scholarly journals Autofluorescence removal from fluorescence tomography data using multispectral imaging

Author(s):  
Stylianos Psycharakis ◽  
Giannis Zacharakis ◽  
Anikitos Garofalakis ◽  
Rosy Favicchio ◽  
Jorge Ripoll
Author(s):  
Stylianos Psycharakis ◽  
Giannis Zacharakis ◽  
Anikitos Garofalakis ◽  
Rosy Favicchio ◽  
Jorge Ripoll

2009 ◽  
Vol 02 (04) ◽  
pp. 353-364 ◽  
Author(s):  
MARIA SIMANTIRAKI ◽  
ROSY FAVICCHIO ◽  
STELIOS PSYCHARAKIS ◽  
GIANNIS ZACHARAKIS ◽  
JORGE RIPOLL

Even though multispectral imaging is considered very significant in biological imaging, it is only commonly used in microscopy in a 2D approach. Here, we present a Fluorescence Molecular Tomography system capable of recording simultaneously tomographic data at several spectral windows, enabling multispectral tomography. 3D reconstructed data from several spectral windows is used to construct a linear unmixing algorithm for multispectral deconvolution of overlapping fluorescence signals. The method is applied on tomographic 3D fluorescence concentration maps in tissue-mimicking phantoms, yielding absolute quantification of the concentration of each individual fluorophore. Results are compared to the case when unmixing is performed in the raw 2D data instead of the reconstructed 3D concentration map, showing greater accuracy when unmixing algorithms are applied in the reconstructed data. Both the reflection and transmission geometries are considered.


2020 ◽  
Vol 26 (S2) ◽  
pp. 1282-1284
Author(s):  
Fabricio Marin ◽  
Si Chen ◽  
Francesco De Carlo ◽  
Arthur Glowacki ◽  
Olga Antipova ◽  
...  

Author(s):  
Giannis Zacharakis ◽  
Stylianos Psycharakis ◽  
Anikitos Garofalakis ◽  
Heiko Meyer ◽  
Rosy Favicchio ◽  
...  

2020 ◽  
Author(s):  
T Ritz ◽  
J Baues ◽  
O Krenkel ◽  
P Schirmacher ◽  
T Longerich

Cell Reports ◽  
2021 ◽  
Vol 34 (5) ◽  
pp. 108709
Author(s):  
Xiaojun Wang ◽  
Hanqing Xiong ◽  
Yurong Liu ◽  
Tao Yang ◽  
Anan Li ◽  
...  

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