Characterization of a star/planet simulator for evaluating extrasolar planet detection techniques

2006 ◽  
Author(s):  
Stephen E. Kendrick ◽  
Christopher Robb ◽  
Dennis Ebbets ◽  
Paul Atcheson
Keyword(s):  
Extrasolar Planet ◽  
Planet Detection ◽  
Detection Techniques

scholarly journals Characterization of epididymal and testicular histologic lesions and use of immunohistochemistry and PCR on formalin-fixed tissues to detect Brucella canis in male dogs

2021 ◽  
pp. 104063872098688
Author(s):  
Andrea M. Camargo-Castañeda ◽  
Lauren W. Stranahan ◽  
John F. Edwards ◽  
Daniel G. Garcia-Gonzalez ◽  
Leonardo Roa ◽  
...  
Keyword(s):  
Accurate Diagnosis ◽  
Testicular Atrophy ◽  
Detection Techniques ◽  
Formalin Fixed Paraffin ◽  
Brucella Canis ◽  
Formalin Fixed Paraffin Embedded ◽  
Variable Sensitivity ◽  
Ffpe Tissues ◽  
Formalin Fixed

In male dogs, Brucella canis frequently causes epididymitis, ultimately resulting in testicular atrophy and infertility. Although B. canis predominantly affects the epididymis, the misleading term “orchitis” is still commonly used by clinicians. Of additional concern, diagnosis in dogs remains challenging because of variable sensitivity and specificity of serologic assays and fluctuations in bacteremia levels in infected dogs, reducing the sensitivity of blood culture. We describe here the histologic lesions in the scrotal contents of 8 dogs suspected of being infected with B. canis and clinically diagnosed with orchitis. We explored the possibility of using immunohistochemistry (IHC) and real-time PCR (rtPCR) in formalin-fixed, paraffin-embedded (FFPE) tissues to detect the presence of B. canis. Epididymitis of variable chronicity was identified in all 8 dogs, with only 3 also exhibiting orchitis. Using rtPCR, the presence of B. canis was identified in 4 of 8 dogs, with 3 of these 4 dogs also positive by IHC. These results suggest that rtPCR and IHC are promising techniques that can be used in FFPE tissues to detect B. canis when other detection techniques are unavailable. Additionally, accurate recognition of epididymitis rather than orchitis in suspect cases could aid in accurate diagnosis.

scholarly journals A Novel Triple-Fluorescent HCMV Strain Reveals Gene Expression Dynamics and Anti-Herpesviral Drug Mechanisms

2021 ◽  
Vol 10 ◽  
Author(s):  
Ulfert Rand ◽  
Tobias Kubsch ◽  
Bahram Kasmapour ◽  
Luka Cicin-Sain
Keyword(s):  
Gene Expression ◽  
Hcmv Infection ◽  
Viral Fitness ◽  
High Temporal Resolution ◽  
Detection Techniques ◽  
Drug Candidates ◽  
New Drug ◽  
Gene Expression Dynamics ◽  
Identification And Characterization

Human Cytomegalovirus (HCMV) infection may result in severe outcomes in immunocompromised individuals such as AIDS patients, transplant recipients, and neonates. To date, no vaccines are available and there are only few drugs for anti-HCMV therapy. Adverse effects and the continuous emergence of drug-resistance strains require the identification of new drug candidates in the near future. Identification and characterization of such compounds and biological factors requires sensitive and reliable detection techniques of HCMV infection, gene expression and spread. In this work, we present and validate a novel concept for multi-reporter herpesviruses, identified through iterative testing of minimally invasive mutations. We integrated up to three fluorescence reporter genes into replication-competent HCMV strains, generating reporter HCMVs that allow the visualization of replication cycle stages of HCMV, namely the immediate early (IE), early (E), and late (L) phase. Fluorescent proteins with clearly distinguishable emission spectra were linked by 2A peptides to essential viral genes, allowing bicistronic expression of the viral and the fluorescent protein without major effects on viral fitness. By using this triple color reporter HCMV, we monitored gene expression dynamics of the IE, E, and L genes by measuring the fluorescent signal of the viral gene-associated fluorophores within infected cell populations and at high temporal resolution. We demonstrate distinct inhibitory profiles of foscarnet, fomivirsen, phosphonoacetic acid, ganciclovir, and letermovir reflecting their mode-of-action. In conclusion, our data argues that this experimental approach allows the identification and characterization of new drug candidates in a single step.

scholarly journals Simulation of Extrasolar Planet Detection with Rotationally Shearing Interferometer at 10 µm

Proceedings ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 44
Author(s):  
Strojnik ◽  
Bravo-Medina
Keyword(s):  
Extrasolar Planet ◽  
Planet Detection ◽  
Dove Prism ◽  
Fringe Patterns ◽  
Angle Of Rotation

We simulate the interferometric fringe patterns for the case of an extrasolar planet system with two planets. We show that the incidence distributions become more complicated, yet their interpretation is feasible. The generalized fringe distributions rotate when the Dove prism is rotated. Likewise, the number of fringes changes with the angle of rotation of the Dove prism.

Simulation of Leak Noise Propagation and Detection Using COMSOL Multiphysics

2016 ◽  
Author(s):  
Wadie R. Chalgham ◽  
Abdennour C. Seibi ◽  
Fathi Boukadi
Keyword(s):  
Experimental Data ◽  
Fluid Velocity ◽  
Sound Level ◽  
Velocity Variation ◽  
Comsol Multiphysics ◽  
Widespread Application ◽  
Detection Techniques ◽  
Pipeline Inspection ◽  
Fluid Characteristics

Pipelines leak detection represents an essential aspect in pipeline rehabilitation to avoid any unexpected failure. Several detection techniques have been adopted and received a widespread application in pipeline inspection nowadays but still present a major challenge to field operators. This paper presents an attempt to develop correlations between leaks and their effect on the fluid characteristics inside the pipeline such as fluid velocity, variation of pressure and sound level due to the presence of leaks along the pipeline. Characterization of these parameters and how they propagate with respect to time from the leak source will allow the development of a solution to detect leaks and quantify the amount of fluid being lost. This paper aims at conducting an experimental investigation to determine the sound level for specified leak sizes. The experimental data was used in COMSOL Multiphysics to simulate various fluid flow scenarios inside a 2 in. (5.08 cm.) pipe with different leak sizes.

Compared molecular characterization of hyaluronan using multiple-detection techniques

Polymer ◽  
2011 ◽  
Vol 52 (24) ◽  
pp. 5648-5658 ◽  
Author(s):  
Tu Luan ◽  
Yapeng Fang ◽  
Saphwan Al-Assaf ◽  
Glyn O. Phillips ◽  
Hongbin Zhang
Keyword(s):  
Molecular Characterization ◽  
Detection Techniques ◽  
Multiple Detection

The photometric method of extrasolar planet detection revisited

1994 ◽  
Vol 212 (1-2) ◽  
pp. 335-348 ◽  
Author(s):  
Alan Hale ◽  
Laurance R. Doyle
Keyword(s):  
Extrasolar Planet ◽  
Photometric Method ◽  
Planet Detection

scholarly journals Efficient conjugation of anti-HBsAg antibody to modified core-shell magnetic nanoparticles (Fe3O4@SiO2/NH2)

Bioimpacts ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 237-244
Author(s):  
Shahram Parvaneh ◽  
Fatemeh Khademi ◽  
Gisya Abdi ◽  
Abdolhamid Alizadeh ◽  
Ali Mostafaie
Keyword(s):  
Magnetic Nanoparticles ◽  
Sodium Periodate ◽  
Binding Capacity ◽  
High Capacity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Core Shell ◽  
Detection Techniques ◽  
B Virus ◽  
Further Development

Introduction: Further development of magnetic-based detection techniques could be of significant use in increasing the sensitivity of detection and quantification of hepatitis B virus (HBV) infection. The present work addresses the fabrication and characterization of a new bio-nano composite based on the immobilization of goat anti-HBsAg antibody on modified core-shell magnetic nanoparticles (NPs) by (3-aminopropyl) triethoxysilane (APTES), named Fe3O4@SiO2/NH2, and magnetic NPs modified by chitosan (Fe3O4@CS). Methods: At the first step, Fe3O4 was modified with the silica and APTES (Fe3O4@SiO2/NH2) and chitosan (Fe3O4@CS) separately. The goat anti-HBsAg antibody was activated by two different protocols: Sodium periodate and EDC-NHS. Then the resulted composites were conjugated with activated goat anti-HBsAg IgG. An external magnet collected Bio-super magnetic NPs (BSMNPs) and the remained solution was analyzed by the Bradford method to check the amount of attached antibody to the surface of BSMNPs. Results: The findings indicated that activation of antibodies by sodium periodate method 15-17 µg antibody immobilized on 1 mg of super magnetic nanoparticles (SMNPs). However, in the EDC-NHS method, 8-10 µg of antibody was conjugated with 1 mg of SMNPs. The resulting bio-magnetic NPs were applied for interaction with the HBsAg target using enzyme-linked immunosorbent assay (ELISA). About 1 µg antigen attached to 1 mg SMNPs, which demonstrated that the fabricated materials are applicable in the detection scope of HBsAg. Conclusion: In the present study, we developed new antibody-conjugated magnetic NPs for the detection of HBsAg using an efficient conjugation strategy. The results demonstrated that the binding capacity of Fe3O4@SiO2/NH2 was comparable with commercially available products. Our designed method for conjugating anti-HBsAg antibody to a magnetic nanoparticle opens the way to produce a high capacity of magnetic NPs.

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