Wide-field time-correlated single photon counting imaging for luminescence microscopy

2008 ◽  
Author(s):  
Nicolas Sergent ◽  
James A. Levitt ◽  
Mark A. Green ◽  
Klaus Suhling
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Wide Field ◽  
Luminescence Microscopy ◽  
Single Photon Counting

scholarly journals UVscope and its application aboard the ASTRI-Horn telescope

2021 ◽  
Author(s):  
Maria Concetta Maccarone ◽  
Giovanni La Rosa ◽  
Osvaldo Catalano ◽  
Salvo Giarrusso ◽  
Alberto Segreto ◽  
...  
Keyword(s):  
Gamma Ray ◽  
Single Photon ◽  
Photon Counting ◽  
Light Flux ◽  
Cosmic Ray ◽  
High Energy ◽  
Wide Field ◽  
High Energy Astrophysics ◽  
Single Photon Counting ◽  
Photon Counting Mode

AbstractUVscope is an instrument, based on a multi-pixel photon detector, developed to support experimental activities for high-energy astrophysics and cosmic ray research. The instrument, working in single photon counting mode, is designed to directly measure light flux in the wavelengths range 300-650 nm. The instrument can be used in a wide field of applications where the knowledge of the nocturnal environmental luminosity is required. Currently, one UVscope instrument is allocated onto the external structure of the ASTRI-Horn Cherenkov telescope devoted to the gamma-ray astronomy at very high energies. Being co-aligned with the ASTRI-Horn camera axis, UVscope can measure the diffuse emission of the night sky background simultaneously with the ASTRI-Horn camera, without any interference with the main telescope data taking procedures. UVscope is properly calibrated and it is used as an independent reference instrument for test and diagnostic of the novel ASTRI-Horn telescope.

scholarly journals Development of new photon-counting detectors for single-molecule fluorescence microscopy

2013 ◽  
Vol 368 (1611) ◽  
pp. 20120035 ◽  
Author(s):  
X. Michalet ◽  
R. A. Colyer ◽  
G. Scalia ◽  
A. Ingargiola ◽  
R. Lin ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Single Photon ◽  
Photon Counting ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Large Area ◽  
Single Molecule Fluorescence ◽  
Single Photon Counting ◽  
Single Molecule Fluorescence Microscopy

Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular, they use different detectors, which share some requirements but also have major technical differences. Currently, two types of detectors best fulfil the needs of each approach: single-photon-counting avalanche diodes (SPADs) for point-like detection, and electron-multiplying charge-coupled devices (EMCCDs) for wide field detection. However, there is room for improvements in both cases. The first configuration suffers from low throughput owing to the analysis of data from a single location. The second, on the other hand, is limited to relatively low frame rates and loses the benefit of single-photon-counting approaches. During the past few years, new developments in point-like and wide field detectors have started addressing some of these issues. Here, we describe our recent progresses towards increasing the throughput of single-molecule fluorescence spectroscopy in solution using parallel arrays of SPADs. We also discuss our development of large area photon-counting cameras achieving subnanosecond resolution for fluorescence lifetime imaging applications at the single-molecule level.

Estimation of the global occurrence rate of elves with Mini-EUSO

2021 ◽  
Author(s):  
Matteo Battisti ◽  
Enrico Arnone ◽  
Mario Bertaina ◽  
Marco Casolino ◽  
Laura Marcelli ◽  
...  
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Space Station ◽  
Field Of View ◽  
Occurrence Rate ◽  
Wide Field ◽  
Single Photon Counting ◽  
Wide Range ◽  
Fluorescence Light ◽  
Atmospheric Phenomena

<p>Elves are the most common type of transient luminous events, with estimates of their global occurrence rate ranging from a few to a few tens per minute. Here, we present the first derivation of the global occurrence rate of elves from Mini-EUSO observations. Mini-EUSO is a wide field of view, space-based telescope operating from a nadir-facing UV-transparent window in the Russian Zvezda module on the International Space Station. It observes the Earth’s atmosphere in the UV band with a spatial resolution of about 6.3 km and a temporal resolution of 2.5 μs. Its optical system made of two 25 cm diameter Fresnel lenses focuses the light into a square array of 48x48 pixels, each pixel being capable of single photon counting. Originally designed to detect the faint fluorescence light produced by extensive air showers induced by extreme energy cosmic rays, it was shown to be capable of detecting a wide range of atmospheric phenomena, including elves. Elves are dynamically traced by Mini-EUSO in their horizontally expanding, fast donut-shaped light emissions and can therefore be unequivocally identified. Mini-EUSO can usually detect elves whose center is just outside the field of view, following the expansion of the ring for hundreds of microseconds. Combining the number of detected elves with consideration of the time and geometries, it is possible to derive a first estimate of their global occurrence rate with Mini-EUSO, and to compare it to the literature. </p>

Multiparametric Time-Correlated Single Photon Counting Luminescence Microscopy

2019 ◽  
Vol 84 (S1) ◽  
pp. 51-68
Author(s):  
V. I. Shcheslavskiy ◽  
M. V. Shirmanova ◽  
A. Jelzow ◽  
W. Becker
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Luminescence Microscopy ◽  
Single Photon Counting

scholarly journals Picosecond wide-field time-correlated single photon counting fluorescence microscopy with a delay line anode detector

2016 ◽  
Vol 109 (7) ◽  
pp. 071101 ◽  
Author(s):  
Liisa M. Hirvonen ◽  
Wolfgang Becker ◽  
James Milnes ◽  
Thomas Conneely ◽  
Stefan Smietana ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Delay Line ◽  
Single Photon ◽  
Photon Counting ◽  
Wide Field ◽  
Single Photon Counting
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