High-resolution wavefront correction in multiphoton microscopy

2009 ◽  
Author(s):  
G. Hall ◽  
M. Ren ◽  
W. B. Amos ◽  
K. W. Eliceiri ◽  
J. G. White
Keyword(s):  
High Resolution ◽  
Multiphoton Microscopy ◽  
Wavefront Correction

High-Resolution Correction of Arbitrary Wavefront Aberration Using Liquid Crystal Spatial Light Modulator

2011 ◽  
Vol 121-126 ◽  
pp. 877-881
Author(s):  
Hong Xin Zhang ◽  
Xiao Xi Xu
Keyword(s):  
Liquid Crystal ◽  
High Resolution ◽  
Spatial Light Modulator ◽  
Low Cost ◽  
Phase Conjugation ◽  
Astronomical Observation ◽  
Wavefront Aberration ◽  
Strehl Ratio ◽  
Light Modulator ◽  
Wavefront Correction

Wavefront correction plays significant role in some fields like astronomical observation, laser processing and medical imaging, etc. Liquid crystal spatial light modulator ( LC SLM) is an ideal device for high-resolution wavefront correction because of its low cost, low consumption, large number of pixels and independent programming control of each unit. It is researched experimentally that LC SLM is used as a wavefront correction device and corrects arbitrary wavefront aberration. Wavefront correction is performed based on phase conjugation and periodic phase modulation with modulo-2π. The experimental results show that the PV value of the irregular wavefront aberration is 1.56λ, RMS value is 0.25 and Strehl ratio is 0.08 before correction, but the PV value of the residual aberration is reduced to 0.26λ, RMS value is 0.02 and Strehl ratio is increased to 0.97 which is approximated diffraction limit after correction. It is proved to be feasible and effective that LC SLM is used to the high-precision and high-resolution wavefront correction.

High-resolution multiphoton microscopy with a low-power continuous wave laser pump

2018 ◽  
Vol 43 (4) ◽  
pp. 699 ◽  
Author(s):  
Xiang-Dong Chen ◽  
Shen Li ◽  
Bo Du ◽  
Yang Dong ◽  
Ze-Hao Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Low Power ◽  
Continuous Wave ◽  
Multiphoton Microscopy ◽  
Continuous Wave Laser ◽  
Laser Pump ◽  
Wave Laser

Multiparametric high-resolution imaging of barley embryos by multiphoton microscopy and magnetic resonance micro-imaging

2007 ◽  
Vol 70 (5) ◽  
pp. 426-432 ◽  
Author(s):  
Martin Stark ◽  
Bertram Manz ◽  
Alexander Ehlers ◽  
Markus Küppers ◽  
Iris Riemann ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
High Resolution ◽  
Multiphoton Microscopy ◽  
High Resolution Imaging ◽  
Resolution Imaging

scholarly journals Non-invasive imaging of tau-targeted probe uptake by whole brain multi-spectral optoacoustic tomography

2021 ◽  
Author(s):  
Patrick Vagenknecht ◽  
Maiko Ono ◽  
Artur Luzgin ◽  
Bin Ji ◽  
Makoto Higuchi ◽  
...  
Keyword(s):  
High Resolution ◽  
Progressive Supranuclear Palsy ◽  
Brain Imaging ◽  
Ex Vivo ◽  
Multiphoton Microscopy ◽  
Corticobasal Degeneration ◽  
Alzheimers Disease ◽  
Whole Brain ◽  
Optoacoustic Tomography ◽  
Non Invasive

Aim: Abnormal tau accumulation plays an important role in tauopathy diseases such as Alzheimers disease and Frontotemporal dementia. There is a need for high-resolution imaging of tau deposits at the whole brain scale in animal models. Here, we demonstrate non-invasive whole brain imaging of tau-targeted PBB5 probe in P301L model of 4-repeat tau at 130 μm resolution using volumetric multi-spectral optoacoustic tomography (vMSOT). Methods: The binding properties of a panel of imaging probes to amyloid-β, 4-repeat K18 tau fibrils were assessed by using Thioflavin T assay and surface plasmon resonance assay. We identified the probe PBB5 suitable for vMSOT tau imaging. The imaging performance was first evaluated using postmortem human brain tissues from patients with Alzheimers disease, corticobasal degeneration and progressive supranuclear palsy. Concurrent vMSOT and epi-fluorescence imaging of in vivo PBB5 targeting (i.v.) was performed in P301L and non-transgenic littermate mice. Ex vivo measurements on excised brains along with multiphoton microscopy and immunofluorescence staining of tissue sections were performed for validation. The spectrally-unmixed vMSOT data was registered with MRI atlas for volume-of-interest analysis. Results: PBB5 showed specific binding to recombinant K18 tau fibrils, AD brain tissue homogenate by competitive binding against [11C]PBB3 and to tau deposits (AT-8 positive) in post-mortem corticobasal degeneration and progressive supranuclear palsy brain. i.v. administration of PBB5 in P301L mice led to retention of the probe in tau-laden cortex and hippocampus in contrast to wild-type animals, as also confirmed by ex vivo vMSOT, epi-fluorescence and multiphoton microscopy results. Conclusion: vMSOT with PBB5 facilitates novel 3D whole brain imaging of tau in P301L animal model with high-resolution for future mechanistic studies and monitoring of putative treatments targeting tau.

Recent advances in multiphoton microscopy combined with nanomaterials in the field of disease evolution and clinical applications to liver cancer

Nanoscale ◽  
2019 ◽  
Vol 11 (42) ◽  
pp. 19619-19635 ◽  
Author(s):  
Hongxin Lin ◽  
Taojian Fan ◽  
Jian Sui ◽  
Guangxing Wang ◽  
Jianxin Chen ◽  
...  
Keyword(s):  
High Resolution ◽  
Liver Cancer ◽  
Multiphoton Microscopy ◽  
Clinical Applications ◽  
Label Free ◽  
Clinical Tool ◽  
Low Light ◽  
Disease Evolution ◽  
Recent Advances ◽  
Deep Imaging

Multiphoton microscopy (MPM) is expected to become a powerful clinical tool, with its unique advantages of being label-free, high resolution, deep imaging depth, low light photobleaching and low phototoxicity.

scholarly journals Improving Multiphoton Microscopy by Combining Spherical Aberration Patterns and Variable Axicons

Photonics ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 573
Author(s):  
Juan M. Bueno ◽  
Geovanni Hernández ◽  
Martin Skorsetz ◽  
Pablo Artal
Keyword(s):  
High Resolution ◽  
Spherical Aberration ◽  
Multiphoton Microscopy ◽  
Biological Tissues ◽  
Optical Sectioning ◽  
Established Method ◽  
Non Invasive ◽  
Microscopy Images ◽  
Better Than

Multiphoton (MP) microscopy is a well-established method for the non-invasive imaging of biological tissues. However, its optical sectioning capabilities are reduced due to specimen-induced aberrations. Both the manipulation of spherical aberration (SA) and the use of axicons have been reported to be useful techniques to bypass this limitation. We propose the combination of SA patterns and variable axicons to further improve the quality of MP microscopy images. This approach provides enhanced images at different depth locations whose quality is better than those corresponding to the use of SA or axicons separately. Thus, the procedure proposed herein facilitates the visualization of details and increases the depth observable at high resolution.

High-speed, high-resolution mesoscopic multiphoton microscopy of human skin (Conference Presentation)

2020 ◽  
Author(s):  
Alexander Fast ◽  
Anand Ganesan ◽  
Kristen Kelly ◽  
Christopher Zachary ◽  
Mihaela Balu
Keyword(s):  
High Resolution ◽  
Human Skin ◽  
High Speed ◽  
Multiphoton Microscopy
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