scholarly journals Prior information in fluorescence molecular tomography based on multispectral fluorescence emission

2007 ◽  
Author(s):  
J. Axelsson ◽  
J. Svensson ◽  
S. Andersson-Engels
Keyword(s):  
Prior Information ◽  
Fluorescence Emission ◽  
Fluorescence Molecular Tomography

Subsurface fluorescence molecular tomography with prior information

2014 ◽  
Vol 53 (3) ◽  
pp. 402 ◽  
Author(s):  
Wei He ◽  
Huangsheng Pu ◽  
Guanglei Zhang ◽  
Xu Cao ◽  
Bin Zhang ◽  
...  
Keyword(s):  
Prior Information ◽  
Fluorescence Molecular Tomography

An Image Reconstruction System Applied to High Voltage Microscopy

1975 ◽  
Vol 33 ◽  
pp. 292-293
Author(s):  
D. E. Johnson
Keyword(s):  
High Voltage ◽  
Prior Information ◽  
Block Diagram ◽  
Three Dimensional ◽  
Real Space ◽  
Two Dimensional ◽  
Efficient Manner ◽  
Fourier Methods ◽  
Tilt Series ◽  
Methods Of Reconstruction

Increased specimen penetration; the principle advantage of high voltage microscopy, is accompanied by an increased need to utilize information on three dimensional specimen structure available in the form of two dimensional projections (i.e. micrographs). We are engaged in a program to develop methods which allow the maximum use of information contained in a through tilt series of micrographs to determine three dimensional speciman structure.In general, we are dealing with structures lacking in symmetry and with projections available from only a limited span of angles (±60°). For these reasons, we must make maximum use of any prior information available about the specimen. To do this in the most efficient manner, we have concentrated on iterative, real space methods rather than Fourier methods of reconstruction. The particular iterative algorithm we have developed is given in detail in ref. 3. A block diagram of the complete reconstruction system is shown in fig. 1.

Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy

2017 ◽  
Author(s):  
Pedro M. R. Paulo ◽  
David Botequim ◽  
Agnieszka Jóskowiak ◽  
Sofia Martins ◽  
Duarte M. F. Prazeres ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Emission ◽  
Directed Assembly ◽  
Plasmon Mode ◽  
Correlation Spectroscopy ◽  
Enhanced Fluorescence ◽  
Detection Volume ◽  
Relaxation Component ◽  
Good Agreement ◽  
Confocal Detection

<div> <div> <div> <p>We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon- enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from non-enhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. </p> </div> </div> </div>

Batch Reconstruction from UAV Images with Prior Information

2014 ◽  
Vol 39 (6) ◽  
pp. 834-845 ◽  
Author(s):  
Fu-Sheng GUO ◽  
Wei GAO
Keyword(s):  
Prior Information ◽  
Uav Images
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