Evaluation of aptamers as molecular recognition elements for pathogens using capillary electrophoretic analysis

2006 ◽  
Author(s):  
Sun McMasters ◽  
Dimitra N. Stratis-Cullum
Keyword(s):  
Molecular Recognition ◽  
Electrophoretic Analysis ◽  
Recognition Elements ◽  
Capillary Electrophoretic

scholarly journals CAPILLARY ELECTROPHORETIC ANALYSIS OF LOW-MOLECULAR-MASS OF CA SPECIES IN PHLOEM SAP OF Ricinus communis L.

2010 ◽  
Vol 6 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Noor Fitri ◽  
Björn Thiele ◽  
Klaus Günther ◽  
Buchari Buchari
Keyword(s):  
Molecular Mass ◽  
Ricinus Communis ◽  
Ultra Violet ◽  
Electrophoretic Analysis ◽  
Active Species ◽  
Dihydrogen Phosphate ◽  
Hydrogen Phosphate ◽  
Phloem Sap ◽  
Ricinus Communis L ◽  
Capillary Electrophoretic

A capillary electrophoretic (CE) analysis with ultra-violet (UV) detection was performed for further separation of low-molecular-mass (LMM) calcium species in phloem sap of Ricinus communis L. Two different background electrolytes (BGE) were used for the separation; these are (1) hydrogen phosphate/dihydrogen phosphate buffer containing cetyltrimethylammonium bromide (CTAB) as an electro-osmotic flow (EOF) modifier, and (2) boric acid buffer containing CTAB. Various parameters affecting the analysis, including the composition and pH of the BGE were systematically studied. The sensitivity, resolution, baseline noise, migration time of the species peaks, and reproducibility of the method were evaluated under optimised condition. At least 13 UV-active species were optimally separated within about ten minutes. The optimised measurement condition was also achieved using 10 mM hydrogen phosphate/10 mM dihydrogen phosphate containing 0.5 mM CTAB at pH 8.0 as BGE, and by applying voltage of ‑20 kV and temperature of 14°C. Evidently, the analytical method was successfully used for the separation of LMM calcium species in phloem sap of R. communis L.   Keywords: capillary electrophoresis, calcium species, phloem sap, Ricinus communis

Capillary electrophoretic analysis of ginseng polypeptide

1998 ◽  
Vol 19 (8-9) ◽  
pp. 1270-1274 ◽  
Author(s):  
Hideyuki Kajiwara ◽  
Andrew M. Hemmings
Keyword(s):  
Electrophoretic Analysis ◽  
Capillary Electrophoretic

Application of aptamers as molecular recognition elements in lateral flow assays

2020 ◽  
Vol 593 ◽  
pp. 113574 ◽  
Author(s):  
Ruth Reid ◽  
Bandhan Chatterjee ◽  
Soon Jyoti Das ◽  
Sourav Ghosh ◽  
Tarun Kumar Sharma
Keyword(s):  
Molecular Recognition ◽  
Lateral Flow ◽  
Lateral Flow Assays ◽  
Recognition Elements
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