3D analysis of bone formation around titanium implants using micro computed tomography (μCT)

2006 ◽  
Author(s):  
Ricardo Bernhardt ◽  
Dieter Scharnweber ◽  
Bert Müller ◽  
Felix Beckmann ◽  
Jürgen Goebbels ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Formation ◽  
Titanium Implants ◽  
3D Analysis ◽  
Micro Computed Tomography

scholarly journals Parathyroid hormone enhances gap healing and osseointegration in orthopedic porous coated titanium implants: a correlative micro-computed tomographic, histomorphometric and biomechanical analysis

2022 ◽  
Vol 23 (1) ◽  
Author(s):  
Xinlin Gao ◽  
Yang Meng ◽  
Dingjun Hao ◽  
Hao Liu
Keyword(s):  
Computed Tomography ◽  
Parathyroid Hormone ◽  
Bone Formation ◽  
Low Dose ◽  
Titanium Implants ◽  
Biomechanical Analysis ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Pull Out ◽  
Custom Made

Abstract Background Parathyroid hormone, with its anabolic effect on bone formation, has shown excellent outcomes of curing postmenopausal osteoporosis as well as enhancing osseointegration around orthopaedic and stomatologic implants.The purpose of the present study is to test if low-dose intermittent PTH (1–34) treatment could achieve a satisfactory osseointegration in 2-mm peri-implant gaps, as to provide a new idea for improving the stability of such prosthesis, which will be of great clinical value. Methods A custom-made titanium implant was implanted on the calvarium of New Zealand White rabbits. 48 male rabbits were randomly divided into control and PTH group. PTH group received subcutaneous injection of PTH (20 μg/day, 5 days/week). Animals were sacrificed at 4 and 8 weeks after surgery. Quantitative micro-computed tomography, histology and biomechanical pull-out testing were performed to evaluate the gap healing at implantation site. Results Analysis of micro-computed tomography demonstrated that PTH group achieved more new bone formation in 2-mm gaps and on bone-implant interface. Quantitatively, significant differences were observed between two groups in regard to BIC and BV/TV at each time-point. Histological staining revealed that PTH group had a superiority in trabecular number, thickness, separation and better osseointegration compared to control group. As for biomechanical pull-out testing, PTH group also showed significant improvement of ultimate force than control group. Conclusions Low-dose intermittent administration of PTH for 4 and 8 weeks enhances early osseointegration and fixation of orthopedic implants surrounded by a 2-mm gap in terms of increased bone regeneration and mechanical stability. These findings suggest PTH a potential for reducing the postoperative complications of implants by improving bone healing at peri-implant gaps.

scholarly journals 3D analysis of chondron morphology of human and rat articular cartilage from micro-computed tomography

2016 ◽  
Vol 24 ◽  
pp. S298 ◽  
Author(s):  
I. Kestilä ◽  
M.A. Finnilä ◽  
S.S. Karhula ◽  
L. Rieppo ◽  
P. Lehenkari ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Articular Cartilage ◽  
3D Analysis ◽  
Micro Computed Tomography

scholarly journals Quantitative 3D analysis of the canal network in cortical bone by micro-computed tomography

2003 ◽  
Vol 274B (1) ◽  
pp. 169-179 ◽  
Author(s):  
D.M.L. Cooper ◽  
A.L. Turinsky ◽  
C.W. Sensen ◽  
B. Hallgrímsson
Keyword(s):  
Computed Tomography ◽  
Cortical Bone ◽  
3D Analysis ◽  
Micro Computed Tomography ◽  
Canal Network

Quantification of bone formation and resorption rates by in vivo micro-computed tomography

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S245 ◽  
Author(s):  
F.A. Gerhard⁎ ◽  
F.M. Lambers ◽  
G. Kuhn ◽  
R. Muller
Keyword(s):  
Computed Tomography ◽  
Bone Formation ◽  
Micro Computed Tomography

scholarly journals Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation

2019 ◽  
Vol 20 (23) ◽  
pp. 6042 ◽  
Author(s):  
Anqi Huang ◽  
Yoshitomo Honda ◽  
Peiqi Li ◽  
Tomonari Tanaka ◽  
Shunsuke Baba
Keyword(s):  
Computed Tomography ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Bone Formation ◽  
Matrix Metalloproteinase ◽  
Epigallocatechin Gallate ◽  
Bone Defects ◽  
Micro Computed Tomography ◽  
Immunohistological Staining

Matrix metalloproteinase (MMP)-2 and MMP-9 are well-known gelatinases that disrupt the extracellular matrix, including gelatin. However, the advantages of modulating MMP expression in gelatin-based materials for applications in bone regenerative medicine have not been fully clarified. In this study, we examined the effects of epigallocatechin gallate (EGCG), a major polyphenol catechin isolated from green tea, on MMP expression in gelatin sponges and its association with bone formation. Four gelatin sponges with or without EGCG were prepared and implanted into bone defects for up to 4 weeks. Histological and immunohistological staining were performed. Micro-computed tomography was used to estimate the bone-forming capacity of each sponge. Our results showed that EGCG integration attenuated MMP-2 (70.6%) and -9 expression (69.1%) in the 1 week group, increased residual gelatin (118.7%), and augmented bone formation (101.8%) in the 4 weeks group in critical-sized bone defects of rat calvaria compared with vacuum-heated gelatin sponges without EGCG. Moreover, vacuum-heated gelatin sponges with EGCG showed superior bone formation compared with other sponges. The results indicated that integration of EGCG in gelatin-based materials modulated the production and activity of MMP-2 and -9 in vivo, thereby enhancing bone-forming capacity.

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