Increased in vivo skin penetration of quantum dots with UVR and in vitro quantum dot cytotoxicity

2009 ◽  
Author(s):  
Luke Mortensen ◽  
Hong Zheng ◽  
Renea Faulknor ◽  
Anna De Benedetto ◽  
Lisa Beck ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Skin Penetration ◽  
In Vivo Skin Penetration

scholarly journals In Vivo Skin Penetration of Quantum Dot Nanoparticles in the Murine Model: The Effect of UVR

Nano Letters ◽  
2008 ◽  
Vol 8 (9) ◽  
pp. 2779-2787 ◽  
Author(s):  
Luke J. Mortensen ◽  
Gunter Oberdörster ◽  
Alice P. Pentland ◽  
Lisa A. DeLouise
Keyword(s):  
Quantum Dot ◽  
Murine Model ◽  
Skin Penetration ◽  
In Vivo Skin Penetration

Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging

2017 ◽  
Vol 53 (68) ◽  
pp. 9450-9453 ◽  
Author(s):  
Setsuko Tsuboi ◽  
Akira Sasaki ◽  
Takao Sakata ◽  
Hidehiro Yasuda ◽  
Takashi Jin
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Breast Tumor ◽  
Protein G ◽  
Breast Tumor Cells ◽  
B1 Domain ◽  
Antibody Conjugation ◽  
Immunoglobulin Binding

A facile method for the preparation of antibody–quantum dot conjugates using the immunoglobulin binding (B1) domain of protein G is presented. The utility of antibody–quantum dot conjugates using the B1 domain is demonstrated for fluorescence imaging of breast tumor cellsin vitroandin vivo.

381 LABELING AND ANALYSIS OF SWINE ADIPOSE-DERIVED STEM CELLS WITH CARBOXYFLUORESCEIN DIACETATE AND QUANTUM DOTS

2010 ◽  
Vol 22 (1) ◽  
pp. 347
Author(s):  
N. Cieslak ◽  
A. Massie ◽  
S. M. Wilson ◽  
E. Monaco ◽  
M. B. Wheeler
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Regenerative Medicine ◽  
Quantum Dot ◽  
In Vitro Culture ◽  
Adult Stem Cells ◽  
Attractive Alternative ◽  
Adipose Derived Stem Cells

The quantity, accessibility, and abundance of subcutaneous adipose tissue in humans make it an attractive alternative to bone marrow as a source of adult stem cells for therapeutic purposes. Adult adipose-derived mesenchymal stem cells can differentiate into a variety of lineages including adipose, bone, cartilage, and muscle. In addition, the use of adult stem cells for regenerative medicine rather than those from embryos avoids concerns with ethics, safety, and immunology. One important issue is the ability to track the transplanted stem cells during the regeneration process to evaluate the stem cell-mediated healing. The objective of this study was to compare the efficiency, longevity, and intensity of carboxyfluorescein diacetate, succinimidyl ester (CFDA SE) and quantum dot nanocrystal (Qtracker™, Invitrogen, Carlsbad, CA, USA) labeled adipose-derived stem cells (ADSC) over an in vitro culture period of 4 weeks. Adipose-derived stem cells (6 x 106) previously isolated and frozen at -196°C were thawed and cultured in 75-cm3 flasks with 14 mL of DMEM. Cells were grown to 80% confluence and trypsinized. After trypsinization, the cells were divided into 4 treatments (3 x 106 cells per treatment). The treatments were (1) unlabeled control, (2) labeled with 30 μM CFDA SE, (3) labeled with 15 nM Qtracker™, and (4) labeled with 15 nM Qtracker™, following the Invitrogen Qtracker™ protocol. Cells (1 x 106) were removed from each treatment every week for 4 weeks and fixed in formalin for later analysis. When all the samples were collected, they were analyzed using flow cytometry. Data were analyzed via chi-square test. The percentage of cells labeled with CFDA SE and Qtracker™ was 99.35 and 98.46%, respectively, immediately after labeling. By 1 wk, the percentage of cells labeled with CFDA SE and Qtracker™ had deceased (P < 0.01) to 0.11 and 1.48%, respectively. The CFDA SE-labeled cell percentages had decreased (P < 0.01) to 0% at 2, 3, and 4 wk, respectively. The Qtracker™-labeled cells also decreased (P < 0.01) to 0.745, 1.69 and 0.45% at 2, 3, and 4 wk, respectively. The high rate of cell division of these cells in vitro might be responsible for the rapid loss of both labels during the first week of culture. Previous results from our lab have shown that the CFDA SE is retained in the cells for up to 6 wk in vivo (Lima AS et al. 2006 Reprod. Fertil. Dev. 18, 208). Similar studies need to be done with the quantum dot-labeled cells to determine the Qtracker™ label’s longevity in vivo. In conclusion, quantum dots can be used to label ADSC, in vitro, for at least 4 wk, albeit at much lower levels than those observed during the week following labeling. Determination of a suitable label for high-percentage porcine ADSC labeling during long-term in vitro culture remains to be completed. This research was supported by the Intel Scholar’s Program and the Illinois Regenerative Medicine Institute.

scholarly journals In vivo skin penetration and metabolic path of quantum dots

2013 ◽  
Vol 56 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Lei Tang ◽  
ChunLing Zhang ◽  
GuangMing Song ◽  
Xun Jin ◽  
ZhongWei Xu
Keyword(s):  
Quantum Dots ◽  
Skin Penetration ◽  
In Vivo Skin Penetration

Reverse Hexagonal Phase Nanodispersion of Monoolein and Oleic Acid for Topical Delivery of Peptides: in Vitro and in Vivo Skin Penetration of Cyclosporin A

2006 ◽  
Vol 23 (6) ◽  
pp. 1332-1342 ◽  
Author(s):  
Luciana B. Lopes ◽  
Denise A. Ferreira ◽  
Daniel de Paula ◽  
M. Tereza J. Garcia ◽  
José A. Thomazini ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Cyclosporin A ◽  
Hexagonal Phase ◽  
Skin Penetration ◽  
Topical Delivery ◽  
In Vivo Skin Penetration

scholarly journals The Topical Nanodelivery of Vismodegib Enhances Its Skin Penetration and Performance In Vitro While Reducing Its Toxicity In Vivo

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 186
Author(s):  
Maria Natalia Calienni ◽  
Daniela Maza Vega ◽  
C. Facundo Temprana ◽  
María Cecilia Izquierdo ◽  
David E. Ybarra ◽  
...  
Keyword(s):  
Side Effects ◽  
Water Solubility ◽  
Polymeric Micelles ◽  
Skin Penetration ◽  
Distribution Volume ◽  
And Performance ◽  
Oral Doses ◽  
Advanced Basal Cell Carcinoma

Vismodegib is a first-in-class inhibitor for advanced basal cell carcinoma treatment. Its daily oral doses present a high distribution volume and several side effects. We evaluated its skin penetration loaded in diverse nanosystems as potential strategies to reduce side effects and drug quantities. Ultradeformable liposomes, ethosomes, colloidal liquid crystals, and dendrimers were able to transport Vismodegib to deep skin layers, while polymeric micelles failed at this. As lipidic systems were the most effective, we assessed the in vitro and in vivo toxicity of Vismodegib-loaded ultradeformable liposomes, apoptosis, and cellular uptake. Vismodegib emerges as a versatile drug that can be loaded in several delivery systems for topical application. These findings may be also useful for the consideration of topical delivery of other drugs with a low water solubility.

scholarly journals Synthesis and Characterization of Polyvinylpyrrolidone-Modified ZnO Quantum Dots and Their In Vitro Photodynamic Tumor Suppressive Action

2021 ◽  
Vol 22 (15) ◽  
pp. 8106
Author(s):  
Tianming Song ◽  
Yawei Qu ◽  
Zhe Ren ◽  
Shuang Yu ◽  
Mingjian Sun ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Photodynamic Therapy ◽  
Inhibitory Effect ◽  
Therapeutic Effects ◽  
In Vivo Experiments ◽  
Potential Applications ◽  
Zno Quantum Dots ◽  
Zno Qds

Despite the numerous available treatments for cancer, many patients succumb to side effects and reoccurrence. Zinc oxide (ZnO) quantum dots (QDs) are inexpensive inorganic nanomaterials with potential applications in photodynamic therapy. To verify the photoluminescence of ZnO QDs and determine their inhibitory effect on tumors, we synthesized and characterized ZnO QDs modified with polyvinylpyrrolidone. The photoluminescent properties and reactive oxygen species levels of these ZnO/PVP QDs were also measured. Finally, in vitro and in vivo experiments were performed to test their photodynamic therapeutic effects in SW480 cancer cells and female nude mice. Our results indicate that the ZnO QDs had good photoluminescence and exerted an obvious inhibitory effect on SW480 tumor cells. These findings illustrate the potential applications of ZnO QDs in the fields of photoluminescence and photodynamic therapy.

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