A computational 3D model for reconstruction of neural stem cells in bright-field time-lapse microscopy

2007 ◽  
Author(s):  
J. Degerman ◽  
E. Winterfors ◽  
J. Faijerson ◽  
T. Gustavsson
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
3D Model ◽  
Time Lapse ◽  
Bright Field ◽  
Time Lapse Microscopy

scholarly journals Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells – Tools for analyzing dynamics of cell cycle, migration, and lineage selection

Methods ◽  
2018 ◽  
Vol 133 ◽  
pp. 81-90 ◽  
Author(s):  
Katja M. Piltti ◽  
Brian J. Cummings ◽  
Krystal Carta ◽  
Ayla Manughian-Peter ◽  
Colleen L. Worne ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Cell Tracking ◽  
Time Lapse ◽  
Live Cell ◽  
Time Lapse Imaging

scholarly journals Embryonic cortical neural stem cells migrate ventrally and persist as postnatal striatal stem cells

2006 ◽  
Vol 175 (1) ◽  
pp. 159-168 ◽  
Author(s):  
Sandrine Willaime-Morawek ◽  
Raewyn M. Seaberg ◽  
Claudia Batista ◽  
Etienne Labbé ◽  
Liliana Attisano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Homeobox Gene ◽  
Time Lapse ◽  
Video Microscopy ◽  
Lineage Tracing ◽  
Cell Labeling ◽  
Germinal Zone ◽  
Direct Cell ◽  
Time Lapse Video

Embryonic cortical neural stem cells apparently have a transient existence, as they do not persist in the adult cortex. We sought to determine the fate of embryonic cortical stem cells by following Emx1IREScre; LacZ/EGFP double-transgenic murine cells from midgestation into adulthood. Lineage tracing in combination with direct cell labeling and time-lapse video microscopy demonstrated that Emx1-lineage embryonic cortical stem cells migrate ventrally into the striatal germinal zone (GZ) perinatally and intermingle with striatal stem cells. Upon integration into the striatal GZ, cortical stem cells down-regulate Emx1 and up-regulate Dlx2, which is a homeobox gene characteristic of the developing striatum and striatal neural stem cells. This demonstrates the existence of a novel dorsal-to-ventral migration of neural stem cells in the perinatal forebrain.

scholarly journals NeuriTES. Monitoring neurite changes through transfer entropy and semantic segmentation in bright-field time-lapse microscopy

Patterns ◽  
2021 ◽  
pp. 100261
Author(s):  
Arianna Mencattini ◽  
Alida Spalloni ◽  
Paola Casti ◽  
Maria Colomba Comes ◽  
Davide Di Giuseppe ◽  
...  
Keyword(s):  
Semantic Segmentation ◽  
Time Lapse ◽  
Transfer Entropy ◽  
Bright Field ◽  
Time Lapse Microscopy

scholarly journals Comparsion of Migration Pattern between Young and Senescent Mesenchymal Stem Cells in Time Lapse Microscopy

2015 ◽  
Vol 108 (2) ◽  
pp. 456a-457a
Author(s):  
Ching-Fen Jiang ◽  
Shan-hui Hsu ◽  
Ka-Pei Tsai ◽  
Jia-Yin Li
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Time Lapse ◽  
Migration Pattern ◽  
Time Lapse Microscopy

scholarly journals Drosophila neural stem cells show a unique dynamic pattern of gene expression that is influenced by environmental factors

2020 ◽  
Author(s):  
Alix Goupil ◽  
Carole Pennetier ◽  
Anthony Simon ◽  
Patricia Skorski ◽  
Allison Bardin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Environmental Changes ◽  
Gene Expression Regulation ◽  
Low Frequency ◽  
Time Lapse ◽  
Chromosome Loss ◽  
The Brain

AbstractWith the aim of developing a sensor for chromosome loss in vivo, we used the well-established GAL4/GAL80 system combined with a visual GFP marker in Drosophila. We show a low frequency of green cells in most Drosophila tissues, suggesting low aneuploidy levels. Unexpectedly, in the brain, GFP positive cells are more frequent, but in this case, they do not represent chromosome loss. Using genetic manipulations, RNA FISH and time-lapse microscopy, we uncovered a dynamic and reversible silencing of GAL80 that occurs in Drosophila neural stem cells. Further, we showed that this novel gene expression regulation is influenced by environmental changes such as temperature variations or food composition. These results have important implications for the Drosophila community, namely the possible interpretation of false positive cells in clonal experiments. Additionally, they also highlight a level of mosaicism and plasticity in the brain, consistent with possible epigenetic regulation of fly chromosomes, which is different from other organs and tissues.

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