Holographic optical tweezers combined with a microfluidic device for exposing cells to fast environmental changes

2007 ◽  
Author(s):  
Emma Eriksson ◽  
Jan Scrimgeour ◽  
Jonas Enger ◽  
Mattias Goksör
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Environmental Changes ◽  
Holographic Optical Tweezers

A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning

Lab on a Chip ◽  
2010 ◽  
Vol 10 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Emma Eriksson ◽  
Kristin Sott ◽  
Fredrik Lundqvist ◽  
Martin Sveningsson ◽  
Jan Scrimgeour ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Environmental Changes ◽  
Single Cells ◽  
Cell Selection

Optical force sensor array in a microfluidic device based on holographic optical tweezers

Lab on a Chip ◽  
2009 ◽  
Vol 9 (5) ◽  
pp. 661 ◽  
Author(s):  
Kai Uhrig ◽  
Rainer Kurre ◽  
Christian Schmitz ◽  
Jennifer E. Curtis ◽  
Tamás Haraszti ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Sensor Array ◽  
Force Sensor ◽  
Optical Force ◽  
Holographic Optical Tweezers

scholarly journals Measurement of dynamic membrane mechanosensation using optical tweezers

2021 ◽  
Author(s):  
Xuanling Li ◽  
Xing Liu ◽  
Xiaoyu Song ◽  
Yinmei Li ◽  
Ming Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Environmental Changes ◽  
Quantitative Measure ◽  
Relaxation Curve ◽  
Membrane Dynamics ◽  
Molecular Organization ◽  
Cytoskeletal Remodeling ◽  
Relaxation Curves ◽  
Membrane Tether

Abstract Many cellular processes are orchestrated by dynamic changes in the plasma membrane to form membrane projections and endocytic vesicles in response to extracellular environmental changes. Our previous studies show that ARF6-ACAP4-ezrin signaling regulates membrane dynamics and curvature in response to EGF stimulation. However, there is no quantitative measurement to relate molecular organization of membrane cytoskeletal remodeling to stimulus-elicited mechanosensation on the plasma membrane. Optical tweezers is a powerful tool in the study of membrane tension. Comparing to pulling out an entire membrane tether at one time, the step-like method is more efficient because multiple relaxation curves can be obtained from one membrane tether. Fewer models describe relaxation curves to characterize mechanical properties of cell membrane. Here we establish a new method to measure the membrane relaxation curve of HeLa cells judged by the relationship between membrane tether diameter and tensions. We obtained effective viscosities and static tensions by fitting relaxation curves to our model. We noticed the delicate structure of relaxation curves contains information of cytoskeletal remodeling and lateral protein diffusion. Our study established a quantitative measure to characterize the mechanosensation of epithelial cells in response to stimulus-elicited membrane dynamics.

Moving average process underlying the holographic–optical–tweezers experiments

2014 ◽  
Vol 53 (10) ◽  
pp. B254 ◽  
Author(s):  
Jakub Ślęzak ◽  
Sławomir Drobczyński ◽  
Karina Weron ◽  
Jan Masajada
Keyword(s):  
Optical Tweezers ◽  
Moving Average ◽  
Average Process ◽  
Moving Average Process ◽  
Holographic Optical Tweezers

scholarly journals Precise, contactless measurements of the surface tension of picolitre aerosol droplets

2016 ◽  
Vol 7 (1) ◽  
pp. 274-285 ◽  
Author(s):  
Bryan R. Bzdek ◽  
Rory M. Power ◽  
Stephen H. Simpson ◽  
Jonathan P. Reid ◽  
C. Patrick Royall
Keyword(s):  
Surface Tension ◽  
Optical Tweezers ◽  
Holographic Optical Tweezers ◽  
Contactless Measurements

Precise measurements of the surface tension and viscosity of airborne picolitre droplets can be accomplished using holographic optical tweezers.

Aberration correction in holographic optical tweezers using a high-order optical vortex: publisher’s note

2018 ◽  
Vol 57 (17) ◽  
pp. 4857
Author(s):  
Yansheng Liang ◽  
Yanan Cai ◽  
Zhaojun Wang ◽  
Ming Lei ◽  
Zhiliang Cao ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Optical Vortex ◽  
High Order ◽  
Aberration Correction ◽  
Holographic Optical Tweezers

Wavefront engineering and holographic optical tweezers

2015 ◽  
pp. 319-344
Author(s):  
Philip Jones ◽  
Onofrio Marago ◽  
Giovanni Volpe
Keyword(s):  
Optical Tweezers ◽  
Holographic Optical Tweezers

scholarly journals Assembly of 3-dimensional structures using programmable holographic optical tweezers

2004 ◽  
Vol 12 (22) ◽  
pp. 5475 ◽  
Author(s):  
Gavin Sinclair ◽  
Pamela Jordan ◽  
Johannes Courtial ◽  
Miles Padgett ◽  
Jon Cooper ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
3 Dimensional ◽  
Holographic Optical Tweezers

Tomographic phase microscopy with 180° rotation of live cells in suspension by holographic optical tweezers

2015 ◽  
Vol 40 (8) ◽  
pp. 1881 ◽  
Author(s):  
Mor Habaza ◽  
Barak Gilboa ◽  
Yael Roichman ◽  
Natan T. Shaked
Keyword(s):  
Optical Tweezers ◽  
Live Cells ◽  
Phase Microscopy ◽  
Holographic Optical Tweezers
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