In vitro studies of the efficiency of two-photon activation of photodynamic therapy agents

2006 ◽  
Author(s):  
Mamta Khurana ◽  
Aliaksandr Karotki ◽  
Hazel Collins ◽  
Harry L. Anderson ◽  
Brian C. Wilson
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Studies ◽  
Photon Activation ◽  
Two Photon

scholarly journals Efficacy of Toluidine Blue—Mediated Antimicrobial Photodynamic Therapy on Candida spp. A Systematic Review

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 349
Author(s):  
Rafał Wiench ◽  
Dariusz Skaba ◽  
Jacek Matys ◽  
Kinga Grzech-Leśniak
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Randomized Clinical Trials ◽  
Oral Candidiasis ◽  
In Vitro Studies ◽  
Cochrane Central Register ◽  
Antimicrobial Photodynamic Therapy ◽  
Candida Spp ◽  
Denture Stomatitis

The effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral yeast infections was examined many times in recent years. The authors of this review tried to address the question: “Should TBO (toluidine blue ortho)-mediated aPDT be considered a possible alternative treatment for oral candidiasis?”. PubMed/Medline and the Cochrane Central Register of Controlled Trials (CEN-TRAL) databases were searched from 1997 up to the 27th of October 2020 using a combination of the following keywords: (Candida OR Candidiasis oral OR Candidosis oral OR denture stomatitis) AND (toluidine blue OR photodynamic therapy OR aPDT OR photodynamic antimicrobial chemotherapy OR PACT OR photodynamic inactivation OR PDI). Animal studies or in vitro studies involving Candida albicans (C. albicans) and/or nonalbicans stain, randomized clinical trials (RCT) involving patients with oral candidiasis or denture stomatitis published solely in English language were included. Candida elimination method in animal, in vitro studies and RCT used was TBO-mediated aPDT. Exactly 393 studies were taken into consideration. Then, after analyzing titles and abstracts of said studies, 361 were excluded. Only 32 studies ended up being selected for in-depth screening, after which 21 of them were included in this study. All studies reported the antifungal effectiveness of aPDT with TBO against C. albicans and non-albicans Candida. In studies conducted with planktonic cells, only one study showed eradication of C. albicans. All others showed partial elimination and only one of them was not statistically significant. Experiments on yeast biofilms, in all cases, showed partial, statistically significant cell growth inhibition and weight reduction (a reduction in the number of cells—mainly hyphae) and the mass of extracellular polymeric substance (EPS). In vivo aPDT mediated by TBO exhibits antifungal effects against oral Candida spp.; however, its clinical effectiveness as a potent therapeutic strategy for oral yeast infections requires further investigation.

scholarly journals Biological Assessment of Laser-Synthesized Silicon Nanoparticles Effect in Two-Photon Photodynamic Therapy on Breast Cancer MCF-7 Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1462
Author(s):  
Ahmed Al-Kattan ◽  
Lamiaa M. A. Ali ◽  
Morgane Daurat ◽  
Elodie Mattana ◽  
Magali Gary-Bobo
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Silicon Nanoparticles ◽  
Therapeutic Potential ◽  
Light Stimulus ◽  
Human Cancer ◽  
Biological Assessment ◽  
Two Photon ◽  
Mcf 7

Driven by their distinctive physiological activities, biological properties and unique theranostic modalities, silicon nanoparticles (SiNPs) are one of the promising materials for the development of novel multifunctional nanoplatforms for biomedical applications. In this work, we assessed the possibility to use laser-synthesized Si NPs as photosensitizers in two-photon excited photodynamic therapy (TPE-PDT) modality. Herein, we used an easy strategy to synthesize ultraclean and monodispersed SiNPs using laser ablation and fragmentation sequences of silicon wafer in aqueous solution, which prevent any specific purification step. Structural analysis revealed the spherical shape of the nanoparticles with a narrow size distribution centered at the mean size diameter of 62 nm ± 0.42 nm, while the negative surface charge of −40 ± 0.3 mV ensured a great stability without sedimentation over a long period of time. In vitro studies on human cancer cell lines (breast and liver) and healthy cells revealed their low cytotoxicity without any light stimulus and their therapeutic potential under TPE-PDT mode at 900 nm with a promising cell death of 45% in case of MCF-7 breast cancer cells, as a consequence of intracellular reactive oxygen species release. Their luminescence emission inside the cells was clearly observed at UV-Vis region. Compared to Si nanoparticles synthesized via chemical routes, which are often linked to additional modules with photochemical and photobiological properties to boost photodynamic effect, laser-synthesized SiNPs exhibit promising intrinsic therapeutic and imaging properties to develop advanced strategy in nanomedicine field.

Antimicrobial effect of photodynamic therapy on intracanal biofilm: A systematic review of in vitro studies

2020 ◽  
Vol 32 ◽  
pp. 102025
Author(s):  
Yasmin Vendramini ◽  
Alexandre Salles ◽  
Fernando Freitas Portella ◽  
Myrian Camara Brew ◽  
Liviu Steier ◽  
...  
Keyword(s):  
Systematic Review ◽  
Photodynamic Therapy ◽  
Antimicrobial Effect ◽  
In Vitro Studies

scholarly journals Biocompatible conjugated fluorenylporphyrins for two-photon photodynamic therapy and fluorescence imaging

2019 ◽  
Vol 55 (81) ◽  
pp. 12231-12234 ◽  
Author(s):  
Limiao Shi ◽  
Christophe Nguyen ◽  
Morgane Daurat ◽  
Abdelhamid Chiheb Dhieb ◽  
Wajda Smirani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Breast Cancer Cells ◽  
Two Photon ◽  
Theranostic Applications

Three new biocompatible porphyrin-based oxygen photosensitisers were tested in vitro on breast cancer cells via 2P-PDT: one of them, 66 times more active than H2TPP, gave quite promising results for theranostic applications.

scholarly journals In Vitro Studies on Erythrosine-Based Photodynamic Therapy of Malignant and Pre-Malignant Oral Epithelial Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34475 ◽  
Author(s):  
Abhishek D. Garg ◽  
Muthiah Bose ◽  
Mohammed I. Ahmed ◽  
William A. Bonass ◽  
Simon R. Wood
Keyword(s):  
Photodynamic Therapy ◽  
Epithelial Cells ◽  
In Vitro Studies ◽  
Oral Epithelial Cells ◽  
Oral Epithelial

scholarly journals Erratum to: “In vitro studies of Photofrin® mediated photodynamic therapy on human Rhabdomyosarcoma cell line (RD)”

Laser Physics ◽  
2012 ◽  
Vol 22 (2) ◽  
pp. 477-478
Author(s):  
M. Atif ◽  
A. R. Malik ◽  
M. Fakhar-e-Alam ◽  
S. S. Hayat ◽  
S. S. Z. Zaidi ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Line ◽  
In Vitro Studies ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell

scholarly journals Multifunctional AIE Iridium (III) Photosensitizer Nanoparticles for Two-Photon-Activated Imaging and Mitochondria Targeting  Photodynamic Therapy

2021 ◽  
Author(s):  
Xuzi Cai ◽  
Kangnan Wang ◽  
Wen Ma ◽  
yuanyuan Yang ◽  
Gui Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Solubility ◽  
Redox Homeostasis ◽  
Tissue Imaging ◽  
Ros Generation ◽  
Generation Efficiency ◽  
Deep Tissue ◽  
Two Photon ◽  
Targeting Ability

Abstract Developing novel photosensitizers for deep tissue imaging and efficient photodynamic therapy (PDT) remains a challengebecause of the poor water solubility, low reactive oxygen species (ROS) generation efficiency, serve dark cytotoxicity, and weak absorption in the NIR region of conventional photosensitizers. Herein,cyclometalated iridium (III) complexes(Ir)with aggregation-induced emission (AIE) feature, high photoinduced ROS generation efficiency, two-photonexcitation, and mitochondria-targetingcapability were designed and further encapsulated into biocompatible nanoparticles (NPs).The Ir-NPs can be used to disturb redox homeostasis in vitro, result in mitochondrial dysfunction and cell apoptosis. Importantly, invivo experiments demonstrated that theIr-NPs presented obviously tumor-targeting ability, excellent antitumor effect, and low systematic dark-toxicity. Moreover, the Ir-NPs could serve as a two-photon imaging agent for deep tissue bioimaging with a penetration depth of up to 300 μm. This work presents a promising strategy for designing a clinical application of multifunctional Ir-NPs toward bioimaging and PDT.

scholarly journals A Luminescent, Water-Soluble Ir(III) Complex as a Potential Photosensitizer for Two-Photon Photodynamic Therapy

2021 ◽  
Vol 11 (24) ◽  
pp. 11596
Author(s):  
Elisabeta I. Szerb ◽  
Sharmistha Chatterjee ◽  
Massimo La Deda ◽  
Giovanna Palermo ◽  
Lucie Sancey ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Soluble ◽  
In Vitro Experiments ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Biologically Relevant ◽  
Two Photon ◽  
Fluorescence Measurements ◽  
Detection Probe

This work reports the study of two-photon induced properties of a highly luminescent cyclometalated Ir(III) complex, [Ir(ppy)2(en)] OOCCH3 (1), ppy = 2-phenylpyridine, en = ethylenediamine. Steady-state and time-resolved fluorescence measurements were performed by exciting 1 at the biologically relevant wavelength of 800 nm, whereas, the generation of singlet oxygen (1O2) was evaluated using 9,10-Anthracenediyl-bis(methylene)dimalonic acid (ABDA) as a detection probe. Preliminary in vitro experiments with U87-MG cells were performed, showing the potential of this compound as a two-photon photodynamic therapy (2P-PDT) agent at NIR wavelengths.

Effect of curcumin-mediated photodynamic therapy on Streptococcus mutans and Candida albicans: A systematic review of in vitro studies

2019 ◽  
Vol 27 ◽  
pp. 455-461 ◽  
Author(s):  
Daniele de Cassia Rodrigues Picco ◽  
Leonardo Lobo Ribeiro Cavalcante ◽  
Rayana Longo Bighetti Trevisan ◽  
Aline Evangelista Souza-Gabriel ◽  
Maria Cristina Borsatto ◽  
...  
Keyword(s):  
Systematic Review ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
In Vitro Studies
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