In vitro photodynamic effect by phthalocyanine in A549 cell line

2007 ◽  
Author(s):  
Pavla Nevrelova ◽  
Hana Kolarova ◽  
Robert Bajgar ◽  
Miroslav Strnad
Keyword(s):  
Cell Line ◽  
A549 Cell ◽  
Photodynamic Effect ◽  
A549 Cell Line

scholarly journals Synthesis, In Vitro Anticancer Activity and In Silico Study of some Benzylidene Hydrazide Derivatives

2020 ◽  
Vol 840 ◽  
pp. 277-283
Author(s):  
Melanny Ika Sulistyowaty ◽  
Galih Satrio Putra ◽  
Tutuk Budiati ◽  
Katsuyoshi Matsunami
Keyword(s):  
Cell Line ◽  
A549 Cell ◽  
In Silico ◽  
Protein Tyrosine Kinase ◽  
Human Lung ◽  
Human Lung Cancer ◽  
A549 Cell Line ◽  
Mtt Method ◽  
Lung Cancer A549 Cell

Some benzylidenehydrazides (3a-e) have been synthesized in three reaction steps from anthranilic acid in good yields, about 70% - 99%. The structures of the synthesized compounds were analyzed using spectroscopic methods. The compounds were evaluated its activity against human lung cancer, A549 cell line by MTT method and studied its molecular docking onto the protein tyrosine kinase (PDB ID: 1M17) by using Molegro® vs. 5.5. The data showed that N-(2-(2-(4-nitrobenzylidene)hydrazinecarbonyl)phenyl)benzamide (3d) which synthesized in 70% yield and has the highest activity on inhibiting the growth of A549 cell line with IC50 10.9μg/mL, which was linier with our in silico study. Compound 3d has the smallest RS value -94.44 kcal/mol, lower than selected Ligand, Erlotinib.

scholarly journals Evaluation of Anticancer Activity of Olmesartan and Ramipril On A549 Cell Line

2018 ◽  
Vol 11 (3) ◽  
pp. 1351-1357 ◽  
Author(s):  
E Gayathri ◽  
K. Punnagai ◽  
D. Darling Chellathai
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Anticancer Activity ◽  
Cell Line ◽  
A549 Cell ◽  
Enzyme Inhibitor ◽  
A549 Cell Line ◽  
Ic50 Values

Angiotensin Converting Enzyme Inhibitor (ACEI) and Angiotensin II type 1 receptor antagonist (ARBs) are the most efficient cardiovascular drugs and exhibited efficient cytostatic activity in vitro in many malignant and normal cells1.OBJECTIVE: This study aims to assess the anticancer activity of these two drugs in a dose dependant manner using A549 cell line through MTT assay and Cell cycle analysis.. MATERIALS AND METHODS: Ramipril and Olmesartan were added to A549 at various concentrations ranging from 10⁻⁶ to 10mM.The dot plot of the cytotoxicity results were used to extrapolate the IC50 values. The dot plot of flow cytometry results were used to extrapolate the DNA percentage in phases of cell cycle. The plates were read at 570 nm by using a PERCLIN ELMER (multimode reader). Measurements for concentration required for 50% inhibition was noted. RESULTS: Ramipril and Olmesartan were added to A549 at various concentrations ranging from 10⁻⁶ to 10mM.The dot plot of the cytotoxicity results were used to extrapolate the IC50 values. The dot plot of flow cytometry results were used to extrapolate the DNA percentage in phases of cell cycle.

scholarly journals Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line in vitro

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Changfu Yang ◽  
Jianzhao Niu ◽  
yadong Li ◽  
Xiaobo Wang ◽  
Chunfang Huang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
A549 Cell ◽  
Cathepsin K ◽  
A549 Cell Line

scholarly journals In vitro host immune response against Acinetobacter baumannii infection using A549 cell line

2020 ◽  
Vol 101 ◽  
pp. 128-129
Author(s):  
H.J. Mea ◽  
P. Madhavan ◽  
N.K. Palanisamy ◽  
P. Yong ◽  
E.H. Wong
Keyword(s):  
Immune Response ◽  
Acinetobacter Baumannii ◽  
Cell Line ◽  
A549 Cell ◽  
Host Immune Response ◽  
A549 Cell Line

The effect of single and combined exposures to magnetite and polymorphous silicon dioxide nanoparticles on the human A549 cell line: in vitro study

2019 ◽  
Vol 26 (31) ◽  
pp. 31752-31762 ◽  
Author(s):  
Athena Rafieepour ◽  
Mansour R. Azari ◽  
Fariba Khodagholi ◽  
Jalal Pourahmad Jaktaji ◽  
Yadollah Mehrabi ◽  
...  
Keyword(s):  
Silicon Dioxide ◽  
Cell Line ◽  
A549 Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
A549 Cell Line ◽  
Silicon Dioxide Nanoparticles ◽  
Combined Exposures ◽  
Human A549

scholarly journals In vitro Assay and Study Interaction of Uncaria gambir (Hunter) Roxb. as Anti-fibrotic Activity Against A549 Cell Line

2020 ◽  
Vol 12 (6) ◽  
pp. 1232-1240
Author(s):  
Desdiani Desdiani ◽  
Iris Rengganis ◽  
Samsuridjal Djauzi ◽  
Agus Setiyono ◽  
Mohamad Sadikin ◽  
...  
Keyword(s):  
Cell Line ◽  
A549 Cell ◽  
In Vitro Assay ◽  
A549 Cell Line ◽  
Vitro Assay

scholarly journals Cannabis compounds have anti-inflammatory activity in lung epithelial cells but pro-inflammatory activity in macrophages while increasing phagocytosis in vitro

2020 ◽  
Author(s):  
Seegehalli M Anil ◽  
Nurit Shalev ◽  
Ajjampura C Vinayaka ◽  
Stalin Nadarajan ◽  
Dvory Namdar ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Line ◽  
A549 Cell ◽  
Alveolar Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Inflammatory Activity ◽  
A549 Cell Line ◽  
Alveolar Epithelial ◽  
Anti Inflammatory

Abstract Cannabis sativa is widely used for medical purposes and has anti-inflammatory activity. The purpose of this study was to examine the anti-inflammatory activity of cannabis on markers of immune responses associated with Coronavirus disease 2019 (COVID-19) inflammation. An extract fraction from C. sativa Arbel strain (FCBD) substantially reduced dose dependently interleukin (IL) 6 and 8 levels in an alveolar epithelial (A549) cell line. FCBD contained cannabidiol (CBD), cannabigerol (CBG) and tetrahydrocannabivarin (THCV), and multiple terpenes. Treatments with FCBD and phytocannabinoid standards that compose FCBD (FCBD:std) reduced IL-6, IL-8, C-C Motif Chemokine Ligands (CCLs) 2 and 7, and angiotensin I converting enzyme 2 (ACE2) expression in the A549 cell line. Treatment with FCBD induced macrophages (differentiated KG1 cell line) polarization and phagocytosis in vitro, and increased CD36 and type II receptor for the Fc region of IgG (FcγRII) expression. FCBD treatment also substantially increased IL-6 and IL-8 expression in macrophages. FCBD:std, while maintaining the anti-inflammatory activity in alveolar epithelial cells, led to reduced phagocytosis and pro-inflammatory IL secretion in macrophages in comparison to FCBD. The phytocannabinoid mixture may show superior activity versus cannabis fraction for reduction of lung inflammation, yet there is a need of caution in proposing cannabis as treatment for COVID-19.

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