In vivo resolution of two near-infrared fluorophores by time-domain diffuse optical tomagraphy

2007 ◽  
Author(s):  
Walter Akers ◽  
Samuel Achilefu
Keyword(s):  
Time Domain ◽  
Near Infrared

Scanning Time-domain Optical Mammography: Detection and Characterization of Breast Tumors In Vivo

2005 ◽  
Vol 4 (5) ◽  
pp. 483-496 ◽  
Author(s):  
Herbert Rinneberg ◽  
Dirk Grosenick ◽  
K. Thomas Moesta ◽  
Jörg Mucke ◽  
Bernd Gebauer ◽  
...  
Keyword(s):  
Time Domain ◽  
Near Infrared ◽  
Strong Light ◽  
New Techniques ◽  
Optical Mammography ◽  
Scanning Time ◽  
Nir Light ◽  
Tissue Scattering

Optical mammography is one of several new techniques for breast cancer detection and characterization presently under development for clinical use that provide information other than morphologic, in particular on the biochemical and metabolic state of normal and diseased tissue. In breast tissue, scattering of red to near infrared (NIR) light dominates absorption and NIR light may penetrate several centimeters through the breast. Optical mammography avoids the use of ionizing radiation and offers the power of diffuse optical spectroscopy. However, because of strong light scattering, spatial resolution of optical mammography is generally low. The paper reviews the results of a clinical study on scanning time-domain optical mammography comprising 154 patients carrying a total of 102 carcinomas validated by histology. Ninety two of these tumors were detected in optical mammograms retrospectively and for 87 of the detected tumors optical properties and tissue parameters were derived. In addition developments on instrumentation and data analysis are covered and possible improvements of optical mammography are briefly discussed.

scholarly journals Special Issue on New Horizons in Time Domain Diffuse Optical Spectroscopy and Imaging

2020 ◽  
Vol 10 (8) ◽  
pp. 2752
Author(s):  
Yoko Hoshi
Keyword(s):  
Infrared Spectroscopy ◽  
Optical Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Time Domain ◽  
Near Infrared ◽  
Special Issue ◽  
Diffuse Optical Spectroscopy ◽  
New Horizons

In 1977, Jöbsis first described the in vivo application of near-infrared spectroscopy (NIRS) [...]

scholarly journals Time Domain Near Infrared Spectroscopy Device for Monitoring Muscle Oxidative Metabolism: Custom Probe and In Vivo Applications

Sensors ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 264 ◽  
Author(s):  
Rebecca Re ◽  
Ileana Pirovano ◽  
Davide Contini ◽  
Lorenzo Spinelli ◽  
Alessandro Torricelli
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Time Domain ◽  
Oxidative Metabolism ◽  
Near Infrared

scholarly journals Dynamic Analysis of the Blood-Brain Barrier Disruption in Experimental Stroke Using Time Domain in Vivo Fluorescence Imaging

2008 ◽  
Vol 7 (6) ◽  
pp. 7290.2008.00025 ◽  
Author(s):  
Abedelnasser Abulrob ◽  
Eric Brunette ◽  
Jacqueline Slinn ◽  
Ewa Baumann ◽  
Danica Stanimirovic
Keyword(s):  
Optical Imaging ◽  
Contrast Enhancement ◽  
Blood Brain Barrier ◽  
Time Domain ◽  
Near Infrared ◽  
Ex Vivo ◽  
Brain Barrier ◽  
Experimental Stroke ◽  
Blood Brain

The blood-brain barrier (BBB) disruption following cerebral ischemia can be exploited to deliver imaging agents and therapeutics into the brain. The aim of this study was (a) to establish novel in vivo optical imaging methods for longitudinal assessment of the BBB disruption and (b) to assess size selectivity and temporal patterns of the BBB disruption after a transient focal ischemia. The BBB permeability was assessed using in vivo time domain near-infrared optical imaging after contrast enhancement with either free Cy5.5 (1 kDa) or Cy5.5 conjugated with bovine serum albumin (BSA) (67 kDa) in mice subjected to either 60- or 20-minute transient middle cerebral artery occlusion (MCAO) and various times of reperfusion (up to 14 days). In vivo imaging observations were corroborated by ex vivo brain imaging and microscopic analyses of fluorescent tracer extravasation. The in vivo optical contrast enhancement with Cy5.5 was spatially larger than that observed with BSA-Cy5.5. Longitudinal studies after a transient 20-minute MCAO suggested a bilateral BBB disruption, more pronounced in the ipsilateral hemisphere, peaking at day 7 and resolving at day 14 after ischemia. The area differential between the BBB disruption for small and large molecules could potentially be useful as a surrogate imaging marker for assessing perinfarct tissues to which neuroprotective therapies of appropriate sizes could be delivered.

Discrimination and Quantification of Spectrally Similar Near-Infrared Probes by Time-Domain (TD) Optical Imaging in Acute Myeloid Leukemia Mouse Models.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4319-4319
Author(s):  
Emmet McCormack ◽  
Alexandre Belankov ◽  
Maja Mujic ◽  
Pierre Couture ◽  
Bjorn T. Gjertsen
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Time Domain ◽  
Spectral Properties ◽  
Near Infrared ◽  
Leukemic Cells ◽  
Whole Body ◽  
Single Wave ◽  
Optical Absorbance

Abstract The use of whole-body optical imaging in the near-infrared (NIR) spectrum (650–1100 nm) employing fluorescently labelled reagents recognising cell-specific biomarkers of leukemia has become a standard modality in preclinical models of the human disease. A particular challenge is represented by leukemic infiltrates in liver and spleen, organs with high optical absorbance. While there are increasingly impressive arrays of fluorescently labelled biomolecules available for exploitation via optical imaging, the number of commmercially availible fluorophores for NIR imaging remain limited. In particular, simultaneous imaging of disease progression and functional imaging of more specific biological processes within the same sample is complicated by the requiste for multiple filtersets for fluorophores with similar spectral properties. Subsequent “bleeding” fluorescence through filtersets is unavoidable precluding ones ability to quantify specific fluorophores based on fluorescence. Similarly, descrimination of in vivo autofluorescence of similar spectral properties to commonly employed NIR dyes, consequent of ingested food comlicates contrast even further. More recently spectral imaging techniques have aided discrimination of fluorophores of similar spectral profiles however, these techniques attenuate much of the light reaching the detector. Time-domain (TD) optical imaging through the use of pulsed laser diodes and time resolved detector system, typically a photo-multiplier tube (PMT), has previously been demonstrated to distinguish between changes in physiological processes such as; tissue pH or calcuim concentration, based on changes in fluorescence lifetime of a fluorescently labelled probe. Here we demonstrate employing a single wave lenght TD optical imaging (eXplore Optix™, ART Inc) the potential to discriminate and quantify combinations of diverse NIR probes of spectrally similar properties but differing fluorescence lifetime on the basis of fluorescence lifetime in appropriate in vitro phantoms. Similarly, we illustrate the ability of this technique to discriminate between endogenous autofluorescence from administered fluorophores in vivo of leukemic cells in liver and spleen, and subsequent distinction of mixtures these fluorophores via their inherent fluorescent lifetimes in vivo.

Nitroreductase, a Near-Infrared Reporter Platform for In Vivo Time-Domain Optical Imaging of Metastatic Cancer

2012 ◽  
Vol 73 (4) ◽  
pp. 1276-1286 ◽  
Author(s):  
Emmet McCormack ◽  
Elisabeth Silden ◽  
Richard M. West ◽  
Tina Pavlin ◽  
David R. Micklem ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Time Domain ◽  
Near Infrared ◽  
Metastatic Cancer

scholarly journals In vivo measure of neonate brain optical properties and hemodynamic parameters by time-domain near-infrared spectroscopy

2017 ◽  
Vol 4 (04) ◽  
pp. 1 ◽  
Author(s):  
Lorenzo Spinelli ◽  
Lucia Zucchelli ◽  
Davide Contini ◽  
Matteo Caffini ◽  
Jacques Mehler ◽  
...  
Keyword(s):  
Optical Properties ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Time Domain ◽  
Near Infrared ◽  
Hemodynamic Parameters

scholarly journals Monitoring Proteins Using In Vivo Near-Infrared Time-Domain Optical Imaging after 2-O-Hexyldiglycerol-Mediated Transfer to the Brain

2010 ◽  
Vol 13 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Petra Hülper ◽  
Christian Dullin ◽  
Wilfried Kugler ◽  
Max Lakomek ◽  
Bernhard Erdlenbruch
Keyword(s):  
Optical Imaging ◽  
Time Domain ◽  
Near Infrared ◽  
The Brain

scholarly journals A Compact Multi-Distance DCS and Time Domain NIRS Hybrid System for Hemodynamic and Metabolic Measurements

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 870
Author(s):  
Caterina Amendola ◽  
Michele Lacerenza ◽  
Mauro Buttafava ◽  
Alberto Tosi ◽  
Lorenzo Spinelli ◽  
...  
Keyword(s):  
Blood Flow ◽  
Hybrid System ◽  
Time Domain ◽  
Near Infrared ◽  
Correlation Spectroscopy ◽  
Optical Signals ◽  
In Vivo Experiments ◽  
Tissue Optical Properties ◽  
Depth Sensitivity

In this work, we present a new multi-distance diffuse correlation spectroscopy (DCS) device integrated with a compact state-of-the-art time domain near infrared spectroscopy (TD-NIRS) device. The hybrid DCS and TD-NIRS system allows to retrieve information on blood flow, tissue oxygenation, and oxygen metabolic rate. The DCS device performances were estimated in terms of stability, repeatability, ability in retrieving variations of diffusion coefficient, influence of the tissue optical properties, effect of varying count rates and depth sensitivity. Crosstalk between DCS and TD-NIRS optical signals was also evaluated. Finally, in vivo experiments (venous and arterial cuff occlusions on the arm) were conducted to test the ability of the hybrid system in measuring blood flow variations.

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