Manipulation of yeast cells in a microfluidic channel using the GPC-based optical trapping system

2006 ◽  
Author(s):  
Ivan R. Perch-Nielsen ◽  
Peter John Rodrigo ◽  
Jesper Glückstad
Keyword(s):  
Optical Trapping ◽  
Microfluidic Channel ◽  
Yeast Cells

THEORETICAL ANALYSIS AND EXPERIMENT OF A NOVEL DEP CHIP WITH 3-D SILICON ELECTRODES

2005 ◽  
Vol 15 (02) ◽  
pp. 231-236 ◽  
Author(s):  
LIMING YU ◽  
FRANCIS E. H. TAY ◽  
GUOLIN XU ◽  
CIPRIAN ILIESCU ◽  
MARIOARA AVRAM
Keyword(s):  
Theoretical Analysis ◽  
Applied Voltage ◽  
Microfluidic Channel ◽  
The Other ◽  
Yeast Cells ◽  
Applied Potential ◽  
Dielectrophoretic Force ◽  
Joule Effect ◽  
Doped Silicon ◽  
Planar Electrodes

This paper presents a novel dielectrophoresis (DEP) device where the DEP electrodes define the channel walls. This is achieved by fabricating microfluidic channel walls from highly doped silicon so that they can also function as DEP electrodes. Compared with planar electrodes, this device increases the exhibited dielectrophoretic force on the particle, therefore decreases the applied potential and reduces the heating of the solution. A DEP device with triangle electrodes has been designed and fabricated. Compared with the other two configurations, semi-circular and square, triangle electrode presents an increased force, which can decrease the applied voltage and reduce the Joule effect. Yeast cells have been used to for testing the performance of the device.

scholarly journals Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

Lab on a Chip ◽  
2006 ◽  
Vol 6 (8) ◽  
pp. 988 ◽  
Author(s):  
Min Cheol Park ◽  
Jae Young Hur ◽  
Keon Woo Kwon ◽  
Sang-Hyun Park ◽  
Kahp Y. Suh
Keyword(s):  
Microfluidic Channel ◽  
Yeast Cells

Magnetic and Electric Manipulation of a Single Cell in Fluid

2004 ◽  
Vol 820 ◽  
Author(s):  
Hakho Lee ◽  
Tom P. Hunt ◽  
Robert M. Westervelt
Keyword(s):  
Yeast Cell ◽  
Single Cell ◽  
Electric Fields ◽  
Magnetic Beads ◽  
Microfluidic Channel ◽  
Yeast Cells ◽  
Length Scales

AbstractMagnetic and electric manipulation of a single cell in a microfluidic channel was demonstrated using a microelectromagnet matrix and a micropost matrix. The microelectromagnet matrix is two perpendicular arrays of straight wires that are separated and topped by insulating layers. The micropost matrix is an array of post-shaped electrodes embedded in an insulting layer. By controlling the current in each wire of the microelectromagnet matrix or the voltage on each electrode of the micropost matrix, versatile magnetic or electric fields were created on micrometer length scales, controlling the motion of individual cells in fluid. Single or multiple yeast cells attached to magnetic beads were trapped and moved by the microelectromagnet matrix; a single yeast cell was directly trapped and moved by the micropost matrix.

Isolation of single yeast cells by optical trapping

Yeast ◽  
1993 ◽  
Vol 9 (7) ◽  
pp. 723-732 ◽  
Author(s):  
Jos A. Grimbergen ◽  
Koen Visscher ◽  
Daniel S. Gomes De Mesquita ◽  
G. J. Brakenhoff
Keyword(s):  
Optical Trapping ◽  
Yeast Cells

An Integrated 2-D Active Optical Fiber Manipulator With Microfluidic Channel for Optical Trapping and Manipulation

2008 ◽  
Vol 17 (3) ◽  
pp. 548-557 ◽  
Author(s):  
Chia-Wei Lai ◽  
Suz-Kai Hsiung ◽  
Yin-Quan Chen ◽  
Arthur Chiou ◽  
Gwo-Bin Lee
Keyword(s):  
Optical Fiber ◽  
Optical Trapping ◽  
Microfluidic Channel

scholarly journals Numerical and Experimental Investigation on the Optical Manipulation from an Axicon Lensed Fiber

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 187
Author(s):  
Wu Zhang ◽  
Yanxiao Lin ◽  
Yusong Gao ◽  
Zekai Guo ◽  
Xiangling Li ◽  
...  
Keyword(s):  
Experimental Investigation ◽  
Fluid Flow ◽  
Optical Trapping ◽  
Incident Light ◽  
Microfluidic Channel ◽  
Flow Speed ◽  
Optical Force ◽  
Optical Manipulation ◽  
Incident Wavelength ◽  
Polystyrene Microsphere

Here we numerically and experimentally studied the optical trapping on a microsphere from an axicon lensed fiber (ALF). The optical force from the fiber with different tapered lengths and by incident light at different wavelengths is calculated. Numerically, the microsphere can be trapped by the fiber with tapered outline y=±x/0.5 and y=±x at a short incident wavelength of 900 nm. While for the fiber with tapered outline y=±x/2, the microsphere can be trapped by the light with longer wavelength of 1100 nm, 1300 nm, or 1500 nm. The optical trapping to a polystyrene microsphere is experimentally demonstrated in a microfluidic channel and the corresponding optical force is derived according to the fluid flow speed. This study can provide a guidance for future tapered fibre design for optical trapping to microspheres.

scholarly journals Optical trapping of microparticles and yeast cells at ultra-low intensity by intracavity nonlinear feedback forces

2020 ◽  
Author(s):  
Fatemeh Kalantarifard ◽  
Parviz Elahi ◽  
Ghaith Makey ◽  
Bürçin Ünlü ◽  
Onofrio Maragò ◽  
...  
Keyword(s):  
Optical Trapping ◽  
Yeast Cells ◽  
Nonlinear Feedback ◽  
Low Intensity

Measurement of pH-dependent surface-enhanced hyper-Raman scattering at desired positions on yeast cells via optical trapping

The Analyst ◽  
2017 ◽  
Vol 142 (20) ◽  
pp. 3967-3974 ◽  
Author(s):  
Yasutaka Kitahama ◽  
Hiroaki Hayashi ◽  
Tamitake Itoh ◽  
Yukihiro Ozaki
Keyword(s):  
Silver Nanoparticles ◽  
Raman Scattering ◽  
Laser Beam ◽  
Optical Trapping ◽  
Near Infrared ◽  
Yeast Cells ◽  
Infrared Laser Beam ◽  
Surface Enhanced ◽  
Ph Dependent ◽  
Optically Trapped

At desired positions on yeast, pH-dependent surface-enhanced hyper-Raman scattering (SEHRS) spectra were recorded by focusing a near-infrared laser beam while silver nanoparticles (AgNPs) with 4-mercaptobenzoic acid (p-MBA) were simultaneously optically trapped.

scholarly journals Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 270
Author(s):  
Honeyeh Matbaechi Ettehad ◽  
Christian Wenger
Keyword(s):  
Cell Separation ◽  
Microfluidic Channel ◽  
Microfluidic System ◽  
Yeast Cells ◽  
Cell Manipulation ◽  
Cmos Process ◽  
Label Free ◽  
Electrode Arrays ◽  
A Cell ◽  
Silicon Based

This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers excellent benefits in terms of cost, operational power, and especially easy electrode integration with the CMOS architecture, and requiring label-free sample preparation. This can increase the likeliness of using DEP in practical settings. In this work the DEP force was generated using an interdigitated electrode arrays (IDEs) placed on the bottom of a CMOS-based silicon microfluidic channel. This system was primarily used for the immobilization of yeast cells using DEP. This study validated the system for cell separation applications based on the distinct responses of live and dead cells and their surrounding media. The findings confirmed the device’s capability for efficient, rapid and selective cell separation. The viability of this CMOS embedded microfluidic for dielectrophoretic cell manipulation applications and compatibility of the dielectrophoretic structure with CMOS production line and electronics, enabling its future commercially mass production.

scholarly journals Optical trapping and surgery of living yeast cells using a single laser

2008 ◽  
Vol 79 (10) ◽  
pp. 103705 ◽  
Author(s):  
Jun Ando ◽  
Godofredo Bautista ◽  
Nicholas Smith ◽  
Katsumasa Fujita ◽  
Vincent Ricardo Daria
Keyword(s):  
Optical Trapping ◽  
Yeast Cells ◽  
Single Laser
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