Modeling of optical quadrature microscopy for imaging mouse embryos

2008 ◽  
Author(s):  
William C. Warger II ◽  
Charles A. DiMarzio
Keyword(s):  
Mouse Embryos ◽  
Optical Quadrature Microscopy

scholarly journals Determination of the Number of Cells in Preimplantation Embryos by Using Noninvasive Optical Quadrature Microscopy in Conjunction with Differential Interference Contrast Microscopy

2007 ◽  
Vol 13 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Judith A. Newmark ◽  
William C. Warger II ◽  
ChihChing Chang ◽  
Gustavo E. Herrera ◽  
Dana H. Brooks ◽  
...  
Keyword(s):  
Light Exposure ◽  
Mouse Embryos ◽  
Cell Counting ◽  
Preimplantation Embryos ◽  
Human Embryos ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Number Of Cells ◽  
Optical Quadrature Microscopy

The number of cells in a preimplantation embryo is directly correlated to the health and viability of the embryo. There are currently no methods to count the number of cells in late-stage preimplantation embryos noninvasively. We assessed the ability of optical quadrature microscopy (OQM) to count the number of cells in mouse preimplantation embryos noninvasively. First, to test for possible light toxicity, we exposed two-cell mouse embryos to OQM and differential interference contrast (DIC) microscopy and assessed their ability to develop to the blastocyst stage. We found no inhibition of development from either mode of microscopy for up to 2 h of light exposure. We also imaged eight-cell to morula stage mouse preimplantation embryos by OQM nd developed two methods for counting the number of cells. The contour signature method (CSM) used OQM images alone and the phase subtraction method (PSM) used both OQM and DIC images. We compared both methods to standard cell counting techniques and found that the PSM was superior to all other noninvasive cell counting methods. Our work on mouse embryos should be applicable to human embryos. The ability to correctly count the number of cells in human preimplantation embryos could lead to the transfer of fewer embryos in in vitro fertilization (IVF) clinics and consequently a lower rate of high-risk multiple-infant births.

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

1972 ◽  
Vol 30 ◽  
pp. 268-269
Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko
Keyword(s):  
Hela Cells ◽  
Human Adenovirus ◽  
Mouse Embryos ◽  
Equilibrium Density ◽  
Cell Stage ◽  
Virus Attachment ◽  
Preimplantation Mouse Embryos ◽  
Embryo Culture Medium ◽  
Preimplantation Mouse ◽  
Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

Preplate neurons in embryonic mouse cortex: Ultrastructural observations using photoconversion of the fluorescent lipophilic dye dil

1992 ◽  
Vol 50 (1) ◽  
pp. 906-907
Author(s):  
Linda C. Hassinger ◽  
James E. Crandall
Keyword(s):  
Mouse Embryos ◽  
Embryonic Mouse ◽  
Cortical Afferents ◽  
Mouse Cortex ◽  
Carbocyanine Dye ◽  
Increased Sensitivity

We have begun to look directly at small numbers of afferent axons to early generated neurons that form the preplate in the developing mouse cortex. The carbocyanine dye Dil (1’1, dioctadecyl-3,3,3’3’-tetramethyl-indocarbocyanine) has proved especially useful for this goal. DiI labels axons and their terminals with greater sensitivity and without some of the disadvantages of axon filling with HRP. The increased sensitivity provided by labeling embryonic axons with DiI has given us new insights into the development of cortical afferents. For instance, we reported originally that afferents from the thalamus were present below the cortex as early as embryonic day 15 (E15) based on HRP injections into mouse embryos. By using DiI placements into the thalamus in aldehyde-fixed brains, we now know that thalamic fibers reach the cortex 24 hrs earlier.

Localization of Intracisternal a Particle Antigens in Neoplastic Cells and Preimplantation Mouse Embryos

1980 ◽  
Vol 38 ◽  
pp. 696-697
Author(s):  
Thomas T.F. Huang ◽  
Patricia G. Calarco
Keyword(s):  
Endoplasmic Reticulum ◽  
Normal Development ◽  
Mouse Embryos ◽  
Neoplastic Cells ◽  
Large Numbers ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Intracisternal A Particle ◽  
Normal Feature

The stage specific appearance of a retravirus, termed the Intracisternal A particle (IAP) is a normal feature of early preimplantation development. To date, all feral and laboratory strains of Mus musculus and even Asian species such as Mus cervicolor and Mus pahari express the particles during the 2-8 cell stages. IAP form by budding into the endoplasmic reticulum and appear singly or as groups of donut-shaped particles within the cisternae (fig. 1). IAP are also produced in large numbers in several neoplastic cells such as certain plasmacytomas and rhabdomyosarcomas. The role of IAP, either in normal development or in neoplastic behavior, is unknown.

Effect of blastocyst artificial collapse prior to vitrification on pluripotency-specific genes expression in mouse embryos

2014 ◽  
Author(s):  
Mojtaba Dashtizad ◽  
Mehdi Shamsara ◽  
Morteza Daliri ◽  
Ghazaleh Zandi ◽  
Parisa Fathalizadeh ◽  
...  
Keyword(s):  
Mouse Embryos ◽  
Genes Expression

Diabetic environment and genetic predisposition as causes of congenital malformations in NOD mouse embryos

Diabetes ◽  
1991 ◽  
Vol 40 (10) ◽  
pp. 1245-1250 ◽  
Author(s):  
H. Otani ◽  
O. Tanaka ◽  
R. Tatewaki ◽  
H. Naora ◽  
T. Yoneyama
Keyword(s):  
Genetic Predisposition ◽  
Congenital Malformations ◽  
Mouse Embryos ◽  
Nod Mouse

PGE2 prevents anomalies induced by hyperglycemia or diabetic serum in mouse embryos

Diabetes ◽  
1992 ◽  
Vol 41 (12) ◽  
pp. 1644-1650 ◽  
Author(s):  
M. P. Goto ◽  
A. S. Goldman ◽  
M. R. Uhing
Keyword(s):  
Mouse Embryos
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