Optical nano-injection into cells and 3D stem cell clusters via a NIR femtosecond laser

2007 ◽  
Author(s):  
Iris Riemann ◽  
Frank Stracke ◽  
Aisada Uchugonova ◽  
Sven Martin ◽  
Rainer Bückle ◽  
...  
Keyword(s):  
Stem Cell ◽  
Femtosecond Laser ◽  
Cell Clusters

The influence of NIR femtosecond laser radiation on the viability of 3D stem cell clusters and tumor spheroids

2007 ◽  
Author(s):  
Aisada Uchugonova ◽  
Iris Riemann ◽  
Frank Stracke ◽  
Erwin Gorjup ◽  
Ronan LeHarzic ◽  
...  
Keyword(s):  
Stem Cell ◽  
Laser Radiation ◽  
Femtosecond Laser ◽  
Femtosecond Laser Radiation ◽  
Tumor Spheroids ◽  
Cell Clusters

scholarly journals Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kazufumi Sakamoto ◽  
Yoshitsune Hondo ◽  
Naoki Takahashi ◽  
Yuhei Tanaka ◽  
Rikuto Sekine ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Single Cells ◽  
Embryonic Stem ◽  
Kuramoto Model ◽  
Distribution Analysis ◽  
Cell Clusters ◽  
Overdrive Suppression ◽  
The Kuramoto Model

AbstractWe investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic-stem-cell (hES)-derived cardiomyocytes. Cardiomyocyte clusters were formed in concave agarose cultivation chambers and their beating intervals were compared with those of dispersed isolated single cells. Distribution analysis revealed that the clusters’ synchronized interbeat intervals (IBIs) were longer than the majority of those of isolated single cells, which is against the conventional faster firing regulation or “overdrive suppression.” IBI distribution of the isolated individual cardiomyocytes acquired from the beating clusters also confirmed that the clusters’ IBI was longer than those of the majority of constituent cardiomyocytes. In the complementary experiment in which cell clusters were connected together and then separated again, two cardiomyocyte clusters having different IBIs were attached and synchronized to the longer IBIs than those of the two clusters’ original IBIs, and recovered to shorter IBIs after their separation. This is not only against overdrive suppression but also mathematical synchronization models, such as the Kuramoto model, in which synchronized beating becomes intermediate between the two clusters’ IBIs. These results suggest that emergent slower synchronous beating occurred in homogeneous cardiomyocyte clusters as a community effect of spontaneously beating cells.

3D Human Adipose-Derived Stem Cell Clusters as a Model for In Vitro Fibrosis

2016 ◽  
Vol 22 (7) ◽  
pp. 679-690 ◽  
Author(s):  
Thanavel Rajangam ◽  
Min Hee Park ◽  
Sang-Heon Kim
Keyword(s):  
Stem Cell ◽  
Cell Clusters ◽  
Adipose Derived Stem Cell

Multiscale grooved titanium processed with femtosecond laser influences mesenchymal stem cell morphology, adhesion, and matrix organization

2012 ◽  
Vol 100A (11) ◽  
pp. 3108-3116 ◽  
Author(s):  
Virginie Dumas ◽  
Aline Rattner ◽  
Laurence Vico ◽  
Eric Audouard ◽  
Jean Claude Dumas ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Femtosecond Laser ◽  
Cell Morphology ◽  
Matrix Organization

In Vivo Functioning and Transplantable Mature Pancreatic Islet-Like Cell Clusters Differentiated from Embryonic Stem Cell

Pancreas ◽  
2003 ◽  
Vol 27 (2) ◽  
pp. e34-e41 ◽  
Author(s):  
Dohoon Kim ◽  
Yuanjun Gu ◽  
Michiyo Ishii ◽  
Mineko Fujimiya ◽  
Meirigeng Qi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Pancreatic Islet ◽  
Embryonic Stem ◽  
Cell Clusters

Femtosecond laser nano/micro patterning of titanium influences mesenchymal stem cell adhesion and commitment

2015 ◽  
Vol 10 (5) ◽  
pp. 055002 ◽  
Author(s):  
Virginie Dumas ◽  
Alain Guignandon ◽  
Laurence Vico ◽  
Cyril Mauclair ◽  
Ximena Zapata ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Mesenchymal Stem Cell ◽  
Femtosecond Laser ◽  
Micro Patterning

scholarly journals Effect of the distance between adherent mesenchymal stem cell and the focus of irradiation of femtosecond laser on cell replication capacity

2012 ◽  
Vol 64 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Jun Sakai ◽  
Daniel Roldán ◽  
Kosei Ueno ◽  
Hiroaki Misawa ◽  
Yoichiroh Hosokawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Femtosecond Laser ◽  
Replication Capacity ◽  
Cell Replication

A Novel Human Stem Cell Coculture System that Maintains the Survival and Function of Culture Islet-Like Cell Clusters

2008 ◽  
Vol 17 (6) ◽  
pp. 657-664 ◽  
Author(s):  
Kuo Ching Chao ◽  
Kuo Fang Chao ◽  
Cheng-Feng Chen ◽  
Shing Hwa Liu
Keyword(s):  
Stem Cell ◽  
Human Stem Cell ◽  
Cell Clusters ◽  
Coculture System ◽  
And Function
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