Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells - in vitro study

2007 ◽  
Author(s):  
Wu Yue ◽  
Da-yong Song ◽  
Zhao Zhao ◽  
Shao-dong Cao ◽  
Xiang-xi Meng ◽  
...  
Keyword(s):  
Cytotoxic Effect ◽  
Glioma Cells ◽  
In Vitro Study ◽  
Monomethyl Ether ◽  
C6 Glioma ◽  
Vitro Study ◽  
C6 Glioma Cells ◽  
Hematoporphyrin Monomethyl Ether

In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells

2008 ◽  
Vol 29 (4) ◽  
pp. 229-235 ◽  
Author(s):  
Jian-hua Li ◽  
Da-yong Song ◽  
Yong-gang Xu ◽  
Zheng Huang ◽  
Wu Yue
Keyword(s):  
Glioma Cells ◽  
In Vitro Study ◽  
Monomethyl Ether ◽  
C6 Glioma ◽  
Vitro Study ◽  
C6 Glioma Cells

scholarly journals In vitro study of low intensity ultrasound combined with different doses of PDT: Effects on C6 glioma cells

2012 ◽  
Vol 5 (2) ◽  
pp. 702-706 ◽  
Author(s):  
JIAN-HUA LI ◽  
ZHI-QIANG CHEN ◽  
ZHENG HUANG ◽  
QI ZHAN ◽  
FU-BIN REN ◽  
...  
Keyword(s):  
Glioma Cells ◽  
In Vitro Study ◽  
C6 Glioma ◽  
Vitro Study ◽  
C6 Glioma Cells ◽  
Low Intensity ◽  
Low Intensity Ultrasound ◽  
Different Doses

Radiosensitivity of glioma to Gamma Knife treatment enhanced in vitro and in vivo by RNA interfering Ku70 that is mediated by a recombinant adenovirus

2010 ◽  
Vol 113 (Special_Supplement) ◽  
pp. 228-235 ◽  
Author(s):  
Qiang Jia ◽  
Yanhe Li ◽  
Desheng Xu ◽  
Zhenjiang Li ◽  
Zhiyuan Zhang ◽  
...  
Keyword(s):  
Western Blot ◽  
Gamma Knife ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells

Object The authors sought to evaluate modification of the radiation response of C6 glioma cells in vitro and in vivo by inhibiting the expression of Ku70. To do so they investigated the effect of gene transfer involving a recombinant replication-defective adenovirus containing Ku70 short hairpin RNA (Ad-Ku70shRNA) combined with Gamma Knife treatment (GKT). Methods First, Ad-Ku70shRNA was transfected into C6 glioma cells and the expression of Ku70 was measured using Western blot analysis. In vitro, phenotypical changes in C6 cells, including proliferation, cell cycle modification, invasion ability, and apoptosis were evaluated using the MTT (3′(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay, Western blot analysis, and cell flow cytometry. In vivo, parental C6 cells transfected with Ad-Ku70shRNA were implanted stereotactically into the right caudate nucleus in Sprague-Dawley rats. After GKS, apoptosis was analyzed using the TUNEL (terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling) method. The inhibitory effects on growth and invasion that were induced by expression of proliferating cell nuclear antigen and matrix metalloproteinase–9 were determined using immunohistochemical analyses. Results The expression of Ku70 was clearly inhibited in C6 cells after transfection with Ad-Ku70shRNA. In vitro following transfection, the C6 cells showed improved responses to GKT, including suppression of proliferation and invasion as well as an increased apoptosis index. In vivo following transfection of Ad-Ku70shRNA, the therapeutic efficacy of GKT in rats with C6 gliomas was greatly enhanced and survival times in these animals were prolonged. Conclusions Our data support the potential for downregulation of Ku70 expression in enhancing the radiosensitivity of gliomas. The findings of our study indicate that targeted gene therapy–mediated inactivation of Ku70 may represent a promising strategy in improving the radioresponsiveness of gliomas to GKT.

scholarly journals Investigation of characterization and cytotoxic effect of PEGylated nanoliposomal containing Melphalan on ovarian cancer: an in vitro study

2021 ◽  
Vol 16 (1) ◽  
pp. 102-116
Author(s):  
Seyedeh Masoomeh Sadat Mirnezami ◽  
Amir Heidarinasab ◽  
Azim Akbarzadeh ◽  
Mehdi Arjmand
Keyword(s):  
Ovarian Cancer ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Vitro Study

Significant correlations between atmospheric spectra according to Baumer and the in vitro incorporation of [3 H]thymidine into the nuclear DNA of C6-glioma cells

FEBS Letters ◽  
1991 ◽  
Vol 288 (1-2) ◽  
pp. 244-246 ◽  
Author(s):  
Siegfried Vogl ◽  
Georg Hoffmann ◽  
Barbara Stöpfel ◽  
Hans Baumer ◽  
Oliver Kempski ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells
Sign in / Sign up

Export Citation Format

Share Document