Part II: morphological analysis of embryonic development following femtosecond laser manipulation

2008 ◽  
Author(s):  
V. Kohli ◽  
A. Y. Elezzabi
Keyword(s):  
Embryonic Development ◽  
Femtosecond Laser ◽  
Morphological Analysis ◽  
Laser Manipulation

scholarly journals Femtosecond Laser Manipulation of Subcellular Organelles in Living Cells

2005 ◽  
Author(s):  
Wataru Watanabe ◽  
Timoko Shimada ◽  
Kazuyoshi Itoh ◽  
Sachihiro Matsunaga ◽  
Kiichi Fukui
Keyword(s):  
Femtosecond Laser ◽  
Living Cells ◽  
Subcellular Organelles ◽  
Laser Manipulation

Femtosecond laser manipulation techniques for individual patterning of biological micro-object

2008 ◽  
Author(s):  
Yoichiroh Hosokawa ◽  
Yuqiang Jiang ◽  
Isamu Oh ◽  
Noriko Takizawa ◽  
Takayuki Uwada ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Manipulation

scholarly journals Femtosecond laser manipulation of subcellular organelles in living cells

2005 ◽  
Author(s):  
Wataru Watanabe ◽  
Tomoko Shimada ◽  
Kazuyoshi Itoh ◽  
Sachihiro Matsunaga ◽  
Kiichi Fukui
Keyword(s):  
Femtosecond Laser ◽  
Living Cells ◽  
Subcellular Organelles ◽  
Laser Manipulation

scholarly journals Regeneration in the sponge Sycon ciliatum partly mimics postlarval development

Development ◽  
2020 ◽  
Vol 147 (22) ◽  
pp. dev193714
Author(s):  
Anael Soubigou ◽  
Ethan G. Ross ◽  
Yousef Touhami ◽  
Nathan Chrismas ◽  
Vengamanaidu Modepalli
Keyword(s):  
Cell Death ◽  
Embryonic Development ◽  
High Throughput ◽  
Morphological Analysis ◽  
High Throughput Sequencing ◽  
Postembryonic Development ◽  
Developmental Pathways ◽  
Genetic Features ◽  
Dissociated Cells ◽  
Comparative Transcriptomic Analysis

ABSTRACTSomatic cells dissociated from an adult sponge can reorganize and develop into a juvenile-like sponge, a remarkable phenomenon of regeneration. However, the extent to which regeneration recapitulates embryonic developmental pathways has remained enigmatic. We have standardized and established a sponge Sycon ciliatum regeneration protocol from dissociated cells. Morphological analysis demonstrated that dissociated sponge cells follow a series of morphological events resembling postembryonic development. We performed high-throughput sequencing on regenerating samples and compared the data with that from regular postlarval development. Our comparative transcriptomic analysis revealed that sponge regeneration is as equally dynamic as embryogenesis. We found that sponge regeneration is orchestrated by recruiting pathways similar to those utilized in embryonic development. We also demonstrated that sponge regeneration is accompanied by cell death at early stages, revealing the importance of apoptosis in remodelling the primmorphs to initiate re-development. Because sponges are likely to be the first branch of extant multicellular animals, we suggest that this system can be explored to study the genetic features underlying the evolution of multicellularity and regeneration.

Microbial strain improvement with femtosecond laser manipulation

2008 ◽  
Vol 136 ◽  
pp. S349
Author(s):  
Jixian Gong ◽  
Xueming Zhao ◽  
Qirong Xing
Keyword(s):  
Femtosecond Laser ◽  
Strain Improvement ◽  
Laser Manipulation ◽  
Microbial Strain

scholarly journals Longitudinal imaging and femtosecond laser manipulation of the liver: How to generate and trace single-cell-resolved micro-damage in vivo

PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0240405
Author(s):  
Daphne E. DeTemple ◽  
Sebastian Cammann ◽  
Julia Bahlmann ◽  
Manuela Buettner ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cell ◽  
Laser Manipulation ◽  
Longitudinal Imaging
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