scholarly journals Nanosurgery of sub-cellular organelles in living cells using a femtosecond laser oscillator

2006 ◽  
Author(s):  
Wataru Watanabe ◽  
Tomoko Shimada ◽  
Sachihiro Matsunaga ◽  
Hiroshi Ishii ◽  
Tsunehito Higashi ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Living Cells ◽  
Laser Oscillator ◽  
Cellular Organelles

scholarly journals Femtosecond Laser Manipulation of Subcellular Organelles in Living Cells

2005 ◽  
Author(s):  
Wataru Watanabe ◽  
Timoko Shimada ◽  
Kazuyoshi Itoh ◽  
Sachihiro Matsunaga ◽  
Kiichi Fukui
Keyword(s):  
Femtosecond Laser ◽  
Living Cells ◽  
Subcellular Organelles ◽  
Laser Manipulation

Coherent Raman Spectroscopy with a Fiber-Format Femtosecond Laser Oscillator

2010 ◽  
Author(s):  
G. Cerullo ◽  
M. Marangoni ◽  
P. M. Champion ◽  
L. D. Ziegler
Keyword(s):  
Raman Spectroscopy ◽  
Femtosecond Laser ◽  
Laser Oscillator ◽  
Coherent Raman

scholarly journals Characterization of symmetric [3 x 3] directional couplers fabricated by direct writing with a femtosecond laser oscillator

2006 ◽  
Vol 14 (6) ◽  
pp. 2335 ◽  
Author(s):  
Kenya Suzuki ◽  
Vikas Sharma ◽  
James G. Fujimoto ◽  
Erich P. Ippen ◽  
Yusuke Nasu
Keyword(s):  
Femtosecond Laser ◽  
Direct Writing ◽  
Laser Oscillator ◽  
Directional Couplers

Visualizing formation and dynamics of vacuoles in living cells using contrasting dextran-bound indicator: endocytic and nonendocytic vacuoles

2007 ◽  
Vol 293 (6) ◽  
pp. G1333-G1338 ◽  
Author(s):  
Svetlana G. Voronina ◽  
Mark W. Sherwood ◽  
Oleg V. Gerasimenko ◽  
Ole H. Petersen ◽  
Alexei V. Tepikin
Keyword(s):  
Endoplasmic Reticulum ◽  
Confocal Microscopy ◽  
Acinar Cells ◽  
Cell Types ◽  
Living Cells ◽  
Pancreatic Acinar Cells ◽  
Extracellular Solution ◽  
Whole Cell Patch Clamp ◽  
Patch Pipette ◽  
Cellular Organelles

Here we describe a technique that allows us to visualize in real time the formation and dynamics (fusion, changes of shape, and translocation) of vacuoles in living cells. The technique involves infusion of a dextran-bound fluorescent probe into the cytosol of the cell via a patch pipette, using the whole-cell patch-clamp configuration. Experiments were conducted on pancreatic acinar cells stimulated with supramaximal concentrations of cholecystokinin (CCK). The vacuoles, forming in the cytoplasm of the cell, were revealed as dark imprints on a bright fluorescence background, produced by the probe and visualized by confocal microscopy. A combination of two dextran-bound probes, one infused into the cytosol and the second added to the extracellular solution, was used to identify endocytic and nonendocytic vacuoles. The cytosolic dextran-bound probe was also used together with a Golgi indicator to illustrate the possibility of combining the probes and identifying the localization of vacuoles with respect to other cellular organelles in pancreatic acinar cells. Combinations of cytosolic dextran-bound probes with endoplasmic reticulum (ER) or mitochondrial probes were also used to simultaneously visualize vacuoles and corresponding organelles. We expect that the new technique will also be applicable and useful for studies of vacuole dynamics in other cell types.

Coherent Raman Microscopy with a Fiber-Format Femtosecond Laser Oscillator

2010 ◽  
Author(s):  
M. Marangoni ◽  
A. Gambetta ◽  
V. Kumar ◽  
G. Grancini ◽  
D. Polli ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Raman Microscopy ◽  
Laser Oscillator ◽  
Coherent Raman

Coherent Raman Microscopy with a Fiber-Format Femtosecond Laser Oscillator

2010 ◽  
Author(s):  
A. Gambetta ◽  
V. Kumar ◽  
G. Grancini ◽  
D. Polli ◽  
C. Manzoni ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Raman Microscopy ◽  
Laser Oscillator ◽  
Coherent Raman

scholarly journals Femtosecond laser manipulation of subcellular organelles in living cells

2005 ◽  
Author(s):  
Wataru Watanabe ◽  
Tomoko Shimada ◽  
Kazuyoshi Itoh ◽  
Sachihiro Matsunaga ◽  
Kiichi Fukui
Keyword(s):  
Femtosecond Laser ◽  
Living Cells ◽  
Subcellular Organelles ◽  
Laser Manipulation

scholarly journals Antenna-coupled spintronic terahertz emitters driven by a 1550 nm femtosecond laser oscillator

2019 ◽  
Vol 115 (2) ◽  
pp. 022405 ◽  
Author(s):  
U. Nandi ◽  
M. S. Abdelaziz ◽  
S. Jaiswal ◽  
G. Jakob ◽  
O. Gueckstock ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Oscillator ◽  
Terahertz Emitters
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