarly journals Real-time speckle reduction in optical coherence tomography using the dual window method

2018 ◽  
Vol 9 (2) ◽  
pp. 616 ◽  
Author(s):  
Yang Zhao ◽  
Kengyeh K. Chu ◽  
Will J. Eldridge ◽  
Evan T. Jelly ◽  
Michael Crose ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Speckle Reduction ◽  
Optical Coherence ◽  
Window Method ◽  
Dual Window

scholarly journals En Face and Cross-sectional Corneal Tomograms Using Sub-micron spatial resolution Optical Coherence Tomography

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Yu-Tung Chen ◽  
Chia-Ying Tsai ◽  
Yu-Kuang Chiu ◽  
Ting-Wei Hsu ◽  
Lily Wei Chen ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Spatial Resolution ◽  
Optical Coherence ◽  
Cross Sectional ◽  
En Face

scholarly journals Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chia-Ying Tsai ◽  
Cheng-Hung Shih ◽  
Hsiao-Sang Chu ◽  
Yi-Ting Hsieh ◽  
Sheng-Lung Huang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Cell Therapy ◽  
Cell Line ◽  
Spatial Resolution ◽  
Cell Sheet ◽  
Live Cells ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Medicine Research

AbstractThree-dimensional (3D) configuration of in vitro cultivated cells has been recognised as a valuable tool in developing stem cell and cancer cell therapy. However, currently available imaging approaches for live cells have drawbacks, including unsatisfactory resolution, lack of cross-sectional and 3D images, and poor penetration of multi-layered cell products, especially when cells are cultivated on semitransparent carriers. Herein, we report a prototype of a full-field optical coherence tomography (FF-OCT) system with isotropic submicron spatial resolution in en face and cross-sectional views that provides a label-free, non-invasive platform with high-resolution 3D imaging. We validated the imaging power of this prototype by examining (1) cultivated neuron cells (N2A cell line); (2) multilayered, cultivated limbal epithelial sheets (mCLESs); (3) neuron cells (N2A cell line) and mCLESs cultivated on a semitransparent amniotic membrane (stAM); and (4) directly adherent colonies of neuron-like cells (DACNs) covered by limbal epithelial cell sheets. Our FF-OCT exhibited a penetrance of up to 150 μm in a multilayered cell sheet and displayed the morphological differences of neurons and epithelial cells in complex coculture systems. This FF-OCT is expected to facilitate the visualisation of cultivated cell products in vitro and has a high potential for cell therapy and translational medicine research.

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