Optical PMMA chip for multianalyte detection

2007 ◽  
Author(s):  
F. Baldini ◽  
A. Carloni ◽  
R. Falciai ◽  
A. Giannetti ◽  
A. Mencaglia ◽  
...  
Keyword(s):  
Multianalyte Detection

Simultaneous Multianalyte Detection with a Nanometer-Scale Pore

2001 ◽  
Vol 73 (10) ◽  
pp. 2268-2272 ◽  
Author(s):  
John J. Kasianowicz ◽  
Sarah E. Henrickson ◽  
Howard H. Weetall ◽  
Baldwin Robertson
Keyword(s):  
Nanometer Scale ◽  
Multianalyte Detection

Surface-plasmon-resonance-based array biosensors for multianalyte detection

1998 ◽  
Author(s):  
Michael J. O'Brien II ◽  
Victor H. Perez-Luna ◽  
Leonard M. Tender ◽  
Mark Edmunds ◽  
Ben Lascelles ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Multianalyte Detection

scholarly journals Silicon-based Biosensors for Rapid Detection of Protein or Nucleic Acid Targets

2001 ◽  
Vol 47 (10) ◽  
pp. 1894-1900 ◽  
Author(s):  
Robert Jenison ◽  
Helen La ◽  
Ayla Haeberli ◽  
Rachel Ostroff ◽  
Barry Polisky
Keyword(s):  
Thin Film ◽  
Nucleic Acid ◽  
Interferon Γ ◽  
Wild Type ◽  
Signal To Noise ◽  
Cytokine Detection ◽  
Apparent Color ◽  
Multianalyte Detection ◽  
A Charge ◽  
Silicon Based

Abstract Background: We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets in a multiplex format. Methods: Capture probes were immobilized either passively or covalently on the optically coated surface of the biosensor. Intermolecular interactions of the immobilized capture probe with specific target molecules were transduced into a molecular thin film. Thin films were generated by enzyme-catalyzed deposition in the vicinity of the surface-bound target. The increased thickness on the surface changed the apparent color of the biosensor by altering the interference pattern of reflected light. Results: Cytokine detection was achieved in a 40-min multiplex assay. Detection limits were 4 ng/L for interleukin (IL)-6, 31 ng/L for IL1-β, and 437 ng/L for interferon-γ. In multianalyte experiments, cytokines were specifically detected with signal-to-noise ratios ranging from 15 to 80. With a modified optical surface, specificity was also demonstrated in a nucleic acid array with unambiguous discrimination of single-base changes in a 15-min assay. For homozygous wild-type and homozygous mutant samples, signal-to-noise ratios of ∼100 were observed. Heterozygous samples yielded approximately equivalent signals for wild-type and mutant capture probes. Conclusions: The thin-film biosensor allows rapid, sensitive, and specific detection of protein or nucleic acid targets in an array format with results read visually or quantified with a charge-coupled device camera. This biosensor is suited for multianalyte detection in clinical diagnostic assays.

scholarly journals Electric field induced surface-enhanced Raman spectroscopy for multianalyte detection

2015 ◽  
Vol 17 (11) ◽  
pp. 7095-7099 ◽  
Author(s):  
Sumeet Walia ◽  
Aditya K. Shah ◽  
Paul R. Stoddart ◽  
Madhu Bhaskaran ◽  
Sharath Sriram
Keyword(s):  
Raman Spectroscopy ◽  
Electric Field ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Multianalyte Detection ◽  
Sers Sensing

This work demonstrates the ability to detect and isolate an analyte from a multianalyte mixture by SERS sensing.

Optical PMMA Chip Suitable for Multianalyte Detection

2008 ◽  
Vol 8 (7) ◽  
pp. 1305-1309 ◽  
Author(s):  
Francesco Baldini ◽  
Adolfo Carloni ◽  
Ambra Giannetti ◽  
Andrea Mencaglia ◽  
Giampiero Porro ◽  
...  
Keyword(s):  
Multianalyte Detection

scholarly journals A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

2007 ◽  
Vol 53 (8) ◽  
pp. 1495-1502 ◽  
Author(s):  
Jie Wu ◽  
Feng Yan ◽  
Jinhai Tang ◽  
Chun Zhai ◽  
Huangxian Ju
Keyword(s):  
Carcinoembryonic Antigen ◽  
Tumor Markers ◽  
High Throughput ◽  
Simultaneous Detection ◽  
Detection Limits ◽  
Injection Technique ◽  
Human Choriogonadotropin ◽  
Multianalyte Detection ◽  
Multianalyte Immunoassay ◽  
Screen Printed

Abstract Background: Automated and convenient multianalyte detection with high throughput is increasingly needed in clinical diagnosis. We developed a disposable 4-by-2 array for programmed simultaneous amperometric immunoassay of 4 tumor markers. Methods: We used a screen-printed technique, 1-step immobilization method, and flow injection technique. We immobilized carcinoembryonic antigen, α-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 as model analytes in a redox mediator–grafted, biopolymer-modified, screen-printed carbon electrode array to capture corresponding horseradish peroxidase-labeled antibodies in competitive immunoreactions. The simultaneous multianalyte immunoassay was automatically carried out to amperometrically monitor the mediator-catalyzed enzymatic response to hydrogen peroxide, which decreased in proportion to the concentrations of analytes in samples. Results: The multianalyte immunosensor array had a throughput of 60 samples/h and allowed simultaneous detection of carcinoembryonic antigen, α-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 in clinical serum samples with concentrations up to 188 μg/L, 250 μg/L, 266 IU/L, and 334 kIU/L, respectively. The detection limits (limits of the blank, mean of blank plus 3 SD) were 1.1 μg/L, 1.7 μg/L, 1.2 IU/L, and 1.7 kIU/L. The inter- and intraassay imprecision (CVs) of the immunosensor arrays were <7.8% and <9.0%, respectively. The immunosensor arrays were stable for 28 days. Conclusions: This newly constructed immunosensor array provides a simple, automated, simultaneous multianalyte immunoassay with high throughput, short analytical time, and sufficiently low detection limits for clinical application. This method offers the capability of miniaturizing the multianalyte detection device.

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