Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited

2009 ◽  
Author(s):  
Steffen Rüttinger ◽  
Peter Kapusta ◽  
Matthias Patting ◽  
Michael Wahl ◽  
Rainer Macdonald
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Dilution Series

scholarly journals Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2972 ◽  
Author(s):  
Takuhiro Otosu ◽  
Shoichi Yamaguchi
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Single Molecule Level ◽  
Fluorescence Correlation ◽  
Promising Tool ◽  
Molecular Ruler

We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime is sensitive to the microenvironment and can be a “molecular ruler” when combined with FRET. Utilization of fluorescence lifetime in 2D FLCS thus enables us to quantify the inhomogeneity of the system and the interconversion dynamics among different species with a higher time resolution than other single-molecule techniques. Recent applications of 2D FLCS to various biological systems demonstrate that 2D FLCS is a unique and promising tool to quantitatively analyze the microsecond conformational dynamics of macromolecules at the single-molecule level.

Tracking structural transitions of bovine serum albumin in surfactant solutions by fluorescence correlation spectroscopy and fluorescence lifetime analysis

Soft Matter ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. 2512-2518 ◽  
Author(s):  
Xuzhu Zhang ◽  
Andrzej Poniewierski ◽  
Sen Hou ◽  
Krzysztof Sozański ◽  
Agnieszka Wisniewska ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Bovine Serum ◽  
Structural Changes ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Surfactant Solutions ◽  
Lifetime Analysis

Sudden structural changes of BSA in surfactant solutions are observed from FCS curves.

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