DNA electrochemical biosensor of methylene blue as the hybridization indicator

2007 ◽  
Author(s):  
Jimei Zhang ◽  
Wei Tong ◽  
Zhao Dai ◽  
Shichao Xu ◽  
Ning Guo ◽  
...  
2011 ◽  
Vol 298 ◽  
pp. 128-134
Author(s):  
Ai Chun Zhang ◽  
Cun Zhou

In this paper, a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology was reported. Gold nanoparticles were firstly immobilized on chitosan modified gold electrode by large amino of chitosan because of strong electrostatic adsorption effect between amino and gold atom, and thoil group modified DNA (SH-ssDNA) probe sequence was then self-assembled onto the electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied to investigate the electrochemical behavior of different modified electrodes with methylene blue as hybrid indicator. The results in optimization experiment condition show that: The peak current difference value pre and post hybridization was linearly related to the logatithmic value of the target DNA concentration ranging from 10-8~10-5mol/L.A detection limit of 3.55×10-9mol/L can be estimated.


2021 ◽  
pp. 107801
Author(s):  
José M. R. Flauzino ◽  
Rafaela C. S. Peres ◽  
Lívia M. Alves ◽  
Jussara G. Vieira ◽  
Júlia G. dos Santos ◽  
...  

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.


2016 ◽  
Vol 80 ◽  
pp. 411-417 ◽  
Author(s):  
Xiong-Wei Xu ◽  
Xiu-Hua Weng ◽  
Chang-Lian Wang ◽  
Wei-Wei Lin ◽  
Ai-Lin Liu ◽  
...  

2020 ◽  
Vol 56 (49) ◽  
pp. 6636-6639
Author(s):  
Haobo Sun ◽  
Yunliang Qiu ◽  
Yajie Lu ◽  
Jinming Kong ◽  
Xueji Zhang

In this paper, an ultrasensitive, highly selective and green electrochemical biosensor for quantifying DNA sequences (aM DNA) based on a MnTBAP catalyst for AGET ATRP reaction is proposed.


2014 ◽  
Vol 26 (6) ◽  
pp. 1304-1311 ◽  
Author(s):  
Inês B. Santarino ◽  
Severino Carlos B. Oliveira ◽  
Ana Maria Oliveira-Brett

2015 ◽  
Vol 652 ◽  
pp. 225-233 ◽  
Author(s):  
Yanqin Yang ◽  
Mengmeng Kang ◽  
Shaoming Fang ◽  
Minghua Wang ◽  
Linghao He ◽  
...  

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