Luminescent probe in the study of surfactant-induced structural changes in serum albumin in human blood plasma

2005 ◽  
Author(s):  
A. G. Melnikov ◽  
A. B. Pravdin ◽  
V. I. Kochubey ◽  
G. V. Melnikov
Keyword(s):  
Serum Albumin ◽  
Blood Plasma ◽  
Human Blood ◽  
Structural Changes ◽  
Human Blood Plasma ◽  
Luminescent Probe

scholarly journals Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4388
Author(s):  
Tomáš Suchý ◽  
Martin Bartoš ◽  
Radek Sedláček ◽  
Monika Šupová ◽  
Margit Žaloudková ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Mass Loss ◽  
Human Blood ◽  
Structural Changes ◽  
Mechanical Tests ◽  
Alternative Methods ◽  
Human Blood Plasma ◽  
Tissue Engineering Scaffolds ◽  
Before And After ◽  
The Media

This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, human blood plasma, and phosphate buffer saline. A comparison was performed of the effects of the media on collagen scaffolds. The mechanical and structural effects of the media were determined via the application of compression mechanical tests, the determination of mass loss, and image and micro-CT analyses. The adsorption of various components from the media was characterized employing energy-dispersive spectrometry. The phase composition of the materials before and after exposure was determined using X-ray diffraction. Infrared spectroscopy was employed for the interpretation of changes in the collagen secondary structure. Major differences in terms of the mechanical properties and mass loss were observed between the three media. Conversely, only minor structural changes were detected. Since no general recommendation exists for selecting the simulated body environment, it is necessary to avoid the simplification of the results and, ideally, to utilize alternative methods to describe the various aspects of degradation processes that occur in the media.

scholarly journals Human blood plasma factors affect the adhesion kinetics of Staphylococcus aureus to central venous catheters

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Gubesh Gunaratnam ◽  
Christian Spengler ◽  
Simone Trautmann ◽  
Philipp Jung ◽  
Johannes Mischo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Serum Albumin ◽  
Blood Plasma ◽  
Human Blood ◽  
Central Venous Catheters ◽  
Human Blood Plasma ◽  
Adhesion Forces ◽  
Central Venous ◽  
Major Step ◽  
Initial Adhesion

AbstractStaphylococcus aureus is a common cause of catheter-related blood stream infections (CRBSI). The bacterium has the ability to form multilayered biofilms on implanted material, which usually requires the removal of the implanted medical device. A first major step of this biofilm formation is the initial adhesion of the bacterium to the artificial surface. Here, we used single-cell force spectroscopy (SCFS) to study the initial adhesion of S. aureus to central venous catheters (CVCs). SCFS performed with S. aureus on the surfaces of naïve CVCs produced comparable maximum adhesion forces on three types of CVCs in the low nN range (~ 2–7 nN). These values were drastically reduced, when CVC surfaces were preincubated with human blood plasma or human serum albumin, and similar reductions were observed when S. aureus cells were probed with freshly explanted CVCs withdrawn from patients without CRBSI. These findings indicate that the initial adhesion capacity of S. aureus to CVC tubing is markedly reduced, once the CVC is inserted into the vein, and that the risk of contamination of the CVC tubing by S. aureus during the insertion process might be reduced by a preconditioning of the CVC surface with blood plasma or serum albumin.

Dual Layer Mixed Matrix Hollow Fiber Membranes for Outside-In Filtration of Human Blood Plasma

2020 ◽  
Author(s):  
O.E.M. ter Beek ◽  
M.K. van Gelder ◽  
C. Lokhorst ◽  
D.H.M. Hazenbrink ◽  
B.H. Lentferink ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Hollow Fiber ◽  
Human Blood Plasma ◽  
Hollow Fiber Membranes ◽  
Mixed Matrix ◽  
Fiber Membranes

scholarly journals Properties of triglyceride-rich and cholesterol-rich lipoproteins in the remnant-like particle fraction of human blood plasma

2002 ◽  
Vol 43 (3) ◽  
pp. 365-374
Author(s):  
Elisa Campos ◽  
Leila Kotite ◽  
Patricia Blanche ◽  
Yasushi Mitsugi ◽  
Philip H. Frost ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Human Blood Plasma ◽  
Particle Fraction
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