Investigation of human white blood cells: application to scanning flow cytometry

2006 ◽  
Author(s):  
Valery A. Loiko ◽  
Gennady I. Ruban ◽  
Olga A. Gritsai ◽  
Alexander A. Miskevich ◽  
Svetlana M. Kosmacheva ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Cells ◽  
White Blood Cells ◽  
Human White Blood Cells

scholarly journals Classification of Human White Blood Cells Using Machine Learning for Stain‐Free Imaging Flow Cytometry

2019 ◽  
Vol 97 (3) ◽  
pp. 308-319 ◽  
Author(s):  
Maxim Lippeveld ◽  
Carly Knill ◽  
Emma Ladlow ◽  
Andrew Fuller ◽  
Louise J Michaelis ◽  
...  
Keyword(s):  
Machine Learning ◽  
Flow Cytometry ◽  
Blood Cells ◽  
White Blood Cells ◽  
Imaging Flow Cytometry ◽  
Human White Blood Cells

A new group of glucocerebrosidase isozymes found in human white blood cells

1980 ◽  
Vol 97 (3) ◽  
pp. 1103-1107 ◽  
Author(s):  
Edward I. Ginns ◽  
Roscoe O. Brady ◽  
Daniel W. Stowens ◽  
F.Scott Furbish ◽  
John A. Barranger
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Human White Blood Cells

scholarly journals Identification of phosphatidylserine-binding proteins in human white blood cells

1990 ◽  
Vol 269 (3) ◽  
pp. 723-728 ◽  
Author(s):  
M Wolf ◽  
M Baggiolini
Keyword(s):  
Protein Kinase C ◽  
Blood Cells ◽  
Binding Proteins ◽  
White Blood Cells ◽  
Human Neutrophils ◽  
Kinase C ◽  
Sds Page ◽  
Molecular Masses ◽  
Human White Blood Cells ◽  
Monocytes And Lymphocytes

Cytosol and membrane fractions from human neutrophils, monocytes, lymphocytes and platelets were separated by SDS/PAGE, blotted on to nitrocellulose and assayed for selective binding of phosphatidylserine (PS). Two PS-binding proteins with apparent molecular masses of 115 kDa and 100 kDa were identified in the cytosol of neutrophils, monocytes and lymphocytes. Corresponding bands along with other PS-binding proteins were detected in platelets in both cytosol and membrane fractions. These proteins were also found to bind protein kinase C (PKC) provided that PS was present. The 115 kDa and 100 kDa proteins (PS-p115/110) were partially purified from neutrophils and were used for the study of PS and PKC binding. The binding of PS did not require Ca2+ or Mg2+ and was inhibited by phosphatidic acid, by 1-alkyl-2-acetylphosphocholine and, to a lesser extent, by other lipids. The binding of PKC, however, was strictly PS- and Ca2(+)-dependent and seems to occur secondarily to PS binding.

Direct freezing of whole blood enables analysis of leucocyte markers by flow cytometry: a proof-of-concept study

2021 ◽  
Author(s):  
Pénélope Bourgoin ◽  
Inès Ait Belkacem ◽  
Isabelle Arnoux ◽  
Pierre-Emmanuel Morange ◽  
Fabrice Malergue
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Proof Of Concept ◽  
Fresh Blood ◽  
Blood Samples ◽  
Step Flow ◽  
Freezing Method ◽  
One Step

Aim: A new one-step flow cytometry procedure has been recently demonstrated for identifying subjects with infections, but only for fresh whole blood samples. The goal of this study was to assess its applicability on frozen samples, by proposing a new method to perform the sample freezing directly and easily. Methods: Fresh blood was tested, then frozen either directly or with dimethylsulfoxide and serum. Common markers of white blood cells as well as infection-related biomarkers were tested. Results: All percentages of leucocyte subsets and levels of infection-related biomarkers were significantly correlated between frozen and fresh samples. Conclusion: The direct freezing method enables an accurate assessment of common cellular sub-populations and of levels of important infectious biomarkers via flow cytometry.

Expression of CD80 (B7/BB-1) and CD28 in human white blood cells treated with urocanic acid

1996 ◽  
Vol 288 (10) ◽  
pp. 570-574 ◽  
Author(s):  
Jarmo K. Laihia ◽  
Jaakko Uksila ◽  
Marko Luhtala ◽  
Christer T. Jansén
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Urocanic Acid ◽  
Human White Blood Cells
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